Báo cáo khoa học: N-Methylation in polylegionaminic acid is associated with the phase-variable epitope of Legionella pneumophila serogroup 1 lipopolysaccharide pptx

... mAb LPS -1 bound only to LPS species lacking the mAb 2625 epitope, thus suggesting that the mAb 2625 epitope inte rferes with the binding of mAb LPS -1. The OPS of L. pneumophila Sg 1 is a polylegionaminic acid ... and the loss of the mAb 2625 epitope is one of them. Phase variation in L. pneumophila was f ound to in uence other LPS biosynthesis pathwaysinvolved in assembly of lipid A. Phase variant 811 ... a-D-Manp- (1 ® 8)-Kdo disaccharide in the inner core region and the structure of the complete core region of the Legionella pneumophila serogroup 1 lipopolysaccharide. Carbohydr. Res. 304, 91 95. 11 ....

Báo cáo khoa học: Differences in substrate speciﬁcities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes potx

... to be able to interpret the way is which this is achieved, but the results show the complexity of the interactions that occur both within the mature protein and in the acquisition of its tertiarystructure. In ... a C-terminaldomain of either 16 or 10 0 amino acids [7]. The ﬁrst twogenes of t he array, CPB1 and CPB2, are atypical becausethey encode enzymes with a C-terminal domain of just 16 amino a cids ... affectactivity.Abz-FRAK(Dnp)-NH2Abz-FRAK(Dnp)-OHpH3456780400800 12 00 16 00200024002800 10 9pH345678 91 0200400600800 10 00 12 00 14 00 16 00 18 0020000pH345678 910 0200400600800 10 00pH345678 910 020406080 10 0 12 0 14 0 16 0 18 0pH345678 910 04080 12 0 16 0200240pH345678 910 kcat/Km (mM -1 .s -1 )kcat/Km (mM -1 .s -1 )kcat/Km (mM -1 .s -1 )kcat/Km...

Báo cáo khoa học: Novel c-carboxyglutamic acid-containing peptides from the venom of Conus textile docx

... distribution of the peak atm ⁄ z ¼ 9 01. 18 indicates a bromine-containing peptide(Fig. 1A,B, inset). The peak at m ⁄ z ¼ 626.29 is consis-tent with amidation of the C-terminal fragment(DVVCS), as is the ... exhibits a 14 Da massincrease consistent with methylation of the side chaincarboxyl group of the N-terminal Asp residue conﬁrm-ing amidation of the C-terminus. Partial methylation of the internal ... from protein engineering of amino acid -4. Nucleic Acids Res 15 , 9505–9 513 .Supplementary material The following supplementary material is availableonline:Fig. S1. Puriﬁcation of conotoxins. (A)...

Báo cáo khoa học: "Optimization in Coreference Resolution Is Not Needed: A Nearly-Optimal Algorithm with Intensional Constraints" ppt

... constraints. One of the central global constraints in the ﬁeld of corefer-ence resolution evolves from the interplay of intra-sentential binding constraints and the transitivity of the anaphoric ... preceding 1 to 0. The costs of swapping a preceding 1 to 0 arehigher than the gain from swapping the 0 to 1 (asa consequence of the Balas ordering). So no solu-tion with the same 1- length can be ... of Zero-One ILP: the constraints come without anyweights. The reason for this lies in the logical na-ture of NLP constraints. For example in the case of coreference, we have the following types of con-straints: 1. ...

Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf

... [20].Extending this to mixtures of large proteins (seebelow), nozzle-skimmer dissociation spectra of 1 : 1, 2 : 1 and 3 : 1 mixtures of 14 4 and 11 6 kDa proteinsshowed the corresponding molecular ... after eliminating the mistakenly pre-dicted N-terminal Met. The fragment ions containing the C-terminus have the predicted mass values goingback 10 residues to Cys183, but after Cys180 they areall ... (C-terminal)-containing fragment ions. Thus, for the + 1. 0 Da sam-ple (Fig. 8B), the N-terminal fragment ions show littleincrease in mass with increasing size until Asn67, with this increase of...

