Báo cáo Y học: Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y pot
Báo cáo Y học: Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y pot
... doi:10.1046/j.1432-1033.2002.02997.xdesigned to maintain hydrophobicity and Gly, Ser, Asp andHis mutants to provide hydrophilicity. The catalytic roles of Cys341 in carboxypeptidase Y are examined by comparing the kinetic parameters of ... Amphipathic property of free thiol group contributes to an increase in the catalytic efficiency of carboxypeptidase Y Joji Mima, Giman Jung, Takuo Onizuka, Hiroshi Ueno and Rikimaru HayashiDivision ... import-ant for substrate binding, although it may not necessarilyhave any effect on the rate of acylation.Roles of Cys341 in the catalytic mechanism of carboxypeptidase Y Before the formation of the...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
... peripheral nervous system injury, and reducing the biosynthesis of ICAM-1 and other cytokines by blocking the cell signal pathway mightprovide a new strategy against in ammatory and immune reaction ... p38 and JNKleads to an increase in ICAM-1 expression in Schwanncells of sciatic nervesAiguo Shen1,*, Junling Yang2,*, Yangyang Gu3, Dan Zhou4, Linlin Sun2, Yongwei Qin2,Jianping ... in ammatorysites [35,36]. SCs have been implicated in human in ammatory demyelinating neuropathies such asGuillain–Barre´syndrome and chronic in ammatorydemyelinating polyneuropathy [31]. In...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... domain,converting some of the enzyme to the active form. The 3-fold increase in activity may arise from the binding of the FMN domain to hMS facilitating binding of AdoMet and ⁄ or methyl transfer, ... cobalamin affinity chromatography, as judged bySDS ⁄ PAGE analysis (Fig. 2, inset). The absorptionspectrum of the purified enzyme was typical of the hydroxycobalamin form of the enzyme (Fig. 2). The recovery ... usingtwo steps, employing ion exchange chromatographyfollowed by cobalamin affinity chromatography(Table 1). The affinity chromatography step conve-niently converts the apo-form of hMS into the...
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Báo cáo khoa học: MicroRNA-143 reduces viability and increases sensitivity to 5-fluorouracil in HCT116 human colorectal cancer cells potx
... Consequently, strategies aiming to over-come tumour cell resistance to chemotherapy and to increase drug efficacy, thereby minimizing toxic effects,are critically important. The molecular mechanisms of 5-FU ... been used in the clinic forseveral decades, and its metabolic pathways and cyto-toxic modes of action through the inhibition of thymi-dylate synthase activity and incorporation into RNAand DNA ... of 5-FU cytotoxicity have been characterized, especiallyits ability to incorporate into RNA and DNA and to inhibit thymidylate synthase. 5-FU has recently beenshown to modulate miRNA expression in...
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Báo cáo khoa học: "PARALLEL MULTIPLE CONTEXT-FREE GRAMMARS, FINITE-STATE TRANSLATION SYSTEMS, AND POLYNOMIAL-TIME RECOGNIZABLE SUBCLASSES OF LEXICAL-FUNCTIONAL GRAMMARS" docx
... as an input, starts from the initial state with its head scanning the root node of an input. Ac- cording to the current state and the label of the scanned node, it transforms an input tree into ... locally satisfies the functional schemata of all productions in sp. M transforms r into the yield of t, i.e., the terminal string ob- talned by concatenating the labels of leaves, in a top-down ... Department of Information and Computer Sciences, Faculty of Engineering Science, Osaka University 1-1 Machikaneyama, Toyonaka, Osaka 560, Japan :~ Graduate School of Information Science, Advanced Institute...
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Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
... of the solubilization of SugE, Y K and TehA in micelles and of their reconstitution intomembranes. The dissolving of the proteins in suitabledetergents obviously resulted in the refolding of ... part of the synthesized protein‘remained soluble and this amount could be significantlyincreased by the addition of E. coli lipids into the cell -free reaction. Alternatively, the majority of the ... assembled human T-cellreceptor by in vitro expression [37]. The cell -free expressionprincipally offers the opportunity to insert the translatedprotein directly into the desired membrane of choice.Tedious...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
... for the catalytic action of plas-min in the presence of stearate. The p-nitroaniline (P) released in the course of hydrolysis of 40 lM Spectrozyme-PL by 20 nM plas-min in the presence of 115 ... manner, the instability of the enzyme in the assay system was also consid-ered according to the scheme suggested by Duggleby [13,14] in which E ¢ indicates the inactive form of the enzyme, andJ1, ... activity of two truncated plasmin variants was examined(Fig. 8). Miniplasmin (des-kringle 1-4 plasmin) con-tains the kringle 5 and the catalytic domain of plas-min, whereas microplasmin (des-kringle...
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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx
... between the volume change introduced by the mutation in position266 and the decrease in enzyme catalytic efficiency. Remarkably, the value for the catalytic efficiency pre-viously obtained by replacement ... primarilyinvolved in nicotinamide binding rather than beingdirectly involved in the hydride transfer reaction [21].Our results, together with the amino acid arrangementobserved in the catalytic ... to analyze the geometry of the tyrosinestacked on the si-face of the flavin in FNRs [12]. The single point energies of the flavin–tyrosine system in different conformations were calculated. The...
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Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... quantification of the local background and evaluation of the array homoge-neity, which is taken into account in the normalization.Furthermore, in order to consider the purity and quality of the ... using a factor calculated from the intensity ratios of internal standards in the test and refer-ence samples. The presence of the internal standard probesat different locations of the array ... small interferingRNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNAoff-target effectsSe´bastien Vankoningsloo1, Franc¸oise...
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Báo cáo khoa học: Wheat germ cell-free platform for eukaryotic protein production potx
... aremuch lower than for prokaryotic targets. In the inter-est of efficiency and cost savings, it is important to analyze where failures occur and to devise strategies to minimize these. The most effective ... exhibiting £ 30% sequence identity to the sequence of any protein with a three-dimensionalstructure deposited in the Protein Data Bank. Solubil-ity can be improved greatly by producing the protein of ... as not to dis-turb the labeling pattern.Cell -free systems have been used for the production of various kinds of proteins, including membrane pro-teins [14] and proteins that are toxic to cells...
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Từ khóa: and an increase in the presentation of the endogenous lectin galectin1 sensing these changes on the surface of p16 ink4a expressing pancreatic carcinoma cells capan1upon inception of the obligation giving rise to the indemnity or payment or for subsequent changes in these amounts that result in an increase in the provision with a debit to the corresponding accounts in group 6upon inception of the obligation or for subsequent changes in these amounts which result in an increase in the provision generally with a debit to accounts in subgroup 21effect of an increase in the u s dollar value on real gnpeffect of an increase in the quality of audit prescribed by auditing standards onbáo cáo y họcbáo cáo khoa học y họcbáo cáo y họcbáo cáo y học cổ truyềnbáo cáo y tế học đườngmẫu báo cáo y tế học đườngbáo cáo y tế học đường cuối nămbáo cáo y tế học đường năm 2012báo cáo y tế học đường trường mầm nonbiểu mẫu báo cáo y tế trường họcchuyên đề điện xoay chiều theo dạngNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXBT Tieng anh 6 UNIT 2Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