Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx

... kineticparameters Km, Vmax and kcatwere determined for thepuriﬁed AATB3. Values for Km and Vmaxfor bothamino donors (l -aspartate and l-glutamate) and ac-ceptors (a- ketoglutarate and oxaloacetate) ... L-glutamate.The activity of L -aspartate was adjusted to 100.b30 mML -aspartate was used as amino donor for a- ketoglutarate, and 30 mML-gluta-mate was used as amino donor for oxaloacetate. The activity ... aspartate aminotransferase and its complex with maleate.Biochemistry 38, 2413–2424.11 Kim H, Nakaoka M, Yagi M, Ashida H, Hamada K,Shibata H & Sawa Y (2003) Cloning, structural analysis and expression...

Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobiﬁda fusca pptx

... equation.All reducing sugar assays included a glucose standardcurve. The average molecular mass of the XG oligosaccha-rides (XGOs) was calculated from the manufacturer’s data and was found ... Cloning, expression and characterization of a family-74xyloglucanase from Thermobiﬁda fuscaDiana C. Irwin, Mark Cheng*, Bosong Xiang†, Jocelyn K. C. Rose‡ and David B. WilsonDepartment of ... glucose as a standard. Each data point represents the average of threeseparate digestions and the PAHBAH measurements foreach digestion were run in triplicate.Tomato cell walls were isolated as...

Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot

... sulfate fraction; lane 4, recombinant NaoA afterSephadex G75 chromatography; lane 5, puriﬁed recombinant NaoAafter DEAE-Sepharose Fast Flow chromatography; lane 6, standardmolecular mass markers ... b-lactoglobulin A, pI 5.1; myoglobin, pI6.8/7.2; trypsinogen, pI 9.3. The pI of NaoA was determined from a standard curve of pI and migration distance (cm) of protein standards.Enzyme assay and analytical ... completeDNA fragment can catalyze the oxidation of nitroalkanes.In this paper, we describe the cloning and characterization of a novel gene (naoA) that encodes nitroalkane-oxidizingenzyme in S. ansochromogenes.MATERIALS...

Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

... 5¢-GGTTATCATATAAAGATCTCAAATTACCC-3¢, for the second PCRthe primers were: 5¢ primer, 5¢-GGGTAATTTGAGATCTTTATATGATAACC-3¢ and 3¢ primer, 5¢-CGCGCGGGATCCTTAGTGATGGTGATGGTGATGGGTGACCGGTTTTTTGGTAGGTGAAC-3¢.ThethirdPCRwascarried ... proliferation assayWe next analyzed the ability of recombinant SSA tostimulate human T-cells. All SSA preparations yieldedFig. 1. SDS/PAGE and immunoblotting analysis of SSA. (A) 12.5%SDS/PAGE of ... of radio-activity was then measured using a Liquid ScintillationAnalyzer 1600 TR (Packard, Canberra, Australia). Allmeasurements were made in triplicate.Binding analysisThe interaction of...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crabParatelphusa jacquemontiiMaghil Denis, P. D. Mercy Palatty, N. Renuka Bai and S. Jeya SuriyaDepartment ... sialidasetypeX,proteaseenzymes and molecular mass standards were purchased from Sigma.Preparation of crab seraFreshwater field crabs, Paratelphusa jacquemontii werecollected from the local ... Denmark.47. Kamiya, H. & Ogata, K. (1982) Hemagglutinins in the acornbarnacle Balanus (Megabalanus roseus): purification and char-acterization. Nippon Suisan Gokkaishi 48, 1427.48. Kamiya,...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a membrane-bound enzyme complex from the sulfate-reducing archaeon Archaeoglobus fulgidus related to heterodisulﬁde reductase from methanogenic archaea pdf

... with anapparent molecular mass of 53 kDa appears as a double band inunboiled samples (lanes A1 and B1).Table 1. N-Terminal sequences of the polypeptides of the puriﬁed enzyme. N-Terminal sequen ... of AF499 was identiﬁed asan archaeal promoter element by seque nce analysis. The sequ ence AAAGGTTAATATA shows a high le vel of identity with th e consensus se quence()35 to )23, AAANNNTTATATA) ... catalyticsubunit of Hdr from methanogenic archaea have beendeposited in the databases. None of these putative pro teinshas b een c haracterized and no f unction has been assigned toany of...

