Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp no 92 pdf

Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

... oligopeptide sequences and using PCR, the gene of the cyclodextrinase from Flavobacterium sp. no. 92 wasderivedfromthegenomicDNA.Thegenewas sequenced and expressed in Escherichia coli; the gene pro- duct ... longer and more complex than the loops at the opposite barrel end. The lengths of the b-strands in the barrel vary from two (b14) to seven (b11) resi...
Ngày tải lên : 08/03/2014, 02:20
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... II. The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). The two ... atoms, whereas the side chain of Lys173 interacts with both O6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the...
Ngày tải lên : 15/02/2014, 01:20
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Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... [57]. Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the nucleotide sequence from 89 254 to 89 667 in the Synechocystis ... above and in [1]). We investigated representative gene products from the E. coli and Synechocystis sp. bacteria further, to gain insight into the funct...
Ngày tải lên : 19/02/2014, 17:20
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Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc

Tài liệu Báo cáo khoa học: Covalent binding to glutathione of the DNA-alkylating antitumor agent, S23906-1 doc

... GSH and to the diol, mono-acetate and di-acetate forms of the parent drug, respectively. The latter three species reflect the hydrolysis of the compound in solution (see the results below with the ... reactivity of S23906-1 towards the bionucleophiles DNA and GSH. The covalent binding of the drug to GSH reduced the formation of DNA adducts and slightly...
Ngày tải lên : 20/02/2014, 11:20
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Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx

... distortion of the )1 sugar and by increasing the pK a of the catalytic acid. The hydroxyl group of Tyr214 makes a major contribution to the positioning of the N-acetyl group of the )1sugar.Taken together, ... simplicity, and in line with the results from the other calculations, the <0.0 value is allocated to Asp140 and > 20.0 to Asp142. b The calc...
Ngày tải lên : 07/03/2014, 14:20
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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... 7). Although the spectral characteristics of the lac1 protein suggest the absence of a type 1 copper site, this is not borne out by analysis of the deduced amino acid sequence of the enzyme. Thus, the ... amino acid sequence from Ala25 to Asp41 corresponded to the N-terminal sequence of the purifiedprotein,andtheputativepresequenceof 24amino acids is a hydrophobic s...
Ngày tải lên : 07/03/2014, 15:20
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Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

Báo cáo khoa học: Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p pptx

... comprising the N-terminal fragment and the first AAA-cassette of PEX6 (PEX6N + D1–GAL4-BD). Neither of the cas- settes alone nor the two AAA-cassettes together or the N-terminal part together with the ... assigned their corresponding binding sites and elucidated the importance of ATP-binding and -hydrolysis of Pex1p and Pex6p for their interaction. We show that t...
Ngày tải lên : 07/03/2014, 16:20
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Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx

Báo cáo khoa học: Spectroscopic and kinetic properties of the horseradish peroxidase mutant T171S Evidence for selective effects on the reduced state of the enzyme potx

... from comparison of the electronic absorption and RR spectra of the wild type and Thr171Ser either in the presence of the aromatic donor BHA or at alkaline pH. Addition of saturating amounts of ... amounts of BHA did not reveal any spectral differences between the wild type and the mutant (data not shown). Furthermore, the electronic absorption spectra of t...
Ngày tải lên : 07/03/2014, 21:20
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Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt

Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt

... vector. The nucleotides are numbered from the translation start site (ATG). The effect of the wild-type and mutants was measured as the mean of the firefly luciferase levels nor- malized by the Renilla ... conjunction with the luciferase gene in pGL3- basic vector. The nucleotides are numbered from the translation start site. The effect of the wild-type and...
Ngày tải lên : 15/03/2014, 10:20
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Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf

Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf

... Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B). The structures of bound sugars and the side-chains of three acidic residues in the conserved ... causes the distortion and twist- ing of the substrate, so that the glycosidic oxygen faces towards the bottom of the deep cleft. Enzyme–substrate interactions...
Ngày tải lên : 15/03/2014, 11:20
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