... for 5min. The amount of protein of the solubilizedcells was determined with a bicinchoninic acid protein assaykit (PIERCE, Rockford, IL, USA) using bovine serumalbumin as a standard. The solubilized ... cells and human umbilicalvein endothelial cells [20–23], the mechanism of the TS effectstill remains unclear. Therefore, we investigated in detail the effect of TS on NO production in the VSMC ... suggestingthat TS participates in the LPS-stimulated signal pathwayleading to NO production. Protein kinase C (PKC)inhibitors, but not protein kinase A inhibitors, inhibited the enhancing effect of...