Báo cáo khoa học: The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides pot

... al. (Eur. J. Biochem. 269) Ó FEBS 2002 The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides Anneloor L. M. A. ten ... as a single band on t hese gels, as the ODN h ybridizes to the center of the input target RNA. The asterisk indicates the input targetRNA; the arrow indicates the digested product bands. The amount ... 7. Staining pattern of cells expressinggreen-¯uorescent protein (GFP) and GFPfused to RNase H1 (GFP H1) or R Nase H2 (GFP H2) .Ó FEBS 2002 RNases H and variation in cellular response to ODN...

Báo cáo khoa học: a-1 Antitrypsin binds preprohepcidin intracellularly and prohepcidin in the serum pdf

... (http://www.ncbi.nih.gov/blast/Blast.cgi).Binding of A1AT to preprohepcidin, prohepcidin and mature hepcidin The cDNA of A1AT was cloned into pTRG with the restric-tion sites EcoRI and XhoI, and the coding cDNAs of prep-rohepcidin, ... secretion. To generate the mature25-AA hepcidin peptide, there is a furin cleavage site in the C-terminal part of prohepcidin.E. Pandur et al. In vivo interactions of preprohepcidin and prohepcidinFEBS ... MALDI-TOF) and in vivo (ELISA, cross-linking assay). Our results demonstrated that the serine protease inhibitor a-1 antitrypsin binds preprohepcidin within the cell during maturation. Furthermore,...

Báo cáo khoa học: A novel gene, fad49, plays a crucial role in the immediate early stage of adipocyte differentiation via involvement in mitotic clonal expansion docx

... ORF of human fad49. We ﬁrst used the human genome data-base to predict the ORF region of human fad49 bysplicing out the introns and combining the exons of the ORF of human fad49. To isolate human ... SH3domain in the same protein, and that this intramolecu-lar interaction suppresses the lipid-binding activity of the PX domain in the resting state; phosphorylation of p47phoxreleases the binding, ... PXdomain to phosphoinositides might be regulated by the interaction between the PX domain and the third SH3domain, as in p47phox, although whether the interaction of the PX domain with the third...

Báo cáo khoa học: "An Online System for Corpus Management and Analysis in Support of Computing in the Humanities" pot

... handed over to the StorageHandler which decides how to store the resource.Since the output of the tagger is a XML documentit is stored as a XML Database. Finally the in- formation about the ... to perform PoStagging and has permission to read the document to be tagged. The next step is to fetch the docu-ment from the Storage Handler as input to the PoSTagger application module. The ... aspect of pure resourcemanagement, processing and analysis of docu-ments have traditionally been the domain of desk-top applications. Sometimes even to the point of command line tools. Therefore...

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... intoxication, diphtheria toxinis cleaved by furin into two fragments: the A chaincorresponding to the catalytic domain (C domain); and the B chain corresponding to the translocationdomain ... translocationdomain (T domain) and the receptor-binding domain. The C and T domains remain covalently linked by adisulﬁde bond. Following binding to its cell-surfacereceptor, diphtheria toxin is internalized ... of the pro-tein in the N state and the evolution of the overallstructure of the protein in the MG state. This degree of resolution is unprecedented for the T domain of the diphtheria toxin....

Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc

... applied to clarify whether the stoichiometry of the c rings is in uenced by variation of the expression level of subunit c. In the present study we investigated whether the c subunits from the sodium ... from the bottom of the cylinder and forms the central stalktogether with the e subunit. A striking feature of the structure is an inherent asymmetry among the catalyticb subunits. In combination ... that the size of the ring isnot changed by massive overexpression of subunit c in the E. coli host cells, indicating that intrinsic fea-tures of the monomeric unit determine the number of subunits...

Báo cáo khóa học: Binding analyses between Human PPARc–LBD and ligands Surface plasmon resonance biosensor assay correlating with circular dichroic spectroscopy determination and molecular docking ppt

... measure the bindinginteractions of small molecules to the ligand-bindingdomain (LBD) of human estrogen receptor [15]. To the best of our knowledge, there is to date no reportconcerning the ligand–PPAR ... between the SPR binding aﬃnities and Auto-Dock-predicted binding free energies.Table 3. The binding free energies of the ligands binding to PPARc. The binding free energies (kcalÆmol)1) of the ... deter-mination. It is interesting that the thermal stability of the receptor, reﬂected by the increase of the transition tem-perature (Tm), was enhanced by the binding of the ligands. The increment...

Báo cáo khoa học: GlnK effects complex formation between NifA and NifL in Klebsiella pneumoniae docx

... tetracycline-resistance cassette into the HindIII site. pRS239 was obtained by inserting the tetra-cycline-resistance cassette into the HindIII site of pRS155,encoding glnK under the control of t ... amajority of NifL was sequestered to the cytoplasmic mem-brane. These ﬁndings indicate that inhibition of NifA in the presence of oxygen and/ or ammonium occurs via directNifL interaction and formation ... Quantities of MBP-NifL and GlnK in the cytoplasmic and membrane fractions werecalculated as relative to total MBP-NifL and GlnK,respectively, setting the absolute amo unts of the respectiveprotein in...

Báo cáo khoa học: Ribonuclease H: properties, substrate speciﬁcity and roles in retroviral reverse transcription ppt

... but the larger b subunit, in addi-tion to possessing both the polymerase and RNase Hdomains found in the a subunit, also contains a C-ter-minal region corresponding to the viral integrase. The ... RNA strands in red and DNA strands in black. In the internal cleavage mode, the arrows mark the sites of cleavage along the length of the hybrid where nucleotide sequencealone determines the cleavage ... plus-strand initiation [8]. Underscoring the importance of this speciﬁc cleavage event is the fact that the initiationsite of the plus-strand DNA determines the left end of the linear product of reverse...

Tài liệu Báo cáo khoa học: Proteasome involvement in the degradation of the Gq family of Ga subunits pptx

... saline (NaCl ⁄ Pi) and chased for indicated times in DMEM containing an excess of cold methionine. To determine the effect of various pro-tease inhibitors, cells were treated with inhibitors ... degradation of the phosphoinositidaseC-linked guanine-nucleotide-binding protein Gqalpha ⁄ G11 alpha following activation of the human M1muscarinic acetylcholine receptor expressed in CHO cells. ... 7(A,B), no change in the total amount of protein or in the rate of degrada-tion was observed, which is an indication that the binding of Ga to Gbc subunits may not the limitingfactor for its stability.DiscussionIn...

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