Báo cáo khoa học: Heme oxygenase-1 ⁄p21WAF1 mediates peroxisome proliferator-activated receptor-c signaling inhibition of proliferation of rat pulmonary artery smooth muscle cells pot

... Heme oxygenase-1 ⁄p21WAF1 mediates peroxisome proliferator-activated receptor-c signaling inhibition of proliferation of rat pulmonary artery smooth muscle cells Manxiang Li1, ... January2010)doi:10.1111/j.1742-4658.2010.07581.xActivation of peroxisome proliferator-activated receptor (PPAR)-c sup-presses proliferation of rat pulmonary artery smooth muscle cells (PASMCs), and therefore ameliorates the development of pulmonary hypertension ... suppressing PASMC proliferation. Proliferation of PASMCs is a hallmark of pathogenesis of pulmonary hypertension [1,4]. How-ever, the mechanisms responsible for inhibition of PASMC proliferation by...

Báo cáo khoa học: How a lipid mediates tumour suppression Delivered on 29 June 2010 at the 35th FEBS Congress in Gothenburg, Sweden pdf

... cytoki-nesis has offered a third possible explanation for thetumour suppressor activity of this enzyme complex[73]. Inhibition of PI3K-III activity not only causes anincreased proportion of cells undergoing ... metabolized through the activities of a num-ber of substrate-speciﬁc PI kinases and phosphatases,the dysfunctions of which are associated with varioushuman diseases [2]. Of special interest in cancerresearch ... detected at the plasma membrane of cells stimulatedwith insulin or lysophosphatidic acid [38,39]. Becausethis pool of PtdIns3P is generated through the activity of PI3K-II [40], which has so...

Tài liệu Báo cáo khoa học: High-afﬁnity ligand binding by wild-type/mutant heteromeric complexes of the mannose pptx

... side of the dimer. Overall, theanalysis of IGF-II binding in such receptor dimerssuggests that each half of the dimer is capable of binding IGF-II independently of the ligandoccupancy of the ... number of wild-type binding sites avail-able in a mixture of mutant ⁄ wild-type receptors.Tethering to the resin of asymmetric heterodimers sug-gests the importance of anchoring the tail of the ... forpermission to use the 293T cells. We are grateful toMargaret H. Niedenthal of Lilly Research Laborato-ries for providing the IGF-II. We thank BarbaraSwitzer and the University of Nebraska Medical...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... Department of Pharmacy, University of Tromsø, Norway2 Department of Medical Biochemistry, University of Tromsø, Norway3 School of Pharmacy, University of Oslo, Norway4 Department of Tumor Biology, ... number of via-ble cells were observed for both cell lines attached toplastic. However, when the cells were attached tomonomeric 2D collagen, the number of viable cells increased, whereas for cells ... Materials and methods, and 81 lg of pro-tein was added to each well. The amount of S100A4 inthe II-11b cells was 1.2% of that in the pHb-1 cells after 2 min exposure of the ﬁlm and 12% after 5 minexposure...

Tài liệu Báo cáo khoa học: 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5¢-phosphate synthases of fungi and archaea docx

... units of guanylate kinase and 2 units of pyruvate kinase in a totalvolume of 0.5 mL, and were incubated for 30 min at 37 °Cin order to generate the reductase substrate 3 (the addition of guanylate ... structure.Quantitative analysis of the signal integrals revealsrapid consumption of the b anomer and less rapid con-sumption of the a anomer of substrate 3. All data arein line with the hypothesis that the ... of the sub-strate, which is progressively regenerated from thea anomer by spontaneous isomerisation (data notshown). The apparent catalytic rates of the recombi-nant enzymes from C. glabrata...

Tài liệu Báo cáo khoa học: DNA modiﬁcation with cisplatin affects sequence-speciﬁc DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... scale of potential reactivityto cisplatin [6,7]. Expression of the p53 downstreameffectors in cisplatin-treated cells may thus be entirelyunbalanced rather than shifted towards one of theresponse ... fragment caused about 70% inhibition of p53(1–363) binding to either of the two targets. Modi-ﬁcation of the p21 ⁄ pPGM1 mixture resulted inrb-dependent inhibition of p53 binding to the p21 ... and cisplatin concentrations of 2.3,4.6 or 6.9 lm were applied to maintain the cisplatin ⁄ nucleo-tide ratios and thus the levels of global DNA modiﬁcation.The number of cisplatin moieties...

Tài liệu Báo cáo khoa học: The most C-terminal tri-glycine segment within the polyglycine stretch of the pea Toc75 transit peptide plays a critical role for targeting the protein to the chloroplast outer envelope membrane ppt

... low activity of the Plsp1 homolog that isresponsible for full maturation of Toc75 in the peaFig. 1. The biogenesis of pea Toc75. The precursor, intermediate,and mature forms of Toc75 are indicated ... by which the polyglycine stretch mediates envelopetargeting of Toc75 remains unknown.In this report, we extended the analysis of the poly-glycine stretch of the Toc75 transit peptide in order ... min of the reaction. If the above possibilitywere correct, we should see changes in distribution of proteins, especially those from AGA, during the timecourse. As shown in Fig. 3, the ratios of...

Tài liệu Báo cáo khoa học: 1,5-Diamino-2-pentyne is both a substrate and inactivator of plant copper amine oxidases ppt

... mechanism of inactivation of GPAO by DAPY, we investigated the inhibitory potency of the two possible mo no-N-methyl derivatives of DAPY withFig. 9. Mechanism of DAPY oxidation by GPAO. The scheme ... for half of the enzyme to become inactivated in thepresence of saturating concentration of inhibitor.bIn 0.1MBistris/HCl buﬀer, pH 7.0.Fig. 2. Partition ratio plot for inactivation of G PAO ... rates of inactivation of 0.1–0.3 min)1were determined, the apparent KIvalues(half-maximal inactivation) were of the order of 10)5M.DAPY was found to be a mech anism-based inhibitor of...

Tài liệu Báo cáo khoa học: Efﬁcient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt

... that mediates theinsertion of four additional nucleotides into a predetermined position of asubstrate RNA, and thus mimics, at the RNA level, the repair of a shortdeletion mutation; 17% of the ... the insertion of four speciﬁc nucleotides into apredetermined site of an arbitrarily deﬁned substrateRNA as illustrated in Fig. 2A. In the ribozyme–substrate complex, a stretch of four nucleotides ... observed. The ratio of product to initial substrate increased; 9% of substrateRNA was converted to the 44-mer and 5% was leftunchanged (Fig. 4B, lane 4, Fig. 4C). These resultsdemonstrate favourable...

Tài liệu Báo cáo khoa học: "A Mobile Touchable Application for Online Topic Graph Extraction and Exploration of Web Content" ppt

... twotypes of word chains. Each chain consists of longestmatching sequences of words with the same PoSclass, namely noun chains or verb chains, wherean element of a noun chain belongs to one of the ... demonstrated the effectiveness of this hypothe-sis for web–based question answering.Finally, we compute the frequencies of eachchunk, each chunk pair, and each chunk pair dis-tance. The set of ... graph in the ﬁnal step. For-mally, a topic graph T G = (V, E, A) consists of aset V of nodes, a set E of edges, and a set A of nodeactions. Each node v ∈ V represents a chunk andis labeled with...

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