Báo cáo khoa học: Regulation of matrix metalloproteinase activity in health and disease pdf

... FEBS 45MINIREVIEW Regulation of matrix metalloproteinase activity in health and disease Elin Hadler-Olsen, Bodil Fadnes, Ingebrigt Sylte, Lars Uhlin-Hansen and Jan-Olof WinbergDepartment of Medical ... (2005) Dissecting therole of matrix metalloproteinases (MMP) and integrinalpha(v)beta3 in angiogenesis in vitro: absence of hemopexin C domain bioactivity, but membrane-Type1-MMP and alpha(v)beta3 ... processing of one or several nuclear proteins, whereas the other isindependent of the processing capacity of the protein-ase and involves the HPX domain. A DNA-bindingdomain was found in the...

Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

... elec-trophoresis. Increasing the concentration of 2A2 mAbat a ﬁxed molar ratio of cathepsin B and cystatin Cresulted in weaker bands of the cathepsin B ⁄ cystatin Ccomplex and stronger bands corresponding ... other cysteine proteases(e.g. papain and cathepsin L) [31]. Removal of theoccluding loop contacts results in a dramatic increase in endopeptidase activity, suggesting that the binding of endopeptidase ... al. [30] and was found to be 2.7 ± 1.8 nm,depicting a strong interaction between 2A2 mAb and cathepsin B.Determination of the 2A2 mAb binding site oncathepsin BThe binding site of 2A2 mAb...

Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx

... section and scheme of the coronal section at E14.5 in (F). (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline. Surprisingly, the ... HSF4-bindingregions and of the transcriptional status of geneslocated around the HSF4-binding regions. This resultis thus suggestive of a structural effect of HSF4 onchromatin. In the absence of ... and inclusion-likestructures, which possibly exist in protein aggregatesbecause they contain aB-crystallin. More than 90% of the lens protein is composed of a variety of crystallins;Role of...

Tài liệu Báo cáo khoa học: "TREATMENT OF LONG DISTANCE DEPENDENCIES IN LFG AND TAG: FUNCTIONAL UNCERTAINTY IN LFG IS A COROLLARY IN TAG" ppt

... define the operation of adjoining. Consider the adjoining of/ ~ at the node marked with * in a. The subtree of a under the node marked with * is excised, and/ 3 is inserted in its place. Finally, ... expressions used in LFG and the elementary trees of TAG. I.I OUTLINE OF THE PAPER In Section 2, we will define briefly the TAG for- malism, describing some of the key points of the linguistic theory ... the adjoining sequence at the root is inde- pendent of other nodes in the tree in spite of the coindexing. This is due to the fact that as the fea- ture structure of the foot of/ ~1 gets instantiated...

Báo cáo khoa học: Optimization of P1–P3 groups in symmetric and asymmetric HIV-1 protease inhibitors pptx

... property of not only the compounds in thisseries but also of other linear inhibitors [44]. The positioning of the inhibitor in the active site results in a tight interactionbetween the inhibitor’s ... A˚on one of thesides leading to loss of the interaction with O of Gly127 butinstead making contact with C and O of Gly149.Optimization of the P2/P2¢ side chainsThe chemical properties of the ... by contributing additional bonds to the protein–inhibitor complex [52–54]. In support of this role of watermolecules in enzyme–ligand complexes is the ﬁnding thatcompounds 2 and 5 inhibit the...

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... release of cytochrome c from mitochon-dria into the cytosol through inactivation with Bcl-xL and Bcl-2 [69]. Inactivated Bax and Bad bind 14-3-3,the phosphoserine ⁄ threonine binding proteins in ... exist in living cells and casp-ases are indispensable for the proliferation of some celllines. When deciding between maintaining cells alive orinducing apoptosis, caspase function and activationare ... cleavage fragments of caspase-3 ⁄ 7sometimes exist in living cells, but are inactive due tothe binding of XIAP [50]. Although chemical caspaseinhibitors bind to caspase fragments and inhibit theirpeptide-speciﬁc...

Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

... that induced by heatstress and induces HSF1 DNA-binding and transcrip-tional activity [68].To induce transcription, direct interactions betweenHSF1 and components of the transcriptional machin-ery ... [91–93]. Recently, Cullin3, a subunit of a Cullin-RING E3 ubiquitin ligase, was reported tointeract with the enriched in proline, glutamate, serine and threonine (PEST) sequence of HSF2, which coulddirect ... during mitosis and to enhance the DNA-bindingcapacity of HSF2 [96,98]. However, another reportshowed that the modiﬁcation rather hinders the DNA-binding activity of HSF2, without interfering...

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... designated A and B,originating from the A and B chains in the structure.dTTP binds at the site of the protein shown previouslyto bind the nucleotides dUTP and dCTP in wild-type and the E138A ... substrate and inhibitor for binding to thesame site [12]. We have previously determined thestructures of wild-type dCTP deaminase in complexwith dUTP and the inactive E138A mutant protein in complex ... of dCTP binding by dTTP. However, thepresence of dTTP in the assay incubation alsoincreased the apparent cooperativity of dCTP bind-ing, which indicates that the mechanism of dTTPinhibition...

Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

... ed-end actin and determine thelevel and distr ibution o f F -actin [34]. Therefore, it i stempting to speculate that interactions between monomericG-actin and actin-binding proteins are a ... fixed, permeabilized and stained for F-actin with rhodamine-conju-gated phalloidin. Th e effects of toxin B and latrunculin B on the actin filaments wasexamined by preincubating the cells witheither ... s pecifically and e ffectivelyinduced maximal inhibition of Erk1/2, p38, JNK1/2 and PtdIns 3-kinase [18,30]. The incubation was further contin-ued in the presence of S1P, anisomycin or serum...

Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc

... published on PEVK domain-containing titin frag-ments (see Introduction for references). The PEVK domainremains the main actin-binding candidate identiﬁed in theI-band titin region and we propose, ... entire length of thin ﬁlaments and iscapable of modulating the r ate of formation of the a ctin–myosin complex [74,75]. Interestingly, nebulin also interactswith the titin PEVK domain in a calcium/calmodulin ... M protein,C protein, MURF-1, calpain 3, myomesin, a-actinin,nebulin, telethonin and obscu rin.The elastic domains ar e made o f t andemly arrangedimmunoglobulin ( Ig)-like domains and a unique...

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