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Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx

Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx

Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx

... The Authors Journal compilation ª 2011 FEBS 2043 Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding ... free cysteines. In conclusion, we show a mechanism of action of bacitracin on PDI that involves covalent binding of anopen thiol form of bacitracin to free cysteines in the substrate-binding domain ... [16] tested the effect of bacitra-cin on the reductive activity of a truncated PDI con-taining the catalytic a -domain, but lacking the independent substrate-binding -domain. Bacitracin did not...
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Báo cáo khoa học: Physical properties and surface activity of surfactant-like membranes containing the cationic and hydrophobic peptide KL4 potx

Báo cáo khoa học: Physical properties and surface activity of surfactant-like membranes containing the cationic and hydrophobic peptide KL4 potx

... sys-tems showed the end of the melting process at 41 °C. The decrease of the Tm in PA-containing vesicles isexplained by the partial extraction of PA from the bilay-er by the formation of PA ⁄ Ca2+complexes. ... adsorb onto the platinum wire. An importantpoint in this step is to avoid dehydration of the sample tomaintain the integrity of the membranes. Once the materialwas adsorbed to the platinum wire, ... FEBSspecifically examine the interaction of KL4 with POPGand PA as well as the effect of these lipids on the physical properties of the membrane.Effect of KL4on the lipid order of surfactant-likemembranesTo...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt

Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt

... of b-turn in CD2 protein. In addition, the binding sites of the peptides to CD58 protein predicted by autodock were near the CD2–CD58 interface, either in the topcavityofCD58orneartheCC¢ sheet ... morepotential since it may directly interrupt the binding mech-anism of CD2–CD58. Inhibition of cell adhesion involvesinterrupting the interaction of key residues at t he protein protein interface. ... structure. In the hexapeptides, cyclization by disulfide bond w as not d esigned since a ddition of penicillamine and cysteine to form a disulfide bond willincrease the number of amino acids in the peptide....
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Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... and the recombinant protein. All sera reacting with the natural protein in the ELISA were positive in the immunoblottingexperiments. For the recombinant protein, 24 of 28 serawere negative in both ... release of the basophils, the allergen-induced histamine release was calculated as percent of the total amount of histamine determined after lysis of the basophils by twofold freezing and thawing of ... four of the investigated serareacted with the recombinant protein in the ELISA, but not in the immunoblot (not shown).IgE reactivity of the native allergen is completelyinhibited by the bromelain...
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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... density of the headgroup of the latter, leading to different migration in the HPLC purification process. Additionally, the absence of alipoprotein is confirmed by the absence of TLR2 reactivity in the ... preincubated with unlabeled LPS andrecombinant human lipopolysaccharide-binding protein LBP was added.Endotoxin activity determination by the chromogenicLimulus testEndotoxin activity of the ... signaling in the case of lipopeptides andlipoproteins [37].Although the TNF-inducing capacity of MfGl-II is lowerthan that of LPS, it is still higher with respect to the cytokine-inducing capacity...
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Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf

Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf

... in the substrate-binding domain andstrand E2 in the hinge domain, two residues appear toact, at least partly, as structural determinants for the closed conformation of the substrate loop. The ... positioning of the aromatic ring of Tyr456 in the closed state. In the absence of a closed complex of E542K P2Ox, the effect of the loss of this hydrogen bond on the packing of the substrate loop ... 2IGO, the largest difference observed is the position of the ‘head’ domain (Fig. 8). In the thermostable L537W ⁄ E542Kdouble mutant, differences in the backbone position of the exposed head domain...
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Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf

Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulfide isomerase from Conus marmoreus pdf

... the x-intercept of the RNase activity plot. The oxidase activity matches the slope of the linear plot of reciprocal of lag timesagainst the PDI concentrations in units of lm)1Æmin)1. The Z ... For the rate of decrease of the linear form, the original data were fitted by Y(t) ¼ e–kt· 100%, where Y is the percentage of the linear form, and t is the refolding time. For the rate of increase ... dithiols to form disulfide bonds [16], and both of them are present in the ER lumen. To compare the roles of these differentoxidants during the folding of conotoxins, the refolding of reduced tx3a...
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Báo cáo khoa học: MicroRNA-373, a new regulator of protein phosphatase 6, functions as an oncogene in hepatocellular carcinoma pdf

Báo cáo khoa học: MicroRNA-373, a new regulator of protein phosphatase 6, functions as an oncogene in hepatocellular carcinoma pdf

... compared with the control groups. In addition, we constructed another EGFP reporter vec-tor containing mutations in the regions encoding the miR-373 binding sites (Fig. 4A). Neither blocking of miR-373 ... of miR-373 with ASOs nor overexpression of miR-373had any effect on the intensity of EGFP fluorescencefrom the vector containing mutations in the regionencoding the miR-373 binding sites. These ... encoding the putativebinding site, was inserted downstream of the EGFPcoding region. HepG2 cells were transfected with the reporter vector together with either miR-373 ASOs orpri-373. As shown in...
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Báo cáo khoa học: Ca2+ ⁄ H+ antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes pdf

Báo cáo khoa học: Ca2+ ⁄ H+ antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes pdf

... uptake into liposomes as a result of the decrease in pH wasassayed by measuring the radioactivity of tritium, as described in Materials and methods (In the figure, the y-axis represents the cpm ... When the proteoliposomes weresuspended in the indicated acidic buffer solutionscontaining [3H], the radioactivity inside the proteolipo-somes increased with decreasing external pH, indica-tive ... performed with liposomes in the absence of reconstituted BI-1. Acidic or alkaline conditions didnot induce the release of encapsulated Ca2+ in the absence of BI-1, when assayed using indo-1 and45Ca2+....
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Báo cáo khóa học: Purification and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus ppt

Báo cáo khóa học: Purification and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus ppt

... insecticidal activity of these proteins hasbeen evaluated, it is unclear whether the toxins in the regurgitant of antlions are limited to these two proteins,and whether other symbionts of the larvae ... obtained by tryptic digestion of the protein. Cloning and sequencing of the insecticidal toxin (SMC)gene of B. cereus The SMC-encoding gene was amplified by PCR using 1 UKOD Plus polymerase, the ... when the proteins were stained with Coomassie Brilliant Blue R250 (Nacalai tesque Inc.,Kyoto, Japan).Sequencing of the proteinous toxin produced by B. cereus The N-terminal and internal amino-acid...
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