Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot
... 5¢-TCATGCTGGTTGAGACTG
GA-3¢ and 5¢-CCAGGTGTGGAGCAAGGCAG-3¢) and a
938 bp fragment of promoter 3 (primers, 5¢-CTCACAG
CAGCCAGTAAGTG-3¢ and 5¢-ACTCACAGGCTCTGG
GGCTG-3¢) were amplified using a Taq polymerase ... 5¢-GGACTCAAGAGAGGAACCTG-3¢; F1b, 5¢-CT
GCCTTGCTCCACACCTG-3¢; F1c, 5¢-CTGCCGTCTCC
GCCGCCACT-3¢; FU, 5¢-TCAAGCCCCGCTACATAG
TT-3¢; and R3, 5¢-AGGAACCAAACACCAAGTGG-3¢
(Fig. 2A)...
... of
the associated polymerase domain. In the internal
cleavage mode, the RNases H behave as typical endo-
nucleases and cleave the RNA along the length of a
DNA ⁄ RNA hybrid substrate in the absence ... retroviral RNases H. DNA ⁄ RNA
hybrids are drawn with RNA strands in red and DNA strands in
black. In the internal cleavage mode, the arrows mark the sites of
cle...
... ectodomains, C-terminal fragments and
intracellular domains (ICDs). One of the proteases that is known to cleave
APP and that is essential for generation of the amyloid b-protein is the
b-site APP-cleaving ... that the APP
family of proteins have a role in cell–cell and cell–
matrix adhesion, and that they can form both cis and
trans homodimers and heterodimers...
... hamster ovary cells. Hybridoma
(Larchmt) 26, 1–9.
9 Ismaili A, Jalali-Javaran M, Rasaee MJ, Rahbarizadeh
F, Forouzandeh-Moghadam M & Memari HR (2007)
Production and characterization of anti-(mucin
MUC1) ... sev-
eral groups have reported the isolation of single-
domain antibodies (sdAbs) consisting of the variable
domain of these heavy chain antibodies, also named
VHH [...
... min at 30 °C, stopped by the addition of
SDS/PAGE loading buffer, and analyzed on SDS/PAGE
gels and by autoradiography. The separation of substrates
from kinases after the kinase reaction was carried ... 1009
lanes with samples that contained no substrate. After
preparing a graph of the intensity of the signal from the
substrates only, we calculated the percentage...
... of a
word into other languages found in parallel cor-
pora are seen as the (translational) context of that
word. We assume that words that share transla-
tional contexts are semantically related. ... >
1.
newspaper text (Algemeen Dagblad and NRC
Handelsblad 1994/1995). The corpus was parsed
automatically using the Alpino parser (van der
Beek et al., 2002; Malouf and van Noo...
... II and III, the only physical connec-
tion between domains G and II and domains III, IV
and V. In the S. aureus EF-G structure, this loop has
a bent conformation, and packs against domain III
and ... 666 and 670, within one b-strand of
domain V, are also important for interdomain interac-
tions. The N-terminal part of the strand interacts with
the N-termina...
... complexes
The S, Zn, Cd and Cu content of the Zn–, Cd– and
Cu–CeMT preparations was analyzed by means of ICP-
AES in a Polyscan 61E (Thermo Jarrell Ash, Franklin,
MA, USA) spectrometer, measuring S at ... residue, the wild-type cDNA was
amplified using the same upstream primer as for the entire
cDNA (5¢-CGGGGATCCATGGTCTGCAAG-3¢) but
5¢-ACGCGTCGACCTAAGCAGCCTG-3¢ as the dow...