... and glycine; 0.25 mgÆmL)1L-aspartic acid;0.1 mgÆmL)1L-asparagine,L-histidine,L-isoleucine,L-lysine,L-proline,L-threonine,L-tyrosine and L-valine;0.25 mgÆmL)1L-methionine; ... mgÆmL)1FeCl2;0.05mgÆmL)1nicotinic acid;0.05 mgÆmL)1thiamin; 0.1 lgÆmL)1biotin; 0.125 mgÆmL)1guanosine, cytosine and uracil; 0.08 mgÆmL)1thymine;0.4 mgÆmL)1L-alanine,L-glutamic acid,L-glutamine,L-arginine ... side chains of theresidues around the active site and bind in their proximity, in uencing the intensity of the fluorescence of the trypto-phan residues and resulting in a fluorescence quenchingeffect.Fig....