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Báo cáo khoa học: Recent insights into cerebral cavernous malformations: animal models of CCM and the human phenotype pptx

Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... erva-tamin-A, the P3 moiety of both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the solvent(Fig. 2B). On the other hand, the P3 ... at the bottom of the subsite is conservedin ervatamins. Compared to a Ser in the equivalentposition of papain, the role of Leu here is to restrict the size and depth of the S2 subsite. The ... proton from the catalytic Cys pres-ent at the N-terminus of the helix to the His of the dyad [19,50,51]. The first stage of catalysis is mediatedby the highly active thiolate ion of the Cys. Biochemi-cal...
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Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

Tài liệu Báo cáo khóa học: Further insights into the assembly of the yeast cytochrome bc1 complex based on analysis of single and double deletion mutants lacking supernumerary subunits and cytochrome b pdf

... 7. The arrowslabeled (b) and (c) point to the areas of interaction between the transmembrane helix of subunit 8 and helices G and H1 of cytochrome b and between the N-terminus of subunit 8 and ... panels A and B and the relative amounts of each of the subunits determined by densitometry scanning of the stained Westernblots of the and CKL57 membranes are shown in panels C and D, respectively.Ó ... inability of the truncated protein to insert into and be stable in the inner mitochondrial membrane or lack of detection of the truncated protein by the antibodies.In the W303–1B q° strain the amounts...
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Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx

... The mainchain nitrogens of residues I322 and I303 interact withnitrogen atoms of the adenine ring and the c-carboxylgroup of the side chain of residue D302, the d and xnitrogen atoms of the ... tohomogeneity. The kinetic constants for conversion of the substrate oxalylcoenzyme A by the enzyme in the absence and presence of the inhibitorcoenzyme A, as well as in the absence and presence of the ... on the ratio between the I(0) of EcODC and that of BSA(4 mgÆmL)1) and the molecular mass of the latter (67 kDa).Theoretical scattering patterns were calculated from the crystal structure models...
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Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot

Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot

... plate-lets and endothelial cells. The important role of theseglycans in pharmacokinetics has been demonstrated by the slower and smaller increment of FDP in vivo after the injection of desialylated ... analyses of the glycan epitopesSamples of 7 lg of RVV-X and 5 lg of BSA were analysedby 8% SDS-PAGE under reducing conditions. Appropriateamounts of Lex/a- and SLex/a-conjugated BSAs and human serum ... sequenced. Of these, 10clones were found to encode the LC2 and LC1 sub-units. Others were found to encode other variants of the C-lectin-like venom proteins. The amino acidsequences of both subunits...
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Báo cáo khoa học: New insights into the P-glycoprotein-mediated effluxes of rhodamines doc

Báo cáo khoa học: New insights into the P-glycoprotein-mediated effluxes of rhodamines doc

... the cytosol. This observation allows the further determination of the concentration of drug free in the cytosol of resistantcells. The determination of karequires also the measurement of the ... Na+-buffer on the one hand and K+bufferon the other hand. In Na+-buffer a strong decrease of the fluorescence signal was observed due to the accumula-tion of the lipophilic cation mainly in the mitochondrialcompartments, ... presence of the pump, the free intracellularrhodamine concentration, the rate of their passive diffusionthrough the plasma membrane and then the kineticparameters characteristic of their P-gp-mediated...
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Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx

Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx

... observation of the protein’sinfluence on the progression of the cell cycle and the silica biogenesis. As the regulation of NR expressionappears to be very similar throughout the diatomrealm [12,13], the ... genes.Furthermore, the addition of both nitrate and appro-priate concentrations of ammonium allows, within lim-its, the control of the amount of induced protein. The observed lag phase of 1 h for Pnr ... demonstrated that the 5¢-UTR and 3¢-UTR of CfNR allow control of both the timing and level of expression of introducedgenes in transgenic C. fusiformis.ResultsIncreasing the efficiency of C. fusiformistransformationPreviously,...
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Báo cáo khoa học:

Báo cáo khoa học: "A Word-Order Database for Testing Computational Models of Language Acquisition" docx

... inversion, etc. The LDD on the web: The two primary purposes of the web-interface are to allow the user to interactively peruse the patterns and the derivations that the LDD contains and to download ... outcome and do not have access to the on-line operations of the parser. Originally, the ability to detect ambiguity was employed in two variations of the STL: the strong STL (SSTL) and the weak ... settings and annotations are saved and available the next time the user logs on. Finally on the Data Download page, users may download data so that they can use the patterns and derivations offline....
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Báo cáo khoa học:

Báo cáo khoa học: "GETTING IDIOMS INTO A LEXICON BASED PARSERS HEAD" pptx

... Substitutions include the semantics of the idiom, which are supposed to take the place of the literal semantics, plus the specfication of the new Main and of the bindings for the functions. New ... form of the verb. The fwst portion of the representation is devoted to the literal interpretation of the word, and includes the semantic representation, the l/kelihood of that reading, and ... an expected fragment of an idiom in the table raises the level of activation of that idiom, in proportion to the relative weight of the fragment. If the configuration of the sentence contrasts...
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Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane affinity and hyper-anticoagulant activity in mouse plasma pptx

Tài liệu Báo cáo khoa học: Mouse recombinant protein C variants with enhanced membrane affinity and hyper-anticoagulant activity in mouse plasma pptx

... of bovine prothrombin fragment 1in complex with Ca2+[41], the 10 ⁄ 12 site resides near the x-loop and the linear array of bound calcium ions of the Gla domain. Indeed, the importance of the 10 ... evaluated [11]. Of the Gla residues, nine are strictly conserved through-out the Gla proteins. From crystal structures of the Gla domain of prothrombin and factor VIIa, the placement of the seven ... inside out, exposing the hydrophobic x-loopto solvent and burying the majority of the Gla resi-dues. Of the seven Ca2+, the majority are buried and are integral to maintain the membrane-binding...
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Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

... in terms of its ability toenter the cells of the retina, the present study used reti-nal pigment epithelial (RPE) cells as a model to deter-mine the susceptibility and the mediators of HSV-1entry ... post-transfection, the target and the effector cells were mixed together (1 : 1 ratio) and cocultivated in 24-well dishes. The activation of the reporter luciferase gene as a measure of cell fusion ... of the viral envelope with the plasma membrane of host cells [7]. Subsequently,viral capsids and tegument proteins are released into the cytoplasm of the host cell. The gD receptors include cell...
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