Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf
Báo cáo khoa học: Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases pdf
... andC-terminal domain, including the highly conservedGSH -binding site (G -site) and the more divergentcosubstrate -binding domain or hydrophobic binding site (H -site) [7,18]. The G -site includes both a-helicesand ... Different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione S-transferases Angela R. Lo Piero, Valeria Mercurio, Ivana Puglisi and Goffredo ... structure elements. The topological arrangement of these elements is usuallybababba, similar to the thioredoxin fold of otherGSH -binding or cysteine -binding proteins. The H -site is entirely helical,...
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Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc
... GATA-transcription factor binding site in the c-T2 promoter, although other processes might also beinvolved. In multicellular organisms, GATA -binding factors play critical roles in development, includingcell-fate ... participates in the assembly of the mitotic spindle of E. focardii and that both isotypes are involved in the nucleation of other microtubule structures.Finally, we examined the distribution of E. ... formation of the GTP -binding site. The alanine at280 in c-T1 apparently causes a conformational change in the M loop (S7-H9), which may in uence lateralinteractions (Fig. 4A, double arrow). The substitutionsFig....
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Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx
... homologues in the context of DNA binding and chromatin regulation. These homologues includemembers of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated CpGislands ... inhi-bitor of growth protein 3 (ING3) PHD, which isanother domain involved in chromatin binding andclearly more divergent (e-value 0.019) from DIDO and the other sequences. As the alignment ... from the other bran-ches, which was indeed predicted. The other maingroup, obtained 97% of the time in 20 740 exploredtrees, contained the DIDO PHD sequences clusteredwith the PHF and the...
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Báo cáo khoa học: Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor ppt
... fitting procedure to determine total andnonspecific binding at the same time. In saturation binding experiments the experimentally determined nonspecific binding was used to estimate nonspecific binding ... [28]. The N-terminal sequence of the H1 receptor indeed contains two N-linked glycosylation consensus sites, but in prelim-inary studies no binding to concanavalin A was observed,indicating that ... of GPCRs can occur without loss of ligand binding capacity, if the seven transmembrane segments harboring the binding site can functionally interact without connecting loops[42,43, (W. J. DeGrip,...
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Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx
... promoting an increase in proopiomelanocortin and cocaine and amphetamineregulated transcript. Taken together, these findingsprovide a compelling argument for the role for malo-nyl-CoA in regulating ... needs to beexplored further. Other areas of the brain have alsobeen shown to regulate feeding via AMPK [35,36]. Of interest is the extensive use of fructose as asweetener in the human diet [37]. ... and certain amino acids [16].A linkage between fatty acid synthesis in the CNSand feeding behavior was uncovered with the findingthat systemic or intracerebroventricular administration of fatty...
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Báo cáo khoa học: A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components ppt
... following exposure to calcium iono-phore, indicating that they were correctly targeted into secretory granuleseven in the absence of serglycin. In the absence of serglycin, the fates of the serglycin-dependent ... on serglycin. Together,these findings indicate that the reduced amounts of neutral proteases in the absence of serglycin is not caused by missorting into the constitutive path-way of secretion, ... explan-ation for these findings would be that the absence of SG causes missorting of mMCP-6 and pro-CPA into the constitutive rather than into the regulated pathway of secretion. If indeed this were the case,...
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Báo cáo khoa học: Calcium, mitochondria and oxidative stress in neuronal pathology Novel aspects of an enduring theme pdf
... increase in the amount of ROS [21]; (b) loss of cytochrome c impairs the flow of electrons in the respiratory chain inducing over-reduction of the complexes, favouring the generation of ROS [16,17,22]; ... element binding protein, iso-citrate dehydrogenase is an RNA -binding protein, whilekgd2p is a mitochondrial DNA binding protein [81–84].Mitochondria from different brain regions contain different ... upon which the cell looses the ability to buffer the incoming calciumresulting in an abrupt, sustained and irreversibleincrease in [Ca2+]c, probably coincides with the clea-vage of the exchanger...
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Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf
... concentrations. The d2-crystallin in lens was recruited during evolutionfrom argininosuccinate lyase, an enzyme involved in arginine biosynthesis derived from the urea cycle. These two proteins shared ... stabilityfor crystallins is essential in maintaining lens transparency[4]. Determining the mechanism of folding and assembly of these proteins is important for understanding how they canform stable ... further increased. The unfolding curveusing the ANS fluorescence probe suggested a multistateprocess, consistent with the results obtained by proteinintrinsic fluorescence and CD. The binding...
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Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx
... chain is brefeldin A-insensitive and isinhibited by the proteasome inhibitor clasto-lactacystinb-lactone, resulting in the accumulation of ricinA chains. These stabilized ricin A chains are partlydeglycosylated ... Threehigh-lysine mutations control the level of ATP -binding HSP70-like proteins in the maize endosperm. PlantCell 3, 507–515.69 Zhang F & Boston RS (1992) Increases in binding protein (BiP) ... pivotal role of the UPR is to maintain ER home-ostasis. Therefore, the presence of mutated proteinsthat are unable to fold into their native conformation in the ER induces the UPR in an effort...
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Báo cáo khoa học: A di-leucine sorting signal in ZIP1 (SLC39A1) mediates endocytosis of the protein doc
... detec-ted in the perinuclear region of the CHO cells with apunctate distribution in the cytoplasm of the cell(Fig. 2A). The perinuclear staining of the wt ZIP1-Mycprotein was strikingly coincident ... the di-leucine signal of ZIP1 and substitutions of the di-alanine residues for the di-leucine residues in the ZIP1 sorting signalinhibited the internalization of the chimeric protein.DiscussionIt ... sorting sig-nal(s) in ZIP1 were first sought by examining the functional sites predicted in ZIP1 using the ELM ser-ver (the eukaryotic linear motif resource for functional sites in proteins;...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíTổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