... enantiomers of glutamate,asparagines, glutamine, alanine, serine, lysine, and arginine,were inactive as substrates. The enzyme was not inhibitedby these nonsubstrate amino acids (10 mM), N-methyl-DL-aspartate ... enzyme gene in pBASPR was verified in both directions in the same way asdescribed above. Owing to t he re-cloning of the enzymegene into the Nco I-HindIII site of pATE19, the secondamino acid ... AS-N(5¢-CATGCCATGGGACGACCATTTTTTGCG-3¢), in which an NcoI site (underlined) is incorporated, and theantisence primer AS-C (5¢-CCCAAGCTTCTAGCGGCGGATGGCCTTGGC-3¢), in which a HindIII site (under-lined) i s included. The...