Báo cáo khoa học: Functionally active fusion protein of the novel composite cytokine CLC/soluble CNTF receptor pdf
Báo cáo khoa học: Functionally active fusion protein of the novel composite cytokine CLC/soluble CNTF receptor pdf
... m.Gene. Ther. 11, 1177–1187.Ó FEBS 2002 Bioactive CLC/sCNTFR fusion protein (Eur. J. Biochem. 269) 1941 Functionally active fusion protein of the novel composite cytokine CLC/soluble CNTF receptor Catherine ... Binding of the cytokine to the membrane-bound, nonsignaling a chain(CNTFR; [21]), l eads to the recruitment of t he sha redsignaling subunits gp130 a nd the LIFR with the formation of the high-affinity ... precursor protein were maintained in the fusion protein with CLC in order to allow f or the secretion of the protein in mammalian cells. A c-myc epitope tag wasintroduced at the C-terminus of the...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... respectively, of that of HPRT. This low activitycould be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... andregulators of other enzymes in the human nucleotidemetabolism (Table S1). The library can then be seen toconstitute the specific metabolome of the nucleotidemetabolism, which would be the most ... strength [20].In the present study, we report the structural andfunctional characterization of the human HPRThomolog PRTFDC1. To shed light on the potentialfunction of PRTFDC1, the protein was...
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Tài liệu Báo cáo khoa học: Post-translational modification of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx
... stabilization of active RhoA by OTUB1 prior to bacterial invasion. The accumulated pool of active RhoA contributes toan enhanced uptake in the early phase of invasion, con-sistent with the involvement of ... KesslerDepartment of Clinical Medicine, University of Oxford, UKIntroduction The genus Yersinia consists of three pathogenic speciesthat are agents of a variety of diseases, one of whichwas historically the ... essential forYersinia pathogenesis [19]. The Rho family of small G proteins represents alarge group of the Ras superfamily of GTPases. Morethan 20 proteins of this class have been described todate,...
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Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... centrifugation of the samples, proteins bound to liposomes were analyzed bySDS ⁄ PAGE of the resulting pellets. (C) The amounts of ICin the pellets. The amounts of ICwere quantitated with the UN-SCAN-IT ... phospholipid and membrane binding of the other members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, includingthose of the binding of ICto phospholipid membranes,are ... the fluorescence of IC–GFP was observed at the FM4-64-stained vacuolar membranes and also the vacuolarlumens in the majority of yeast cells (70% of the cellsgrown at 72 h; right panels of Fig. 3A,B). Therefore,the...
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Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf
... that the fusion peptides of gp41 of HIV-1 and HA2 do not span both leaflets of the membrane bilayer [ 15,22]. The I FP of ASLV-A is shown topenetrate more shallowly into the membrane than the N-terminal ... in analogy to the IFP of class II fusion proteins [10,11]. In conjunction with the model proposed for the class II fusion proteins, it is c oncluded t hat all the v iralFPs and fusion loops examined ... class II fusion protein which uses a Ôcd loopÕto insert into the target membrane in the fusion process[10,11]. In the following, a variety of physical properties of the putative IFP of ASLV...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
... proliferation of LXF-289 lung tumor cells.Effects of P2 receptor agonists. The influence of the different P2 receptor agonists is shown as percentage inhibition of the prolifer-ation of LXF-289 ... per-centages of the cells in the G0 ⁄ G1, S and G2 ⁄ M phases of the cellcycle after treatment with 20 lM or 100 lM ATP for 24 h. The gat-ing used for the quantification of the cells in the G0 ⁄ ... tounderstand how the different P2 receptor subtypescontribute to the regulation of cancer cell prolifer-ation.In the majority of cell lines, P2Y receptors mediate the inhibition of growth or proliferation,...
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... and by increasing the pKa of the catalytic acid. The hydroxyl group of Tyr214 makes a major contribution to the positioning of the N-acetyl group of the )1sugar.Takentogether, the results show ... 257rows 5 and 7 shows that in the presence of the substrate,rotation of Asp142 towards Glu144 lowers the pKa of the latter by 0.8 pH units. The calculated effects of the D140N and D215N muta-tions ... for the position of residue 142 [34]. The pKacalculations indicate that the only candidate fora residue determining the basic arm of the pH-activityprofile of the D142N mutant is Glu144. The...
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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
... these residues stabilize the distortion of the NAG3 residue. In these interac-tions, we particularly focused on the Trp664 residue,which is located at the bottom of the active site cleft. The ... result of con-formational changes, but of the removal of negativecharge at these residues.Conformation of chito-oligosaccharides bound to the active site cleftFor the structural determination of ... Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B). The structures of boundsugars and the side-chains of three acidic residues in the conserved...
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Báo cáo khoa học: CHIP participates in protein triage decisions by preferentially ubiquitinating Hsp70-bound substrates pdf
... analyzed the influence of CHIP on the ATPasecycles of both chaperones in the presence of co-chaperones and a substrate,and determined the ubiquitination efficacy of CHIP in the presence of the chaperones. ... influences the chaperone cycle of Hsp70 proteinsto prolong the life-time of the substrate–Hsp70–CHIPcomplex and thereby to increase the possibility of recruitment of the E2 ubiquitin-conjugating ... did notreduce the inhibitory effect of p23 on the ATPaseactivity of Hsp90, we determined whether this is dueto the inability of CHIP to bind to Hsp90 in the pres-ence of p23. We therefore incubated...
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Báo cáo khoa học: Identification and expression of the first nonmammalian amyloid-b precursor-like protein APLP2 in the amphibian Xenopus laevis ppt
... in the brain and further displays anumber of structural features not present in the othermembers of the APP superfamily, such as the absence of the exon encoding the KPI domain and the lack of ... [3,4,13,26].Comparative analysis of the Xenopusand mammalianAPLP2 proteinsComparing the amino acid sequences of the twoX-APLP2 proteins with the human, mouse and ratAPLP2 protein sequences showed ... reared at the Central Animal Facility of the University of Nijmegen. Experimental procedures were performedunder the guidelines of the Dutch law concerning animalwelfare.Rapid amplification of 5¢...
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