Báo cáo khoa học: New roles of ﬂavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin pot

... MINIREVIEW New roles of ﬂavoproteins in molecular cell biology: Histone demethylase LSD1 and chromatin Federico Forneris1, Elena Battaglioli2, Andrea Mattevi1 and Claudia Binda11 ... branches off in MAO A and B (Fig. 3A). The SWIRM domain is a motif com-monly found in chromatin enzymes and is responsiblefor protein–protein and DNA–protein interactions[36]. The name of this ... methylated Lys4 aminogroup.Multidomain organization of LSD1 and its enzymatic activity in the chromatin contextGenes coding for LSD1 ⁄ K -demethylase 1 proteinsexist only in eukaryotes and have undergone...

Báo cáo khoa học: New roles of ﬂavoproteins in molecular cell biology: Blue-light active ﬂavoproteins studied by electron paramagnetic resonance pptx

... FEBS 4303MINIREVIEW New roles of flavoproteins in molecular cell biology: Blue-light active flavoproteins studied by electronparamagnetic resonanceErik Schleicher1, Robert Bittl2 and Stefan ... otherproteins involved in intracellular signaling or localiza-tion [24,25]. In photolyases and cryptochromes, radicals and radical pairs play a prominent role in their function. In nearly all ... modification of the cofactor-binding site at the 8a-methyl group and at the linkage of the ribityl side chain. Hence, as a first result, struc-tural information regarding the distance and locationof...

Báo cáo khoa học: New roles of flavoproteins in molecular cell biology: An unexpected role for quinone reductases as regulators of proteasomal degradation pptx

... are involved in recognition of the polyubiquitinated protein substrates, unfolding of the condemned protein [24], removing ubiquitin chainsfor recycling [25,26] and opening an axial gate into ... compilation ª 2009 FEBSMINIREVIEW New roles of ﬂavoproteins in molecular cell biology: Anunexpected role for quinone reductases as regulators of proteasomal degradationSonja Sollner and Peter MacherouxTechnische ... be downregulated in several carcinomas.The protein was shown to be a major player in cellularstress responses, including cell- cycle arrest, apoptosis, chromatin remodelling and DNA repair [53]....

Báo cáo khoa học: Regulatory roles of hyaluronan in health and disease ppt

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Tài liệu Báo cáo khóa học: New activities of a catalytic antibody with a peroxidase activity ppt

... of the Fe(II) spin state and noreplacement of any of the two axial ligands of the iron,His18 or RNO, by an amino acid side-chain of theantibody protein.Binding site topology of antibody 3A3Figure ... the binding of MP8 occurred through interactions of its carboxylate substituents with amino acids of theantibody, and that the protein provided a partial sterichindrance of the distal face of ... formed not only in vitro, but also in vivo, during the oxidative metabolism of drugs containing an amine function, such as amphetamineor macrolids [20], and which lead to an inhibition of thecatalytic...

Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc

... he redox potential of the haem, rate constants o f CO, O2 and CN b inding tohaem, and CD spectra in the Soret region [15–18],suggesting that this residue is critical for maintaining theelectronic ... side and/ or structure in t erms of cyanide binding in the Fe(III) complex.Redox potentialsThe redox state of haem is related to the PDE activity of Ec DOS, since it is a haem redox-sensing enzyme ... protein.Additionally, spin states of the Fe(III), Fe(II) and Fe(II)-CO complexes of Asp40 mutants were similar tothose of the wild-type enzyme in that all mutant proteinswere in the six-coordinated...

Báo cáo khoa học: Respective roles of the catalytic domains and C-terminal tail peptides in the oligomerization and secretory trafﬁcking of human acetylcholinesterase and butyrylcholinesterase potx

... However, in the case of Bb, the cellular activity was decreased and secretionwas increased, suggesting that the presence of this cys-teine retains the enzyme intracellularly. In case of Aa,the cellular ... acetylthiocholine. The rates of hydrolysis of acetylthiocholine and butyrylthiocholine(at 6 mm) by BChE were approximately 14% and 24% of the rate of hydrolysis of acetylthiocholine(at 2 mm) by AChE. In uence ... BChE contains a proline-rich peptide fromlamellipodin [14]. In conclusion, the catalytic domains of cholinesterases and their C-terminal t peptides constitute modules of quaternary interactions...

Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc

... also beinvolved. In multicellular organisms, GATA-bindingfactors play critical roles in development, including cell- fate speciﬁcation, regulation of differentiation, and control of cell proliferation ... second intronincluded nucleotides 210–253 in each gene. Excludingintrons and stop codons, the c-T1 and c-T2 codingregions were each 1383 bp in length and predictedproteins of 461 amino acids. ... for each cell ⁄ antigen combination. (A–D) Ventral view of cell 1 in late vegetative stage; (E–H) dorsal view of cell 1; and (I–L) ventral view of cell 2 in mitosis. The arrowhead in (J) indicates...

Báo cáo khoa học: New application of ﬁreﬂy luciferase ) it can catalyze the enantioselective thioester formation of 2-arylpropanoic acid docx

... theremaining acid and flurbiprofen as an internal stand-ard using reversed phase HPLC analysis. According tothe Lineweaver–Burk plot, the Km and Vmaxvalues forracemic ketoprofen were 0.22 mm and ... not investigated in detail and only threekinds of substrate, ibuprofen, fenoprofen, and flurbi-profen, were examined [16]. With these substrates,flurbiprofen could not be used by LACS1. In the ... enzyme that concerns in the bioluminescence reaction. It catalyzes the oxida-tion of firefly luciferin with molecular oxygen in thepresence of ATP and Mg2+, resulting in luminescence[1–3]. The...

Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf

... to30 kDa in size. Subunits of all known GST structuresexhibit a two-domain fold, the N-terminal domain and C-terminal domain, including the highly conservedGSH-binding site (G-site) and the ... hypothesis.Experimental procedures Molecular cloning of sweet orange GSTU1 and GSTU2Cloning of sweet orange GSTU1 and GSTU2 genes and their transfer into the expression vector pEXP1–DEST(Invitrogen, ... demonstrate the crucial role of both Ser67 and Glu66 in GSH binding, corroboratethese ﬁndings [28]. In contrast, the H-sites of GSTsexhibit a low degree of sequence identity, and henceunique structures...

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