Báo cáo khoa học: Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein doc

... 5¢-ATC CGG GGT CTCCCA-TG GCA ATG AGG GCT GCG GGT G-3¢; HCV-1bCore+1/S sense, 5¢-ATC CGG GGT CTCCCATG GCAATG AGG GCC TGG GGT G-3¢; HCV-1a Core+1/Santisense, 5¢-AT CCG GGT CTCGGTACC TTA TCACGC ... reading frame protein; DLS, dynamic light scattering; HCC, hepatocellular carcinoma; HCV, hepatitis C virus; HSQC,heteronuclear single quantum coherence; IDP, intrinsically disordered protein; ... of infection, with the possible develop-ment of hepatocellular carcinoma (HCC) in 1–4% of cases [3]. No prophylactic vaccine against HCV exists,and the efﬁciency of therapies is hindered by the extreme...

Báo cáo khoa học: Abundance of intrinsic disorder in SV-IV, a multifunctional androgen-dependent protein secreted from rat seminal vesicle pot

... also convergedto indicate an abundance of intrinsic disorder in SV-IV, but few amino acids in the C- terminal region. In particular, hydrophobic clusters, which are typical of secondary structure ... recognitionaccuracy can be obtained by an SVM that incorporatesonly amino acid composition, and very good recogni-tion accuracy was retained using reduced sets of aminoacids based on chemical ... biological function,and should therefore be better classiﬁed as an intrinsically disordered protein. AbbreviationsHCA, hydrophobic cluster analysis; IDPs, intrinsically disordered proteins; PDB, protein...

Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

... the expression of activating transcription factor 4 (ATF4)and of CCAAT⁄ enhancer-binding protein homologous protein (CHOP) proteins downstream of eIF2a wereobserved (Fig. 4A), indicating the initiation of ... of a variety of celltypes, including hepatocytes, to long-chain saturatedfatty acids (SFAs) led to increased expression of proin-ﬂammatory cytokines, inhibition of insulin signaling,induction ... metabolism of FFAs aftertheir uptake into liver cells is the long-chain acyl-CoAsynthetase (ACS), which activates fatty acids by link-ing them to coenzyme A. As shown in Fig. 6, triac-sin C (TrC),...

Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

... was also accompaniedby a decrease in the Trp fluorescence intensity. Plotting the percentage of initial fluorescence as a function of the lipid concentration (Fig. 2) enabled calculation of Kdvalues. ... wavelength of 290 nm, at a slit width of 4 nm. The fluorescence spectrawere instrument corrected for light scattering, by subtract-ing the corresponding spectra of the SUVs.Changes in Trp fluorescence ... electrostatic interactions play a principal role in the binding of E2(7–26) to negatively charged residues. In contrast, a higher contribution of hydrophobic com-pared with electrostatic interactions...

Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf

... be binding sites for the Orc1 ⁄ Cdc6 proteins. The oriC1 origin is located upstream of the cdc6-1gene, encoding the Sulfolobus ortholog of the Pyro-coccus Orc⁄ Cdc6 and Halobacterium Orc7 proteins.Moreover, ... exam-ple, the positioning of nucleosomes at an origin can be in uenced by the binding of ORC [29]. Additionally, the acetylation status of chromatin can in uence tim-ing of origin firing in budding ... Subsequentwork confirmed the position of the origin of replica-tion in Pyrococcus, providing the first experimentalproof of a localized origin of replication in the archaea[52]. Interestingly, in a situation...

Báo cáo khoa học: Enzymes of mannitol metabolism in the human pathogenic fungusAspergillus fumigatus– kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications pot

... provides the residues forbinding the coenzyme. The C- terminal domain has a lar-gely a-helical structure, and serves mainly in substratebinding. The active site is located in the interdomaincleft, ... and so the choice of the constantlevel of Fru was not critical for determination of DKm(1.23 ± 0.15). The KIE data in Table 4 are instrumen-tal in delineating the kinetic mechanism of AfM1PDHand ... binding ﬁrst is a clear requirement of the strictly ordered kinetic mechanism, because, at satu-rating concentrations of the substrate which is addedsecond, the commitment to catalysis becomes...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... [24]. Interestingly, phos-phorylation of CYP1A1 ⁄ 1A2 occurred at 24 h afterSham GdCl3CLP GdCl3 + CLPCYP1A1β-actinβ-actinβ-actinβ-actinCYP2B1CYP2E1CYP1A2Fig. 4. Effects of KCs on ... metabolic activation of numerous procarcino-gens, including aﬂatoxin B1 [21]. In the present study,CYP1A1 and 1A2 activities were signiﬁcantlydecreased, with a concomitant decrease in their protein levels ... vulner-able to GdCl3because only some of the red pulp mac-rophages transiently disappear. The white pulp Sham GdCl3CLP GdCl3 + CLPCYP1A1β-actinβ-actinβ-actinβ-actinCYP2B1CYP2E1CYP1A2Fig....

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... (2001) Assembly of scaffold-mediated complexescontaining Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phos-phorylation of Cdc24p. J Biol Chem 276, ... is consistent with the genetic interactions reported previously. The dele-tion of either ERG4 or NCP1 in the cla4D backgroundis lethal, indicating that CLA4 and these genes, encod-ing proteins ... total of 105cells of the indicated plasmidcombinations were spotted either onto medium lacking histidineand leucine to select for the plasmids, or onto medium lacking histi-dine, leucine and...

Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... nuclear extracts from SP1 cells. The nucleo-tide sequences of putative CERK-PPRE, including the sense 5¢-CTCTCCAGGCCACAGGCCAGAGCGG-3¢ andanti-sense 5¢-GAGAGGTCCGGTGTCCGGTCTCGCC-3¢sequences, ... to sphingosine and thenrecycled into intracellular Cer itself, and that accumu-lation of Cer contributes to the induction of apoptosis[20]. However, the signaling mechanism involved in Cer-mediated ... 5¢-CTGCCCAAGGGTGCTGTGCTC-3¢ and for reverse 5¢-TTGTTCTCACCAGACCCTTGAC-3¢. All reactions were run witha hot-start preincubation step of 10 min at 95 C, followedby cycles of 15 s at 95 C and 1 min at 60 C. The...

Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitriﬁcans pptx

... tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalisCofactor assembly and catalytic properties of recombinant enzymeexpressed in Paracoccus denitrificansParvinder ... activator of the mauF promoter were amplified from genomic DNAusing forward (5¢ -TTGGTAAGCTTGGGCATTTCTGATCGGGTCGC-3¢) and reverse (5¢-AAACCATATGACGCCTCCTCTCGCT-3¢) primers that incorporated HindIII ... the 5¢-end of the amplification product, and an XhoI site in the 3¢-end. The amplification product was cloned into the TAcloning vector PCR4-TOPO (Invitrogen, Carlsbad, CA) forsequencing and then subcloned...

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