... Antibacterial peptides in stimulated human granulocytes
Characterization of ubiquitinated histone H1A
Yuqin Wang, William J. Grif®ths, Hans JoÈ rnvall, ... ubiquitination of
histone H1Ausing electrospray ( ES) MS. The tripeptide A rg-
Gly-Gly, corresponding to the three C-terminal residues of
ubiquitin, was found to be linked to Lys222 of histone H1A.
MATERIA...
... composed
of EIIA, B and C domains, with the EIIA and B do-
mains being part of the phosphorylation chain and the
EIIC domain representing the membrane domain. As
all components of the PTS, depending ... phosphory-
lation of glucose and a number of other carbohydrates
(Fig. 1). It consists of two common cytoplasmic pro-
teins, enzyme I (EI) and histidine-containing protein
(HPr), a...
... The binding of ZIP to Grb14, by
recruiting protein kinase Cf, enhances the serine phos-
phorylation of Grb14 and its inhibitory effect on IR
kinase and insulin signaling [10]. The interaction of
Grb14 ... tyrosine phosphorylation
in both activation and inhibition of the insulin recep-
tor tyrosine kinase in vivo. Endocrinology 137, 4960–
4968.
24 Kelly KL & Ruderman NB (1993)...
... (gradient of 15–150 mm NaCl contained
in 1 L buffer A) to obtain pure protein.
Redox potentiometry, ligand binding and
stopped-flow kinetic analyses
Redox titrations and the determination of redox ... 6-S-cysteinyl flavin mononucleotide-containing
histamine dehydrogenase from Nocardioides simplex:
molecular cloning, sequencing, overexpression, and
characterization of redox centers of...
... an increase in binding capacity
[25].
Identification of nitration site in the extracellular
domain of ILT2
In the extracellular domain of ILT2, there are several
Tyr residues that participate in ... results
indicate that NO maintains, or even increases, ILT2-
mediated suppression in NKL cells.
After HLA-G binding, immunoreceptor tyrosine-
based inhibitory motifs in the cytopl...
... We investi-
gated the possible involvement of Vfr in the action
of natriuretic peptides on P. aeruginosa, using strain
PAO9002, a vfr null mutant of P. aeruginosa PAO1
obtained by insertion of ... intrabacterial
concentrations of cAMP and cGMP. The concentra-
tion of cAMP in P. aeruginosa PAO1 grown in ordin-
ary nutrient broth medium (BNO) in the absence of
treatment...
... to determining the functional orga-
nization of the human parser. In Section l, a pre-
liminary taxonomy of parsing models is presented,
based upon a consideration of modes of handling
ambiguities; ... so
far. Instead of the parser, after making a single
or parallel analysis of an ambiguity, maintaining
the analysis/es as further input is received, one
can imagine it j...
... resulted in
enhanced integrin clustering and activation of a
5
b
1
transcription by protein kinase C signaling, which, in
turn, upregulated cell surface expression of a
5
b
1
,
resulting in increased ... function
Integrin sugar chains play important roles in the
biological functions of integrins
A growing body of evidence indicates that the presence
of the appropriate oligos...
... folding
of an enzyme. I. Theory of protein engineering anaylsis of sta-
bility and pathway of protein folding. J. Mol. Biol. 224, 771–782.
21. Clackson, T. & Wells, J.A. (1995) A hot spot of ... of exacting structural
criteria; only properly folded proteins are active. Protein
folding is dictated by amino acid sequence [12]. In an
ensemble of proteins composed from the sta...
... successful metabolic engineering
of freezing tolerance in plants should include such an
analysis of the dynamics of metabolism to gain com-
prehensive information about the effects of individual
overexpression ... the integral of carbon uptake per
hour. Data points were spline-interpolated to obtain time-
continuous information on net photosynthesis during the
whole period of co...