Báo cáo khoa học: N-glycosylation inﬂuences the structure and self-association abilities of recombinant nucleolin pot

Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

... consti-tute the protein structure. Here, we report the analysis of the contribution of these putative domains to the structure/ function of recombinant human GCPII. We cloned 13mutants of human ... absence of the intracellular and transmem-brane domains does not inﬂuence carboxypeptidase activity of GCPII and neither does the attachment of the His-Xpress epitope at the N-terminus of the 44/750 ... NAAG-hydrolyzing activity (the 44/736 variant), and the C-terminal extension (addition of the V5-His tag inthecaseofthe44/750_V5-Hisvariant)reducedtheactivitybyafactorof>104. Furthermore, C-terminal...

Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt

... works 4453 of the S1-bound chloride anion and ‘pushes’ the Cl)out of the bottom of the S1 pocket (the occupancy of the Cl)in this structure is 0.7). The alternate confor-mation of the Asp443 ... with the occupancy of the S1 pocket by a ligand and the ‘open–close’ transition exploits the hinges on the twosides of the lid made up of Asn540 ⁄ Trp541 and Gly548 (GCPII numbering). In the ... disallowed Ramachandran conformation.Such repositioning was not observed in any of the reported structures of GCPII, although the analysis of the thermal parameters of the recombinant humanGCPII...

Báo cáo khoa học: Sulfated polysaccharides inhibit the catabolism and loss of both large and small proteoglycans in explant cultures of tendon doc

... inclusion of these sulfated agents there was signiﬁcant inhibition(40%) of rate of loss of 35S-labeled large proteogly-cans from the matrix by the end of the culture period.Similarly, by day 10 of ... that the rate of [35S]sulfate appear-ing in the medium was suppressed by the inclusion of the sulfated polysaccharides. At the end of the cultureperiod, both CaPPS and heparin reduced the ... appeared in the mediumon each day of culture and that remaining in the matrix at the end of the culture period [41]. From these data, the per-centage of 35S-labeled proteoglycans remaining in the...

Báo cáo khoa học: "Conundrums in Noun Phrase Coreference Resolution: Making Sense of the State-of-the-Art" docx

... B3presumesthat the gold standard and the system response areclusterings over the same set of CEs. This, of course, is not the case when the system automat-ically identiﬁes the CEs, so the scoring ... coreference elements, and knowledge of all and only the annotated CEs. We also measure the performance of state -of -the- art resolvers on sev-eral classes of anaphora and use these results todevelop ... weighted sum of the observed scores for eachresolution class (i.e. the mean for the class mea-sured on the other ﬁve data sets) weighted by the proportion of CEs that belong to the class. The predicted...

Tài liệu Báo cáo khoa học: A facile method for expression and puriﬁcation of the Alzheimer’s disease-associated amyloid b-peptide pdf

... understanding of the structure of both the Ab monomer and its various assemblies could help in the design of new therapeutic strategies targeted atpreventing the formation or ameliorating the activity of ... Subramanian and Shree[42], there was no rigorous assessment of the purity of the product or the correctness of the sequence. In con-trast, the purification protocol that we have developedis quick and ... significantlyincreases the cost of the purification, and the require-ment for HPLC increases the length and complexity of the purification procedure. Moreover, as with the studies by Lee et al. [21] and Subramanian...

Báo cáo khoa học: New insights into the functions and N-glycan structures of factor X activator from Russell’s viper venom pot

... plate-lets and endothelial cells. The important role of theseglycans in pharmacokinetics has been demonstrated by the slower and smaller increment of FDP in vivo after the injection of desialylated ... sequenced. Of these, 10clones were found to encode the LC2 and LC1 sub-units. Others were found to encode other variants of the C-lectin-like venom proteins. The amino acidsequences of both subunits ... signalpeptide of 23 residues, and the predicted sequence forits mature protein matches that published previously[8]. The amino acid sequences of LC1 and LC2, togetherwith those of other homologues of...

Báo cáo khoa học: Puriﬁcation, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt

... purifiedfrom the gut of larvae of the yellow-spotted longicorn beetlePsacothea hilaris by acetone precipitation and elution fromgels after native PAGE and SDS/PAGE with activitystaining. The purified ... cockroaches and crayfish) and GHF 45 (mussel and beetle). These threefamilies are structurally unrelated and their evolutionaryorigins are likely to be independent.Larvae of the yellow-spotted longicorn ... constitute only 4.7% of the dry weight of the wood [8]. The habitat of P. hilaris larvae suggests that theypossess the ability to digest cellulosic materials. In fact, avariety of carbohydrase activities,...

Báo cáo khoa học: Metal ions modulate the folding and stability of the tumor suppressor protein S100A2 docx

... on the thermodynamic stability of the Ca2+- and Zn2+-loaded mutants can be retrieved from the anal-ysis of metallation effects within the background of the same mutation (Table 2). With the ... to the high-afﬁnitysite.These effects of metal ions on S100A2 folding and stability contribute to a better understanding of the Ca2+- and Zn2+-dependent regulation of the protein.In the ... mechanism of Zn2+- and Ca2+-dependent activation of S100 proteins. The anta-gonistic effect of Zn2+ and Ca2+in the control of S100A2 stability provides a molecular rationale for the action of...

Báo cáo khoa học: Atg8L/Apg8L is the fourth mammalian modiﬁer of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3 docx

... taggedprotein. Merging of the images indicated that most of the puncta of CFP–Atg8L were colocalized with those of YFP–LC3 (Fig. 4,f,I,l). In the absence of inhibitors,only a few puncta of either type were ... [21]. The aminoacid sequence of mouse Atg8L ⁄ Apg8L shows 100% and 54% identity to those of human Atg8L and yeastAtg8, respectively, and 33%, 60% and 86% identity to the amino acid sequences of ... cleavage of the C-terminus of Atg8L. Wild-typeTRX–Atg8L)3xFLAG was expressed in Escherichia coli; the cellswere lysed and centrifuged, and the supernatants were used as the substrate. The supernatants...

Báo cáo khoa học: Structural basis for the changed substrate speciﬁcity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx

... comparing the phosphorylation of the natural substrates of the double mutant with the singlemutant, it seems that the mutation N45D in the double mutant counteracts the negative effect(s) of the N64D ... 3737actions with the 3¢-OH of the deoxyribose of the substrate. The absence of this hydrogen bond and aﬂexible LID make the substrate binding pocket larger and provide space for the bulky 3¢-azide ... than in the wildtype enzyme. The IC50value for dT phos-phorylation is increased more than 100-fold. The structure of the Dm-dNK N64D mutant pre-sented above and the understanding of the feedbackregulation...

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