Báo cáo Y học: Protein methylation as a marker of aspartate damage in glucose-6-phosphate dehydrogenase-deﬁcient erythrocytes docx

... Protein methylation as a marker of aspartate damage in glucose-6-phosphate dehydrogenase-deﬁcient erythrocytes Role of oxidative stressDiego Ingrosso1,2, Amelia Cimmino1, Stefania D’Angelo1, ... thecirculation [6,25,26]. This has been related to an increasednumber of abnormal aspartate residues, spontaneouslyarising fromL-asparaginyl deamidation and/orL-aspartylisomerization reactions ... S-adenosylmethionine(AdoMet)-dependent enzyme,L-isoaspartate (D -aspartate) protein O-methyltransferase (PCMT; EC 2.1.1.77) [11,12].Enzymatic methylation of abnormal aspartate residues isphysiologically...

Báo cáo khoa học: 2-Pyrimidinone as a probe for studying the EcoRII DNA methyltransferase–substrate interaction docx

... containing DNA as a MTase inhibitor.2-pyrmidinone incorporation in DNA sequences may alsoserve as a speciﬁc probe for studying discriminationcontacts formed by proteins and functional groups of pyrimidine ... proximity of the cofactor [2].Several cytosine analogues, 5-ﬂuorocytosine (FC),5-azacytosine (AzaC) and 2-pyrimidinone (2P), have beenreported as mechanism-based inhibitors of C5 MTases[3–6]. Introduction ... study the mechanism of inhibition of M.EcoRII by DNAcontaining 2-pyrimidinone, a cytosine analogue lacking anNH2group at the C4 position of the pyrimidine ring. Also,DNA containing 2-pyrimidinone...

Báo cáo khoa học: Missense mutations as a cause of metachromatic leukodystrophy Degradation of arylsulfatase A in the endoplasmic reticulum potx

... immunopre-cipitation of Tyr201Cys substituted ASA with mAb C.Degradation of amino acid-substituted ASAsvia the proteasome In order to investigate the degradation pathway of amino acid-substituted ASAs in ... caused by the deficiency of arylsulfatase A (ASA). This enzyme catalyses the firststep in the degradation pathway of the glycosphingo-lipid 3-O-sulfogalactosylceramide. Deficiency of theASA causes ... homogen-ates with the polyclonal ASA antiserum.Precipitated ASA was quantified afterSDS ⁄ PAGE with a bio-imaging analyser(Fujifilm). Columns show mean, minimal andmaximal deviation of arbitrary...

Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... subcellular U937fractions. Cystatin F concentrations were measured with a specificELISA, total protein concentration was measured with Coomassiebinding assay and b-hexosaminidase activity was measured ... cystatin F and ER the stainingwas performed on cells cultured for 2 days. Cystatin F wasstained as described above and after the last washing step20 lgÆmL)1Texas Red conjugated concanavalin A (Molecular ... amino acids long andcontain at least two disulfide bridges. Type 3 cystatins, thekininogens, are larger proteins containing three tandemlyrepeated type 2-like cystatin domains. Type 2 cystatinsidentified...

Tài liệu Báo cáo khoa học: Phage-display as a tool for quantifying protein stability determinants pptx

... recovereddifferentially based on varying stabilities. Remarkably, wehave found that, at least in some circumstances, a quanti-tative correlation to biophysical data can be obtained from a statistical analysis ... the association of the variable heavy chain (VH)with protein A was used as a surrogate for direct stabilitymeasurements. The VHdomains in camelid heavy chainantibodies are most similar to ... to a variety of denaturing conditions. With theB1 domain of protein G (GB1) and a camelid heavy chainantibody as model systems, we are using phage-display lib-raries to experimentally address...

Báo cáo Y học: The mitochondrial-lysosomal axis theory of aging Accumulation of damaged mitochondria as a result of imperfect autophagocytosis ppt

... withinlipofuscin-loaded lysosomes. Apparently, cells have a limitedcapacity to produce lysosomal enzymes, and as lipofuscin-containing lysosomes act as sinks for newly synthesizedenzymes, they are not available ... jeo-pardize the stability of lysosomal membranes, resulting in leak of a variety of hydrolytic enzymes into the cytosol. Such lysosomal enzymesmay directly activate Bid, pro-caspases, or lytic cytosolic ... recyclingmechanisms are inherently imperfect [15,25], and this mayprovide an attractive explanation for many of the features of aging. A number of early explanations of aging, such as Orgel’s error catastrophe...

Báo cáo Y học: Domain organization, folding and stability of bacteriophage T4 ﬁbritin, a segmented coiled-coil protein docx

... ﬁbritin XN that hadno C-terminal domain. Our goal was to answer a questionabout how many thermodynamically independent domainsﬁbritin has, and to assign the thermal transitions toindividual ... and F proteins wereadditionally applied to a 15-mL DEAE–Sephacryl columnand eluted w ith a linear gradient o f NaCl. Fractionscontaining proteins were dialysed against Tris/EDTA bufferandstoredat4°C.The ... Sequencingwas carried out by the d ideoxy chain termination methodusing a DNA sequencing kit/BigDye terminator cyclesequencing ready reaction (Applied Biosystems) a nd anautomated DNA sequencer.Expression...

Báo cáo Y học: Cytochrome c from a thermophilic bacterium has provided insights into the mechanisms of protein maturation, folding, and stability potx

... Kobayashi2, Yasuo Igarashi3and Jun Hasegawa41Graduate School of Biosphere Sciences, Hiroshima University, Japan;2Faculty of Pharmaceutical Science, Osaka University, Japan;3Department ... factors that affect protein stability, weattempted to achieve maximal enhancement of the stability of PA c-551 by introducing minimal mutations into spa-tially separate regions. Five amino-acid ... andfolding as well as stability. Bacterial cytochrome c hascontributed greatly to our understanding in these areas, andis one of the most successful model proteins. The basic ideasobtained with cytochromes...

Báo cáo y học: "Do we need a critical care ultrasound certification program? Implications from an Australian medical-legal perspective"

... perform a vaginal hysterectomy that was beyond the capacity of the registrar in question. As a result of poor surgical skills, the patient’s fallopian tube was entrapped in the suture line and later ... accepting the fee, the doctor has a contractual duty to provide a AbstractMedical practitioners have a duty to maintain a certain standard of care in providing their services. With critical care ... of care e requisite standard of care is reasonable skills and care reasonably expected of a practitioner with the same standing.  e standard of care is diﬀ erent in cases of diagnosis and...

Báo cáo y học: " High blood pressure, antihypertensive medication and lung function in a general adult population"

... function in a gen-eral adult population. Thus, our findings may serve as a basis for experimental testing, as for example by addingmeasurement of respiratory functions to outcomes of ‘hypertensive’ ... measurements were taken with a 3-minute pauseafter a rest of at least 5 minutes in a sitting position on theright arm. The mean of the last two measurements wasused for the current analyses. ... Germany.6Department of Internal Medicine B - Cardiology, Intensive Care, Pulmonary Medicine andInfectious Diseases, University of Greifswald, Greifswald, Germany.7CentralHospital of Augsburg, MONICA/KORA Myocardial...

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