... because of the large size of some of the introns, they are not. For stat6, potentialmissing human exons in zebrafish are indicated by ‘*’. The EMBL accession numbers of the T-bet genes are as follows: ... zffoxp3-R2 for foxp3. These primers amplified part of the initial predictedsequence which contained the majority of the open readingframes of zebrafish stat6, t-bet and foxp3 to check they werecorrect. ... internal control for RT-PCR. PCR condi-tions were as follows: one cycle of 94 °C for 3 min, 32cycles of 94 °C for 30 s, 54 °C for 30 s and 72 °C for 30 s,followed by one cycle of 72 °C for 5 min....