... enhancing the conformational change in path C,and enabling a conformational change in path A, therebyincreasing the rate of both cycles. In reaction path A, binding of Mg2+ to subunit 2 induces a ... ligand can be an amino acidside-chain from the active site region, leading to homo-dimer asymmetry. It has been established that Ser102, the amino acid acting as a primary nucleophile in the activesite ... Dimer asymmetry and the catalytic cycle of alkaline phosphatasefromEscherichia coliStjepan Orhanovic´and Maja Pavela-VrancˇicˇDepartment of Chemistry, Faculty of Natural Sciences, Mathematics...