Báo cáo khoa học: "Mapping between Compositional Semantic Representations and Lexical Semantic Resources: Towards Accurate Deep Semantic Parsing" docx

... Computational LinguisticsMapping between Compositional Semantic Representations and Lexical Semantic Resources: Towards Accurate Deep Semantic ParsingSergio Roa†‡, Valia Kordoni† and Yi Zhang†Dept. of ... mapping between deep se-mantic representations and lexical semantic resources. A probabilistic model comprisingMinimal Recursion Semantics (MRS) struc-tures and lexicalist oriented semantic ... ap-proach towards mapping between deep and shallow semantic representations. More speciﬁcally, we useBayesian networks to acquire a statistical model thatenriches the Minimal Recursion Semantics...

Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

... Tullio, Enrico Defranchi, Franca Salamino,Edon Melloni and Sandro PontremoliDepartment of Experimental Medicine (DIMES), Section of Biochemistry and Centre of Excellence for Biomedical Research (CEBR),University ... calpain ligands and may accordingly induce changes in the conformationof the protease detectable by the mAb binding. Toinvestigate this, we exposed immobilized calpain todigestible and nondigestible ... After saturation,mAb 56.3 was added and the binding of the mAb to calpain wasmeasured as described in Experimental procedures and the legendto Fig. 1B and expressed as arbitrary units. Alternatively,...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... xenotoxins. At the blood–brainbarrier, and in placental trophoblasts, testis and bonemarrow, it provides protection of vital body parts and, in the gut, liver and kidney, Pgp helps to eliminate ... [15–18]. On the other hand, MRP1is present in virtually all human tissues and in mosthuman tumor cell lines and tumor samples [4]. Usingmice genetically deﬁcient in both the mdr1a and mdr1bgenes ... lM dye and 20 lM NBD-Cl (trian-gles) or 5 lM dye and 50 lM MK571 (squares). Another cell sample was depleted of ATP by incubation in a medium containing CCCP and 10 mM deoxyglucose and in...

Báo cáo khoa học: Interactions between coenzyme B12 analogs and adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum pot

... coenzyme-binding and kinetic studies of AdoCbl analogs withglutamate mutase.ResultsSynthesis of MeCbi, AdoCbi and AdoCbi-GDPMeCbi and AdoCbi were successfully separated fromunreacted MeCbl and AdoCbl and ... 50, 75 and 120 lm cofactor, and the Km and kcatfor AdoCbi-GDPwere determined in the presence of 20, 70, 100, 150 and 200 lm cofactor. The reaction was initiated by addingl-glutamate and incubating ... molecular masses of MeCbi and AdoCbidetermined by ESI-MS were 1004.5 and 1240, whichcompare favorably with calculated relative molecularmasses for MeCbi and AdoCbi of 1004.1 and 1239.6,respectively....

Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx

... differ at exon 9 (and atthe N-terminal exons 1 and 2). Thus comparison between skeletal and smTm isoforms does not allowthe assignment of the transitions of smTm.Comparison between the three ... signiﬁcantly increasesthe thermal stability and unfolding enthalpy of Tm, and reduces the difference in total enthalpy seen between smTm and nonmuscle Tm isoforms 5a and 5b(Fig. 7, Table 2). In particular ... strong and weak sides, but combination of both the DSC and CDprovides a powerful approach for structural characteri-zation of Tm and its interaction with actin.In this work we have used DSC and...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... compilation ª 2011 FEBS 1661Interaction between very-KIND Ras guanine exchangefactor and microtubule-associated protein 2, and its rolein dendrite growth – structure and function of the secondkinase ... dendrites and soma, although not to axons. The expression of threeother KIND2-containing constructs (DKIND1, DRasN and DGEF) was restricted to dendrites and soma(Fig. 1B). On the other hand, two ... mousev-KIND KIND1, Spire-2 KIND and PTPN13 KIND(Fig. 6A). The amino acid sequences of the coreregions of mouse, human and Gallus v-KIND shared65.6% homology, and 17 identical and four function-ally...

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... citrate and 0.1Mcysteine hydrochloride, in short, the resolution buffer.Various pH values between 5.0 and 8.0 were tested, and apH of 7.0 was chosen (see below). Prior to the resolution,CcStP and ... potassium phosphate between < 0.05 and 80 mM. PLP at a concentration of between 0.0 and 100 lMwas added to reconstitute the holo-enzyme. Thereaction was carried out at 30 °C and typically, ... subunitinteractions towards t he induction of full enzymatic activity and optimum stability in a dimer of phosphorylase. This is asigniﬁcant and central problem to the study of catalysis byGPs and oligomeric...

Tài liệu Báo cáo khoa học: Mapping the functional domains of human transcobalamin using monoclonal antibodies pptx

... Assembly of two domains achieved thecomposite structure of the ligand-binding site and built the compatible interface between IF and itsreceptor [14]. The domain organization of TCremains unknown ... peptides TCy46,TCy37 and TCy31. How-ever, among the group 1 mAbs, only 3C12 and TC2bound to all the fragments, whereas 2-2 and 4-7 didnot recognize TCy31(Fig. 4A, lanes 2-2 and 4-7).Interaction ... fragments 4 and 10–11, respectively. The synthetic peptides were testedfor binding to mAbs 2-2, 3-9, 3C12, 4-7 and Q2-2, and the reaction was observed for two combinations(PA+ mAb 2-2) and (PB+...

Tài liệu Báo cáo khoa học: Mapping of the epitope of a monoclonal antibody protecting plasminogen activator inhibitor-1 against inactivating agents pptx

... onb-sheet A and the ﬂexible joint region. Curiously, the epitopeis localized in a-helix C and the loop connecting a-helix I and b-strand 5A, on the side of PAI-1 opposite to b-sheet A and distantly ... ± 0.5E55/71A-Q58/74A hC K and R >20 000a1.6 ± 0.3E55/71A-D307/318A hC and hI/s5A loop K and D >20 000a3.6 ± 1.1Q58/74A-D307/318A hC and hI/s5A loop R and D >20 000a2.6 ± 0.5aaSigniﬁcantly ... ofXR5118 and bis-ANS used in these assays were 250 lM and 80 lM, respectively, due to solubility limits.Measurements of the effects of Mab-1 and VN onPAI-1 latency transition and TVASS...

Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf

... cells and ﬁbroblasts were labelled with [35S]sulfate, washed and incubated with chondroitinase ABC and HS lyase. Triton extractsof untreated and enzymatic treated cells were prepared and analysedby ... 0.01MN-ethylmaleimide, 1% Triton X-100 and 5 lgÆmL)1ovalbumin. Extracts were precipitated with threevolumes of 95% ethanol and 0.4% sodium acetate and werethen dissolved in SDS sample buffer and analysed bygradient ... experiments with unlabelled GAGs. Asexpected, unlabelled DS and HS6 (and heparin) eﬃcientlyblocked the interaction between 125I-labelled DS and AP1,whereas unlabelled CS did not (Fig. 1C). Moreover,...

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