... Small peptides derived from the Lys active fragment of the
mung bean trypsin inhibitor are fully active against trypsin
Rui-Feng Qi
1
, Zhi-Xue Liu
2
, ... studied
Bowman–Birk protease inhibitors. A modified peptide, Lys3 3GP, with 33
residues derived from the long chain of the Lys active fragment of mung
bean trypsin inhibitor, w...
... constitute the smallest
fragment (p16) of p35 that fully activates Cdk5 [20]
(Fig 1A). That study found that further truncation of p16,
removing either the N-terminal 11 residues (part of the
p35 ... appears to be a
consequence of the rigidity of the cyclin box structure of the
activator compared to the relatively more flexible T-loop.
Judging from the crystal...
... A common drawback of such approaches
is the cost of the affinity resins used to isolate the
fusion protein and the proteases required to liberate
the protein of interest from the fusion protein. ... concentrated manner
at the top of the column, or, if bound to the resin
prior to packing the column, the salt gradient concen-
trates the peptide on its way out o...
... when the CABS charged cluster or the LBD
are mutated are not simply due to decreased protein
expression or stability. Neither mutation of the CABS
VH2-C domain charged cluster nor deletion of the
LBD ... pro-
teins, but we expect that the relative expression level of
the tagged proteins is indicative of that of the equival-
ent untagged proteins.
To assess the fu...
... antibody raised against KLK11 was the same size
as that of the translational product by isoform 2
mRNA (Fig. 4B). The product by isoform 1 mRNA
was smaller than the isoform 2 product. The multiple
bands ... isoforms 1 and 2 are
detected in the organ. All of these nucleotide sequences
were identical to the genomic sequence of the human
KLK11 gene (GenBank accession nu...
... mediates activation
of Ras subsequent to the stimulation of G
i
- and G
q
-
coupled receptors [8,9].
The main mechanism for the activation of Ras-
GRF1 is the binding of Ca
2+
⁄ CaM to the N-terminal
IQ ... identified [17,19]. The smaller iso-
forms correspond to N-terminal deletions of the full-
length 140 kDa protein. The physiological role of the
guanine nucleo...
... the scale of the Bard’s genera-
tivity, and the quality of its insights.
5 Empirical Evaluation
The vastness of the web, captured in the large-
scale sample that is the Google ngrams, means the
Jigsaw ... properties are indeed acquired
from the Web for our vocabulary of stereotypes
(this point relates to the aptness of the similes sug-
gested by the Bard)...
... coreceptors, are m ainly
regulated at the transcriptional level. This report s hows that
the b FGF r egulation of their expression speci®cally depends
on the nature of HSPG and of the Sertoli ... (HSPG) on the cell surface [11].
Oligosaccharidic sequences of HS chains are de®ned for the
bFGF binding and for the recognition of the speci®c bFGF
receptor, leading to...
... regulates the formation of transport complexes.
GTP hydrolysis by Ran in the cytoplasm drives disso-
ciation of the export cargo from the export complex,
whereas RanGTP promotes disassembly of the ... regulates the move-
ment of molecules into and out of the nucleus and is
necessary for the survival of eukaryotic cells. The pas-
sage of molecules such as RNA,...
... linkers
The initial analysis of 40 amino acid sequences of GH 13
members having the ÔclassicalÕ SBD (Table 1) revealed that,
in fact, there are two groups of these enzymes, which are the
five-domain ... agreement
with the complete-sequence tree (Fig. 3A). In the group of
CGTases produced by the genera Bacillus and Thermo-
anaerobacter (the large compact clusters in th...