... was initiated
by introducing pyruvate kinase.
Fluorescence experiments
Fluorescence emission spectra of pyruvate kinase in 100%
water and in various water/dimethylsulfoxide mixtures were
determined ... activities of pyruvate kinase with Na
+
and K
+
could be related to distinct structural features of the
enzyme. Therefore, we determined the intrinsic fluorescence...
... generated, making multiple isoforms in combi-
nation [4]. Among these isoforms, DNp63a is the
predominant isoform expressed during embryogenesis
and in adult epidermal tissues, and is responsible for
epidermal ... degradation of DNp63a by
TNF-a was attenuated by inhibition of NF-jB, sug-
gesting that activation of NF-jB may be involved in
the regulation of the degradation...
... affected in a
similar way. Electrostatic interactions play a critical
role in guiding and stabilizing functional protein–pro-
tein interactions [6], as well as in the structural stability
of proteins ... provided important insights into the role of
heme–protein interactions for the structural stability of
this class of proteins.
The mutant D72K of cytochrome c
6
from Nosto...
... cytoplasmic mem-
brane. These findings indicate that inhibition of NifA in the
presence of oxygen and/ or ammonium occurs via direct
NifL interaction and formation of those inhibitory NifL–
NifA complexes ... tetracycline-resistance cassette into the
HindIII site. pRS239 was obtained by inserting the tetra-
cycline-resistance cassette into the HindIII site of pRS155,
encoding glnK...
... Westrick RJ & Ginsburg
D (1998) Comparative mapping of distal murine chro-
mosome 11 and human 17q21.3 in a region containing
a modifying locus for murine plasma von Willebrand
factor level. ... encodes a
region of the protein that is known to be essential for
kinase activity and contains a diacylglycerol kinase- like
catalytic domain [2]. As shown in Fig. 5A, cell deat...
... membrane expansion
and remodeling. In most instances, there is coordinate
expression of the E1 and E2 components. The transient
accumulation of mRNA preceding changes in protein levels
and catalytic ... expression of mtPDC among dicot
plants. In a detailed study of pea (Pisum sativum) seedling
development, it was observed that changes in the levels of
E1 and E2 prote...
... measurements of the
interaction of IVIg with C-reactive protein (CRP). The
binding profiles obtained after single injections of
native and of low-pH buffer-exposed IVIg were com-
pared. As shown in Fig. ... Increased binding of low-pH buffer-exposed IVIg to human factor H. In both panels, binding
of the native IVIg is indicated by a solid line, and that of the low-pH buff...
... 3729–3734.
Supporting information
The following supplementary material is available:
Fig. S1. Amino acid alignment of linker region and
ligand-binding domain (LBD) of ecdysone receptors.
Fig. S2. Amino acid ... nonsteroidal agonists
bind exclusively to the EcR ligand-binding domain
(LBD), although the presence of USP increases
ligand-binding affinity [12–15].
The diversity of ligand-...
... FEBS
analysis of purine-binding proteins in a total lysate of
human Jurkat T-cells [40].
COFRADIC analysis of protein
processing ) protease degradomics
Protein processing introduces novel protein fragments
that ... light
12
C
6
-arginine
form. Almost identical numbers of pre-existing N-ter-
mini (i.e. the N-termini of intact proteins), on the
other hand, should appear as couples...
... °C, and carried
out for 32 cycles of 94 °C for 30 s, annealing temperature for
1 min and 30 s and 72 °C for 30–90 s. Products were visual-
ized by ethidium bromide in agarose gels, and bands ... the main nitrogen compounds in the
phloem exudates, amounting to 51% of the total
amino acids in the light and 56% in the dark
(Fig. 7A). The total amount of alanine, s...