Báo cáo khoa học: Recent insights into cerebral cavernous malformations: a complex jigsaw puzzle under construction doc

Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... coronariaRaka Ghosh, Sibani Chakraborty, Chandana Chakrabarti, Jiban Kanti Dattagupta andSampa BiswasCrystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, ... S, Sundd M, Jagan-nadham MV & Dattagupta JK (1999) Crystallizationand preliminary X-ray analysis of ervatamin B and C,two thiol proteases from Ervatamia coronaria. ActaCrystallogr D 55, ... protease erva-tamin A from Ervatamia coronaria. Acta Crystallogr F61, 562–564.37 Kundu S, Sundd M & Jagannadham MV (2000) Puriﬁ-cation and characterization of a stable cysteine proteaseervatamin...

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... the yeast cytochromebc1 complex based on analysis of single and double deletion mutantslacking supernumerary subunits and cytochromebVincenzo Zara1, Ilaria Palmisano1, Laura Conte1and ... Electro-phoretic analysis of DNA on agarose gels, restrictionendonuclease analysis, ligation of DNA fragments,Table 1. Yeast strains used in this study.Strain Genotype ReferenceW303–1 A (WT) MATa, ade2–1, ... genedeletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21,3329–3330.22. Ito, H., Fukuda, Y., Murata, K. & Kimura, A. (1983) Transfor-mation of intact yeast cells treated with alkali cations....

Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

... experiments revealed that ADP signiﬁ-cantly activates OfODC, whereas ATP was only a weak activator [3]. Although the mechanism of activa-tion by ADP remains to be elucidated, the authorspostulated its ... physiological relevance. To date, morethan 50 oxalotrophic bacteria that are capable of usingoxalate as a carbon and energy source have been iden-tiﬁed [6]. The Swiss-Prot ⁄ TREMBL database includes28 ... CoA transferase [2]. Here, a kinetic assay was established to directly monitorchanges in the UV absorbance of the substrate oxal-yl CoA during catalysis. Oxalyl CoA was synthesized[19] and...

Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot

... City, CA, USA).Coagulation assayAPTT assays were carried out on an automatic coagulationanalyser (Hemostasis Analyzer KC-1; Sigma Diagnostics,St Louis, MO, USA) according to the manufacturer’s ... G & Rosing J (2001) Snake venom activators offactor X: an overview. Haemostasis 31, 225–233.8 Takeya H, Nishida S, Miyata T, Kawada S, Saisaka Y,Morita T & Iwanaga S (1992) Coagulation ... the heavy chain, thesense primer was 5¢-GCCAAAT(C/T)CAGCCTCCAAAATG-3¢ and the antisense primer was 5¢-CTGAGAGAAGCCAGTGGTTGA-3¢. To clone its far 3¢ noncodingregion, the sense primer (a 20-mer...

Báo cáo khoa học: New insights into the P-glycoprotein-mediated efﬂuxes of rhodamines doc

... compounds,the parameter k a was calculated. As shown in the MaterialsTable 2. Parameters characteristic of the interaction of rhodamines withcells. The data are the means ± SEM of three determinations. ... substratetransport, is a powerful approach to enhancing ourunderstanding of their function and mechanism. In thispaper, we present data that further characterize thetransport of several rhodamine analogues. ... [9].2-[4-(Diphenylmethyl)-1-piperazinyl]ethyl-5-(trans-4,6-dimethyl-1,3,2-dioxaphosphorinan-2-yl)-2,6-dimethyl-4-(3-nitrophenyl)-3-pyridinecarboxylate P oxide (PAK-104P)was a gift from Drs N. Shudo, T. Iwasaki and S.I. Akiyama, Nissan, Chemical Industries, Ltd. All the reagents were ofthe highest quality available and deionized...

Báo cáo khoa học: The male seahorse synthesizes and secretes a novel C-type lectin into the brood pouch during early pregnancy pdf

... sterile water.The cDNA was ligated into the Uni-ZAP XR vector(Stratagene) using T4 DNA ligase at 4 °C for 2 days andthen packaged into Gigapack III Gold Packaging Extract(Stratagene) by incubating ... Chemical Co.) for 1–5 min.Bacteriostatic assaysBacteriostatic assays were carried out essentially as inBiswas et al. [28]. LB media (1 mL) was inoculated withDH 5a E. coli and incubated at 37 ... Chitinase3 [P. olivaceus] (4e-13) CV864030WQ114 ATPase subunit 8 (ATPase8) andATPase subunit 6 (ATPase6) – mito-chondrial [Rhamdia laticauda] (4e-17)ATP synthase F0 subunit 6 [P. olivaceus]...

Báo cáo khoa học: Mathematical modelling of the urea cycle A numerical investigation into substrate channelling docx

... k3;ASSk5;ASSk7;ASSk9;ASSk11;ASSATP(t) Asp(t) þ k2;ASSk5;ASSk7;ASSk10;ASSk12;ASSAsp(t) AMP(t) (as(t)þ asR(t)) + k1;ASSk3;ASSk5;ASS(k7;ASSk9;ASSþ k7;ASSk11;ASS+ k9;ASSk11;ASS) ... The construction of detailed, andnecessarily complex, mathematical models serves as a ÔtoolÕto facilitate analysis of channelling in biochemical pathwayslike the urea cycle.There is a range ... a grant from the Australian NationalHealth and Medical Research Council and the Australian ResearchCouncil to P. W. Kuchel. A. D. Maher is the recipient of a University ofSydney Postgraduate...

Báo cáo khoa học: "GETTING IDIOMS INTO A LEXICON BASED PARSERS HEAD" pptx

... derive an overall measure of likelihood of a partial analysis. Measures are included for the likelihood of that particular reading of the word and for aspects attached to an impulse: a) for ... ~xical tasks, extension tasks, insertion tasks and virtual tasks. A lexical task specifies 53 a possible reading era word to be introduced in the chart as an inactive edge. An extension task ... weight (for instance the new alternative may be a partial NP instead of a complete NP (as in take care), or a NP marked differently from usual). The latter specifies that a certain impulse, specified...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... Canyuk B, Focia PJ & Eakin AE (2001) The role foran invariant aspartic acid in hypoxanthine phospho-ribosyltransferases is examined using saturation muta-genesis, functional analysis, and ... 6615–6627.24 Raman J, Sumathy K, Anand RP & Balaram H (2004) A non-active site mutation in human hypoxanthineguanine phosphoribosyltransferase expands substratespeciﬁcity. Arch Biochem Biophys ... ﬁlter paper assay and tritium-labeledsubstrates. Experiments have been repeated three to four times and the data are given as the mean ± SD. kcatwas calculated usingMw(HPRT) = 27132 Da and...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx

... regulation and mixed lineageleukemia.AcknowledgementsWe thank Imran Hussain, Sahba Kasiri, BishakhaShrestha, Saoni Mandal and other Mandal lab mem-bers for useful discussion and critical comments. ... geneactivation A widely studied NR coactivator is activating signalcointegrator-2 (ASC2, also named AIB3, TRBP,TRAP250, NRC, NCOA6 and PRIP) [69]. ASC2 is a coactivator of multiple nuclear receptors ... actas a histone methylase during basal transcriptionwhereas MLL2, MLL3 and MLL4 replace MLL1during activated transcription and act as coactivators,at least in selected HOX genes.MLLs contain...

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