Báo cáo khoa học: Mitochondrial transcription factor A overexpression and base excision repair deﬁciency in the inner ear of rats with D-galactose-induced aging pdf

... accelerated aging of d-Gal-treated animals [21,22]. These charac-teristics resemble those of the natural aging process in humans and other animals. As the inner ear tissue isunacquirable during ... study in mice also reported that the capacity to repair oxidative DNA damage in various brain regionsduring aging was altered in an age-dependent manner, and increased mtDNA oxidative damage might ... deletion in the inner ear of rats with d-Gal-induced aging. Mitochondrial DNA deletions can accumulate with aging in postmitotic tissues with high energeticdemands, such as skeletal muscle, heart,...

Báo cáo khoa học: Tumor necrosis factor-a converting enzyme is processed by proprotein-convertases to its mature form which is degraded upon phorbol ester stimulation pptx

... disintegrin and metalloproteinase) family of type I membrane proteins and mediates the ectodomain shedding of various membrane-anchored signaling and adhesion proteins. TACE is syn-thesized as ... anti-TACE, and a polyclonal rabbit antibody againstendogenous TACE (Chemikon International, Temecula,CA). Both antibodies are directed against the C-terminalpart of the proteins and therefore ... decreases the amount of mature TACE (Fig. 5).Discussion The prodomain of the catalytically active members of the ADAM family is thought to act as an inhibitor of the proteinase via a cysteine...

Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx

... in obesity-associated insulin resistance and diabetes, inhibit insulin signalling atseveral points in the signalling cascade. The liver is critical in maintaining cir-culating glucose levels and, in a ... onphosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cellsAmit K. Pandey, Vikash Bhardwaj* and Malabika DattaInstitute of Genomics and ... SanLeandro, CA, USA). The same sized rectangular box wasdrawn surrounding each band and the intensity of each wasanalysed by the program after subtraction of the back-ground intensity.Statistical...

Báo cáo khoa học: Tumor necrosis factor-a-induced caspase-1 gene expression pptx

... processes, including celldeath, cell growth, stress response and in ammation[2,3]. One of the major signaling pathways induced byTNF -a leads to the activation of transcription factor nuclear factor- jB ... stimulation of the human lung carcinoma cell line, A5 49, with TNF -a increases the expression of caspase-1 mRNA and pro-tein. The increase in caspase-1 gene expression is prob-ably caused by activation ... [19].However, the transcription factors involved in the acti-vation of the caspase-1 promoter by TNF -a are notknown. In the present study we analyzed the role of p73 and IRF-1 in mediating TNF -a- induced...

Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc

... for the treatment of ﬁbrosis, cancers, and cardiovascular and metabolicdiseases.Materials and methodsMaterialsAn antibody against Sp1 was purchased from Active Motif(Carlsbad, CA, USA). Antibodies ... for ampliﬁcation of the NOX4 promoter were asfollows: 5¢-AACAATCAGTCTAAAAGAGCTGTGTCTTCT-3¢ (forward for GC-box1), 5¢-CTCCAAAATACTGGCAAACATGTGAACAAT-3¢ (reverse for GC-box1), 5¢-TGAGTGGGCAGAGCTGACCCGGTGCGGGT-3¢ ... by the upper band, was inhibited by the antibody. On the other hand, pre-incubation with an anti-Sp3 IgG gen-erated supershifted bands (as indicated by arrowheads) and decreased the intensity of...

Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

... calpast-atin into the cytosol without affecting Ca2+homeostasis. The fact that the changes in the calpain state are also observed under the latter conditionsindicates that calpastatin availability ... observations indicating that calpain and calpast-atin can associate in a 1 : 1 molar ratio, regardless of the presence of Ca2+(unpublished work). In addition to Ca2+ and calpastatin, a number of digestible ... 2006)doi:10.1111/j.1742-4658.2006.05180.xConformational changes in the calpain molecule following interaction with natural ligands can be monitored by the binding of a speciﬁc monoclonalantibody directed against the catalytic domain of the...

Báo cáo khoa học: Abortive translation caused by peptidyl-tRNA drop-off at NGG codons in the early coding region of mRNA potx

... 1 Plasmid constructs are derivatives of pDA3480 [9,10]. The 5¢ end of the transcript is 5¢-AAUUGUGAGCGGAUAACAAUUUCA-CCAGGUAAUAAAUUAAAUAAAAUUUAAAUAUG-3¢ for the gene variants that lack a functional ... a plasmid with +5AGG in the test gene was combined in the MB01strain with the plasmid pUBS520 that carries the tRNAArg4gene, or plasmid pArgUW with the tRNAArg4(decodes AGA and AGG) and ... wild-type strain was com-pared using a plasmid encoding tRNAArg4(decodingAGA ⁄ AGG) and tRNAArg5(decoding AGG) and another plasmid with the 3A test gene, using doubleantibiotic selection. As shown...

Tài liệu Báo cáo khoa học: Mitochondrial chaperone tumour necrosis factor receptor-associated protein 1 protects cardiomyocytes from hypoxic injury by regulating mitochondrial permeability transition pore opening docx

... The targeting sequence of the siRNAagainst rat TRAP1 was 5¢-CAACAGAGATTGATCAAAT-3¢. A negative control adenovirus vector containingnonspeciﬁc siRNA was constructed in the same way (non-speciﬁc ... expression increases in cardiomyocytes, the role of that TRAP1 increaseremains unclear. The question remains as to whether the hypoxia-induced TRAP1 increase is a protectivereaction in cardiomyocytes. ... protein 1 (TRAP1) is a mito-chondrial chaperone that plays a role in maintaining mitochondrial func-tion and regulating cell apoptosis. The opening of the mitochondrial permeability transition...

Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

... mitochondrial targeting of CYP2E1Naresh B. V. Sepuri, Sanjay Yadav, Hindupur K. Anandatheerthavarada and Narayan G. AvadhaniDepartment of Animal Biology, School of Veterinary Medicine, University of ... erythromycin, waspreincubated for 3 min. The reaction was initiated by the addition of 3 mm NADPH, and continued for 30 min at37 °C in a shaking water bath. The reaction was terminatedby the addition ... that in yeast, PKA controls the nuclearlocalization of certain transcription factors, such asMsn2 and Msn4, and of snf1 kinase in the cytosol in N. B. V. Sepuri et al. Mitochondrial targeting of...

Báo cáo khoa học: Epidermal growth factor receptor-regulated miR-125a-5p – a metastatic inhibitor of lung cancer potx

... against Aktwas from BioVision, Inc. (CA, USA), and antibody againstphospho-Akt was from Santa Cruz Biotechnology, Inc.(Santa Cruz, CA, USA).Western blot analysisTo examine the in uence of ... miR-12 5a- 5p expression in a subset of human lung cancersTo gain further insights into the role of miR-12 5a- 5p in lung carcinogenesis and to examine the clinical rele-vance of our ﬁndings, we investigated ... implicated in the formation of neoplasms, malignant progression, and metastasis.To examine the potential miRNA targets of EGFR sig-naling, we used the miRHuman_10.0_070802 miRNAarray, and identiﬁed...

Xem thêm