Báo cáo khoa học: Lysosomal localization of GLUT8 in the testis – the EXXXLL motif of GLUT8 is sufficient for its intracellular sorting via AP1- and AP2-mediated interaction docx
Tài liệu Báo cáo khoa học: Restricted localization of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase at the neuromuscular junctions – contribution and expression from motor neurons doc
... reduc-tion of G1 and G4forms in the spinal cord and of G1,G4 and A12forms in the tibialis (Fig. 8C). Thus,denervation induced a decrease in PRiMA and G4AChE in the spinal cord and muscle.To ... determine the local-ization of PRiMA-linked G4AChE, sections of tibialismuscle were triple stained for PRiMA, SV48 and AChR. The staining of PRiMA was coincident withthat of SV48, rather ... spinal cord about10 days after birth (Fig. 5E).To determine the origin of AChE in the spinalcord, the lumbar region of the spinal cord wassectioned and stained with the PRiMA antibody. The label...
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Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
... both the internal region of exon V that is absent from the inactive PAOh4 isoform, as well as exon VIa, which is absent from the active isoform, SMO ⁄ PAOh1. SMO5 and the active, mouse isoform, ... components of the FAD-bindingdomain, and are therefore essential for catalysis[28,3 3–3 5].Kinetic properties of SMO5Polyamine oxidase activity of SMO5 and SMO ⁄PAOh1 was measured using increasing ... proteins, no homologue of the mousemSMOl, which is essentially a combination of the human isoforms 1 and 4, had been identified. The dis-covery of its existence in the mouse suggested the like-lihood...
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Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx
... attack of the L-hOrn3a-amino group onto l-haOrn4. Takinginto account that the synthetases involved in the bio-synthesis of the DKP-inheriting toxins thaxtomin and fumitremorgin also contain ... characterization of these compounds is growing and may result in the synthesis of specificinhibitors based on the structure of the pathogensiderophore [16].A promising approach for the isolation of secondarymetabolites, ... beenapplied in the discovery of orfamide A [17]. The accu-rate prediction of adenylation domain specificity wasfound to be crucial for successful mining and structuralprediction and is the basis of the...
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Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf
... Bottom-up proteolysis destroys the information on the size of the protein and the connectivities of the pep-tide fragments, but it has no size limit for protein digestion. In contrast, the top-down ... iso-topically labeling the proteins from one and compar-ing in their mixture the corresponding peaks of theirrespective peptides, usually differing by three ormore mass units [ 9–1 2]. The kinetic deamidationstudy ... in the center of the protein to formadditional loose ends to be denatured out of the ball of spaghetti. The ESI spectrum of the 200 kDa human comple-ment C4 glycoprotein (of 1714 residues in...
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Tài liệu Báo cáo khoa học: Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) doc
... outline of the localization and transport of silicatein in the extraspi-cular space is given in Fig. 6. The data summarized here provide, for the firsttime, enzyme kinetic data for silicatein, ... bonds, the concentration of the released product p-aminophenol was determinedat a wavelength of 300 nm, which is in the trailingedge of the main absorption bands under the condi-tions used. Another ... formation of the spicules proceeds. In the spicule-forming cells, the sclerocytes, the first layers of the silica shell of the spicules are formed around the silicatein-based axial filament in specific...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... P2Y12-dependent part of the Ca2+response, indicating thatPI3-Kß is the main isoform in Ca2+signal modulation via P2Y12. Examination of targets downstream of ACBDFig. 10. PI3-K ß-isoform mediates ... considerable amount of the PIP3formed in plate-lets [21], this may suggest that the PIP3produced bythis isoform leads to plasma membrane binding and, hence, activation of PH domain-containing phospho-lipase ... and not the phosphoinositide3-kinase c isoform is responsible for the prolonged Ca2+response and for the subsequent increases in procoagulant activity and coagulation. Takentogether, these...
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Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans pptx
... masses of the mature form of the subunits (14 472 and 40 421 Da for the small and large subunits, respectively). (The published nucleotide sequence for the aau B gene is incorrect [7]. The predicted ... biogenesis genes from the maugene cluster. By performing detailed kinetic studies of both AADH enzymes, we show that the recombinantenzyme is indistinguishable from the native AADH of A. faecalis and ... suppliers.Growth of cells and media The bacterial strains and plasmids used in this study are lis-ted in Table 6. For strain stocks and DNA isolation, E. coli and A. faecalis were cultured with Luria–Bertani...
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Tài liệu Báo cáo khoa học: Mammalian mitotic centromere-associated kinesin (MCAK) A new molecular target of sulfoquinovosylacylglycerols novel antitumor and immunosuppressive agents pptx
... the direction of walking on micro-tubules. MCAK belongs to the Kin I subfamily and its motor domain, unlike most kinesins, is in the interior of the protein. Moreover, the protein localizes at ... binding. The tightness of binding maydetermine the degree of inhibition.SQAGs inhibit the depolymerization activity of MCAK in vivoTo elucidate the MT-depolymerizing effects of SQAGs in vivo, ... and is found in inclusion bodies. Therefore binding betweenSQAGs and a truncated version (MCAK184) wastested using a Biosensor BIAcore instrument. The MCAK184 contains the neck region and the...
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Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc
... the wild-type level and above.Fig. 1. Glycolysis and the las operon in Lactococcus lactis .The las operon in L. lactisconsists of the three genes pfk, pyk and ldh, coding for phosphofructokinase ... all the genesinvolved in the pathway in the same operon; the cellcan then respond quickly to changes in the environ-ment by changing the expression of only a few genes and using the protein-synthesizing ... expression of the entire lasoperon, and show that the data fit well with the indi-vidual determination of flux control coefficients byPFK, PK and LDH. The role of the las operon is dis-cussed on the...
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Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt
... during intermoult and premoult. The resultsshow that the most important level of moult control lieswithin the signalling machinery of the target tissue, thatexpression and biosynthesis of ... MIH and CHHduring precisely timed stages of the moult cycle of Carcinusto determine changes in: (a) potency of these peptides in repressing ecdysteroid synthesis; (b) receptor density and affinity; ... level. In view of these observations, we also investigated levels of MIH and CHH peptides in the sinus gland and levels of transcripts in XO neurones during the moult cycle, to seewhether significant...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinChuong 2 nhận dạng rui roTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM