ANTIBIOTIC RESISTANCE OF ESCHERICHIA COLI ISOLATED FORM POULTRY WORKERS, PATIENTS AND CHICKEN IN THE EASTERN PROVINCE OF SAUDI ARABIA ppt
ANTIBIOTIC RESISTANCE OF ESCHERICHIA COLI ISOLATED FORM POULTRY WORKERS, PATIENTS AND CHICKEN IN THE EASTERN PROVINCE OF SAUDI ARABIA ppt
... investigate and compare the patterns of resistance of E. coli isolated from poultry industry workers, patients and chicken, and to identify the common serotypes of E. coli isolated from humans and poultry. Materials ... demonstrate alarmingly highindividual and multiple resistance to antibiotics in E. coli, reflecting the misuse of these agents in the poultry industry.Since chicken may act as a possible source of pathogenicorganisms ... pattern of E. coli isolated from poultry industry workers and E. coli isolated from chicken (Table 2). However, despite the similarity of resistance patterns to the tested antibiotics in E. coli- chicken...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... the sequences of 1CEV and 1RLA and pasted into the structural alignment. The alignment was then corrected byhand. Since the sequences of the two arginases and agmatinase greately differ in the ... agmatinase with the GfiAandCfiT substi-tutions in the coding and noncoding strands, respectively, and using the 5¢ and 3¢ primers mentioned above, the fulllength agmatinase cDNA coding for the ... arginase (yellow) and the modelled structure of E. coli agmatinase (blue). Note the shorter extension, in the case of E. coli agmatinase, of the loop indicated by the letter a.Fig. 5. Scheme of...
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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
... prerequisitefor the accessibility of the inner core sugars for the bindingbymAbWN1222-5.Previously, an NOE between the anomeric proton of the side-chain heptose (residue H) and the axial H-3 of the first ... (strong) and H-3e (very weak) of a-Kdop(residue C) and H-1 of L,D-Hepp (residue H) and between the anomeric proton of the innerL,D-Hepp residue (E) and the equatorial H-3 of the side-chain ... J.A., Heinrichs, D.E., Monteiro, M.A., Perry, M.B. &Whitfield, C. (1998) Involvement of waaY, waaQ, and waaP in the modification of Escherichia coli lipopolysaccharide and their role in the formation...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... of R120 by lysine reduces the probability of forming the fully closed form of the protein. The results obtained with the K22V/R120K doublemutant protein point toward a central role of arginine ... two other positively charged amino acid sidechains, R397 and R120, are also involved in fosfomycinbinding, and in view of the multitude of interactions, it wasassumed that deletion of a single ... with the K22V and K22E mutantproteins. This was interpreted as a lack of fosfomycinbinding to these mutant proteins [12]. Binding of fosfomycinto either wild-type MurA or the K22 mutant proteins...
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Báo cáo khoa học: Role of DptE and DptF in the lipidation reaction of daptomycin ppt
... is GLDSV,indicating that this putative ACP domain is one of the few ACPs in which valine replaces isoleucine (I) orleucine (L) in the conserved sequence. To determinewhether DptF is the putative ... of DptE, weexpressed dptF using the pQTev vector in E. coli and purified the resulting ACP as an N-terminal His7 fusionprotein (Fig. 3) with a yield of 9.5 mgÆL)1 of culture. The identity of ... [37].Interestingly, in the case of the lipopeptide surfactin,neither an acyl CoASH ligase-like domain nor an ACPcould be identified within the biosynthetic gene clusterusing bioinformatic tools...
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Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx
... of the trypsin-sensitiveregion situated in the linker domain of Abcb1a. Fol-lowing trapping in the absence or presence of Vi and mild-trypsin treatment, the trypsin degradation pro-ducts of ... trapping isalmost normal. By contrast, when the charge isremoved, as in the glutamine (length of the side chainmaintained) and asparagine (shorter side chain)mutants, then trapping in the ... corresponding to the N- and C-terminal halves of the protein cut at the linker region could also be detected in lanes 2–4 ()Vi and +Vi) and the radiolabel could be detected in eachfragment, both in the...
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Báo cáo khoa học: "Some Comments on Algorithm and Grammar in the Automatic Parsing of Natural Languages" ppt
... written in the form of appropriate tables of rules. The paper raises the question of the increasing complexity of the tables when more than the most elementary natural-language con- ditions are included, ... Examples of this are very common in English and include many of the frequently cited instances of nesting. The second consideration is that the determination of external functioning by con- ... attention to the parsing problems encoun- tered in machine translation. It must be noted at the outset that in this author’s opinion the aim of the automatic-parsing component of a machine-translation...
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Tài liệu Báo cáo khoa học: Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases pdf
... higherthan for the THF:A ligand. The presence of Mg2+hadlittle effect on the binding of Fpg to the THF:C ligand and slightly improved binding to the THF:A ligand,making it comparable with binding to ... decrease in binding at 0 and 500 mm KCl [32]. Thus, to address the in uence of the reaction conditions on the binding of Fpg and OGG1to damaged DNA, we determined Kdvalues for bindingunder the ... proceed for 5 min (Fpg) or 10 min (OGG1). In the case of Fpg, the reaction was terminated by mixingwith 1 ⁄ 2 vol. of formamide loading dye [71] and heatingfor 5 min at 95 °C. To measure the glycosylase...
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Tài liệu Báo cáo khóa học: The C-terminal domain of Escherichia coli Hfq increases the stability of the hexamer ppt
... between the N-terminaldomain of Hfq and the Sm and Sm-like (L-Sm) proteins of eukaryotes and archaea [11,15,16]. These proteins arecomponents of the spliceosome complex and are alsoinvolved in other ... structure. The absence of this domain results in a reduction of the b-sheet characterwithin the Sm domain and could perturb the hydrogenbonding interactions between the interchain strands.Indeed, ... nucleic acid bindingprotein involved in the synthesis of many polypeptides.Hfq particularly affects the translation and the stability of several RNAs. In an earlier study, the use of fold recog-nition...
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Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf
... retardation band, which indicatescomplex formation between DNA duplex II and the Fpgprotein. The intensity of the retardation band increasedwith increasing Fpg protein concentration. The bindingreaction ... duplexesE. coli Fpg protein recognizes a hexanucleotide sequencewith oxoG in the middle in the lesion-bearing DNA strand and specifically binds to it and the oxoG-pairing residue of the other strand ... stacking interaction with the aromatic ring of the TFMDPh group. The complementary DNA strandforms few interactions with the enzyme, and the amino acidresidues involved are not conserved [11]. The...
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