Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf

... compilation ª 2009 FEBS Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure Martina Andberg1, Nina Hakulinen2, Sanna Auer1, ... show the critical role of the lastamino acids in the C-terminus of MaL. Deletion of the four last amino acids strongly reduced the expression of the protein in T. reesei and S. cerevisiae. Further-more, ... preventing binding of oxygen.We analyzed the azide inhibition and determined the inhibition constants for sodium azide, in order to see the effect of the mutation (L559A) on the bindingproperties...

Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

... in obese mice In order to gain insights into the potential biologicallyrelevant role of miR-27 in the regulation of adipose tis-sue functions in vivo, we examined the expression of miR-27 in ... together, these results illustratea critical and speciﬁc role of miR-27 in the regulation of adipogenic differentiation.miR-27 prevents the induction of PPARc and C⁄EBPa In order to delineate ... differentiationwas initiated at 48 h post-transfection by maintaining the cells in culture medium containing 2% horse serum. Cells were ﬁxed on day 4 of differentiation and stained with hematoxylin and eosin....

Tài liệu Báo cáo khoa học: Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity docx

... moleculecomprising Ôresidues in the protruding domain consisting of the type VI b-turn and the ﬁrst part of b-strand 3Õ (i.e. the b-hairpin loop and part of the adjoining b-sheet) and Ôresidues in the loop ... obtained by other groupswho also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutationalanalysis [35] and structural analysis of the plant defensinRs-AFP1 ... in which the sequence of the b-strands was maintained but the amino acids from loop 3 had been replaced bymultiple Ser and Gly residues. Therefore, in the sequence of the whole b-hairpin structure of...

Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx

... gene. Therefore, both free pregnenoloneproduction and the efﬁcient coupling of the SCC and the 3b-HSD activities in yeast require the use of strains lackingthis acetylating activity. For this ... targeting to the PM. For example, the mature forms of the human, bovine and pig CYP11A1 have two positively charged residues at theirNH2termini that could favor targeting to the PM [58]. The ... Moreover, the protein is apparently catalytically competent in the yeastPM, based on the observation of the speciﬁc depletion of ergosta-5-eneol in the PM ([19], and this work). Interest-ingly,...

Báo cáo khoa học: Essential roles of lipoyl domains in the activated function and control of pyruvate dehydrogenase kinases and phosphatase isoform 1 pot

... acetyl-transferase-catalyzinginner domain. E3BP domains: L3, N-terminal lipoyl domain; B, E3binding domain; I, inner domain which associates with the innerdomain of E2. Dotted connections indicate binding interactions ... running in opposite direc-tions. The monomer association involves the ATP-usingC-terminal domains interacting near the base of that side of the wedge outside; the N-terminal domains form the ... the ability for interactions to induce activating conformationswithin the kinase active site. The much stronger binding of Fig. 2. Surface residues of the L2 domain of E2 that are required for activating...

Báo cáo khoa học: Functional role of the linker region in puriﬁed human P-glycoprotein pot

... determined using the bicinchoninic acid protein assay kit (Pierce, Rockford,IL, USA) with BSA as the standard.Kinetic parameters for the enzymatic reaction of P-gp For rhodamine 123, colchicine, ... that the linker region maintains the tightness of coupling between the ATP hydrolase reaction and substraterecognition. Inspection of structures of the P-gp homolog, MsbA, suggeststhat linker-cleaved ... containingP-gp, the results could be affected by other proteins. In fact, it was reported that the linker region of P-gpinteracts with other proteins such as RING ﬁngerprotein 2 (RNF2) [25] and...

Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops docx

... orientations in the membrane; the N terminus of the N-terminal domainis extracellular, while the N terminus of the C-terminaldomain is intracellular. In the [st326]2HCT family,TMS I is not part of the ... families and the N- and C-ter-minal halves of ST3 (open bars), between the N-terminal halves of ST3 and the C-terminal halves of ST3 (grey bars), and between the C-terminal halves of ST3 and the ... orientations in the membrane. The N terminus of the N-terminal domain is extracellular, while the N terminus of the C-ter-minal domain is cytoplasmic. The loops between the fourth and ﬁfth trans-membrane...

Báo cáo khoa học: Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription ppt

... quantiﬁed using the Scion Image program. The upper numbers represent the intensity of each band normalized to the band in lane 1. The lower numbers represent the intensity of the bands in lanes 5 and ... for initiation both in vitro (Fig. 3A) and in vivo(Fig. 3B) when the R2 5¢-UTR is either deleted or mutated. In contrast, the in vivo results suggest that when the R25¢-UTR is included in the ... cassette (product indicated by the lower arrow to the left of the autoradiograph). All bands were quantiﬁed using the ScionImage program and the ratio between the upper and lower bands for each assay...

Báo cáo khoa học: N-Glycans of the porcine nematode parasite Ascaris suum are modiﬁed with phosphorylcholine and core fucose residues pot

... that in all cases, the PC is linked through the 6-hydroxyl of GlcNAc.Some PC-containing structures were also putativelymodiﬁed by fucose; thus, the linkage and the position of the fucose in these ... resulting in a loss of productivity.Whereas the large adult roundworms reside in the gut, the larvae hatching from ingested eggs travel from the stomach or small intestine via the liver to the ... N-glycan structuresindeed contain a core a1,3-linked fucose, as found in other invertebrates [37]; in this and other studies[22,38], the RP-HPLC retention times of these difucos-ylated structures...

Báo cáo khoa học: Quantitative modeling of triacylglycerol homeostasis in yeast – metabolic requirement for lipolysis to promote membrane lipid synthesis and cellular growth potx

... point of view, they allow the cell to dispose of excessmetabolites by storing them in an inert form. Whendemand for these metabolites is high, they are read-ily available without the need for ... quantitatively the role of TAGdegradation in growing cells and the metabolic ﬂux of FA. We reconstructed the metabolic pathway of TAGlipolysis in yeast in silico and speciﬁcally addressed the question of ... 2), and promotes the rapid initiation of growth of quiescentcells exposed to glucose-containing media. TAG mobi-lization provides DAG and ⁄ or FA for the synthesis of phospholipids; forcing the...

Xem thêm