Báo cáo khoa học: Identiﬁcation and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot

... bp wasproduced by PCR ampliﬁcation with TOPO 2.1-SmNR1 as a template (forward primer: 5¢-ATTTCAGAAGTTGAACAAACACAC-3¢, reverse primer: 5¢-AAGATGGTATTGAAGATGATGGTTGA-3¢), puriﬁed from agarose ... DR2:5¢-CCGTAAGGTCACAAGGTCACTCG-3¢,DR3:5¢-CCGTAAGGTCACAGAGGTCACTCG-3¢, DR4: 5¢-CCGTAAGGTCACAGGAGGTCACTCG-3¢, DR5: 5¢-CCGTAAGGTCACCAGGAGGTCACTCG-3¢. PAL0: 5¢-CGCAAGGTCATGACCTCG-3¢. One strand of each oligonucleotide ... Extraction kit (Qiagen, Valencia, CA, USA) and randomly labeled with32P using a Metaprime kit (Amer-sham Pharmacia Biotech Inc., Piscataway, NJ, USA). ForBAC DNA sequencing, the BAC clone was...

Báo cáo khoa học: Identiﬁcation and characterization of a collagen-induced platelet aggregation inhibitor, triplatin, from salivary glands of the assassin bug, Triatoma infestans ppt

... Identiﬁcation and characterization of a collagen-inducedplatelet aggregation inhibitor, triplatin, from salivaryglands of the assassin bug, Triatoma infestansAkihiro Morita1, Haruhiko Isawa2, ... Western blot analysis of triplatin-1 and -2 from the salivarygland of T. infestans. Extracts from a pair of salivary glands, recom-binant triplatin-1 and recombinant triplatin-2 were separated by15% ... clonesSalivary glands of T. infestans were dissected from thoraces of unfed adults, and poly A( +) RNA was isolated from 30pairs of salivary glands using a MicroPrep mRNA isolationkit (Amersham Pharmacia...

Báo cáo khoa học: Identiﬁcation and characterization of novel salivary thrombin inhibitors from the ixodidae tick, Haemaphysalis longicornis doc

... T A P T A K P R L R G 241-AATAAGCCTTGAATCAATGATGTTCTATTTTTTATAGCGTCCCGATGGCGGTGATGTTGT -N K P * 301-AGGCTGGAAGCAAATAAAAATACGAAGAGTGACTTCAAAAAAAAAAAAAAAAAAAAAAAA A 1-GCTTTGACGGCAATGAAGCACTTCGTAATTTTGATTCTTGCTGTTGTGGCCAGTGCCGTG ... R241-CAAAATCAGGATTGAATCAATGGTGTTCTAGATTTCTATAACCTACCGACGGCGGCAATT Q N Q D *301-TTGTGGGGTCCAAACAAATAAAACTACAAAGTGGGACCTCAAAAAAAAAAAAAAAAAAAABMadanin-1 MKHFAILILAVVASAVVMAYPERDSAKEGNQEQERALHVKVQKRTDG-DADYDEYEEDGTMadanin-2 ... E Q E R A 121-CTGCATGTAAAGGTACAAAAACGTACTGATGGTGATGCTGACTACGATGAATATGAGGAA -L H V K V Q K R T D G D A D Y D E Y E E 181-GATGGGACGACTCCTACTCCGGATCCAACTGCACCAACTGCTAAACCACGGCTTCGAGGA -D G T...

Báo cáo khoa học: Puriﬁcation and properties of a new S-adenosyl-Lmethionine:ﬂavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot

... compo-nents of each reaction mixture, after their chromatographicseparation and puriﬁcation, were submitted to1HNMR(nuclear magnetic resonance) and FABMS (fast atombombardment mass spectrum) analyses ... Theconcentration of both the assayed compound and itsrespective transformation product was determined bycomparison of peak data with those obtained from authentic standards chromatographed at different ... S-Adenosyl-L-methionine:ﬂavonoid 4¢-O-methyltransferase crude activity in healthy and inoculated tissues of the carnation cultivar ¢Novada¢.In each row, values followed by a same number of * are not statistically...

Xem thêm