Tài liệu Báo cáo khoa học: Oxidized elaﬁn and trappin poorly inhibit the elastolytic activity of neutrophil elastase and proteinase 3 pdf

... A (19 75) Pro-ceedings: Selective oxidation of methionine residues in proteins. Isr J Med Sci 11 , 11 71. 17 Matheson NR, Wong PS & Travis J (19 79) Enzymaticinactivation of human alpha -1- proteinase ... 3.0 ± 1. 0 · 10 )11 3.6 ± 0.5 · 10 6 1. 1 ± 0.2 · 10 )4NCS 9.2 ± 2.7 · 10 )9 1. 0 ± 0.3 · 10 69.0 ± 3 .1 · 10 )3MPO 5.8 ± 0.9 · 10 )9ND NDPr3 Elaﬁn None 1. 2 ± 0 .1 · 10 )10 3.3 ± 0.03 · 10 64.0 ... kass(M )1 Æs )1 ) kdiss(s )1 )NE Elaﬁn None 8.0 ± 0.5 · 10 )11 3.7 ± 0 .1 · 10 63.2 ± 0 .1 · 10 )4NCS 5.7 ± 0.6 · 10 )9 1. 1 ± 0.3 · 10 66.3 ± 0.6 · 10 )3MPO 4.0 ± 0.6 · 10 )9ND NDNE Trappin...

Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf

... removal of the NDA amino acid region abolishes proper nucleartargeting of the mSMOl isoform.ResultsStructural analysis and modeling of vertebrateSMO proteinsComparison of the derived amino acid ... has not been shownto contain any residue involved in either the catalyticsite or the FAD-binding domain [9 ,10 ,12 ,13 ]. Interest-ingly, this 31 amino acid region is highly conservedamong mammals ... mSMOa is cytosolic. The only structuraldifference between the two isoforms is the presence of an extra protein domain in mSMO l, encoded by the exon VIa [10 ].Comparative analysis of the amino acid...

Báo cáo khoa học: Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy pot

... O-chainLPSs of wild-type RC1, from the LPS of a s pontaneousphase variant (strain 811 ), and an isogenic mutant ( strain5 215 ) [15 ]. In the present study we investigated the binding of the antibody to the ... [5,9]. In L. pneumophila Sg 1 LPS the OPS is linked to a terminalnonreducingL-rhamnose (RhaII) of the core oligosaccharide [10 ,11 ]. The core of the LPS lacks heptose and phosphate,contains ... ZaÈhringer, U. (19 9 7)Identi®catio n of an a-D-Manp- (1 ® 8)-K do disacch aride in the inner core region and the structure of the complete core region of the Legionella pneumophila s erogr oup 1...

Báo cáo khoa học: Nautilin-63, a novel acidic glycoprotein from the shell nacre of Nautilus macromphalus doc

... proteinFEBS Journal 278 (2 011 ) 211 7– 213 0 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 212 1These results indicate that N63 interacts obviously with the precipitation of CaCO3because it inducesdrastic ... gel.Nautilin-63, a novel shell nacre protein B. Marie et al. 212 0 FEBS Journal 278 (2 011 ) 211 7– 213 0 ª 2 011 The Authors Journal compilation ª 2 011 FEBSdouble staining allowed visualization of most of the macromolecular ... 2 011 )doi :10 .11 11/ j .17 42-4658.2 011 .0 812 9.x In molluscs, and more generally in metazoan organisms, the production of a calciﬁed skeleton is a complex molecular process that is regulated by the secretion...

Tài liệu Báo cáo khoa học: A single amino acid substitution of Leu130Ile in snake DNases I contributes to the acquisition of thermal stability A clue to the molecular evolutionary mechanism from cold-blooded to warm-blooded vertebrates docx

... susceptible. These ﬁndings show that the presence of Val67 is one of the essential factors responsible for the actin-binding capacity of these snake DNases I.Tissue distribution of snake DNases I The ... be involved in changing the thermally stable characteristics of vertebrateDNases I, substitution of Ile130Leu in snakes and insertion of Ser205 in amphibia. It is interesting that DNases I of warm-blooded ... [4,5 ,15 ,17 ,19 ]. When the primary structures of the three snake enzymes were compared with those of othervertebrates, only two aa residues, Leu130 and Leu166, of Fig. 2. Alignment of the amino acid...

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