Báo cáo khoa học: "Some Problems in the Mechanical Translation of German" pdf

... per cent of the instances the subject, in 25 per cent of the instances the direct object, and in 45 per cent of the instances an independ- ent prepositional phrase. In the remaining 10 per ... the feminine singular dative. 2. The masculine singular accusative of wel- cher is identical to the dative plural. 3. The nominative form is identical to the accusative in the feminine ... excludes the possibility of it being nominative, then the relative pronoun may be taken to be the subject of the clause. This ap- plies to about half of all the instances where the function of the...

Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf

... productive binding to the substrate via twoelements in ClpA, one in the N-domain and the other in the pore of the ClpA hexamer. In the case of shortunstructured peptides or unfolded proteins such ... debate regarding the role of the ClpBN-domain (which shares a common fold with the N-domain of ClpA) in substrate selection [15–17]. Onedifﬁculty in understanding the role of the N-domain of ClpA ... residues in the N-domain of ClpA by monitor-ing the binding, unfolding and degradation of several different substrates,including short unstructured peptides, tagged and untagged proteins. Inter-estingly,...

Báo cáo khoa học: An arginyl in the N-terminus of the V1a vasopressin receptor is part of the conformational switch controlling activation by agonist docx

... construct in which the N-terminus of the OTR is replaced with the corresponding sequence of the V1aR [10]. The role of the N-terminus has beenaddressed recently by alanine-scanning mutagenesis of the N-terminus ... off-state. The unique ability of arginyl to fulﬁl this constraining function in the N-terminus of the V1aR is similar to the unique constraining role of alanyl previously reported for Ala293 in the ... stabilize the G-proteininteraction of the active receptor [28]. In summary, Arg46 of the V1aR is required for highafﬁnity agonist binding and signalling. It is one of the constraining residues...

Báo cáo khoa học: Local changes in the catalytic site of mammalian histidine decarboxylase can affect its global conformation and stability pptx

... inhibitor was not ableto protect the enzyme against thermal denaturation. The increased resistance of the a-FMH-treated protein tothermal denaturation would indicate that the covalentbinding ... at the interface between the monomers (Fig. 1), as is the case of DDC [18]. One of the monomers (monomer A) would contain the major part of the catalytic site pocket, including K308 and H197 in ... how long the enamine remains in the active site and its rate of attack on the aldimine bond,and can be modiﬁed by slight differences in the position of the residues [41]. As covalent binding was...

Báo cáo khoa học: Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations pot

... cataloging the structure to class I. As the bulky NH2group on guanine was believedto prevent binding of the drug in the minor groove, the detailed nature of several of the amidinium and guanidinium ... sequence of the decamer. Part of the spine of hydration is substitutedby the amide N atoms of the minor groove binder.As the crystal engineering technique used resulted in a 1.75 A˚resolution, the ... (not shown). In uence on the minor groove widthAs reported previously [28,29], one of the major conse-quences of binding of a drug molecule in the minorgroove is the widening of this groove,...

Báo cáo khoa học: "A Program for the Machine Translation of Natural Languages" pdf

... translated—whether the indicated juxta- position of the elements (in either order) is a correct translation of the corresponding part of the input. That is, he must think of the corresponding part of the ... supplies the machine with the part of the input which led to the translation together with the association of the units in and indicates for each unit of the input text to which units of ... set of states F of A, the set of derived functions fa of f, letting f itself be the initial state. The input I of A is the semigroup on which f is defined, and the output O is the range of...

Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression proﬁling ppt

... presence of 20E,however, favors the formation of the AaEcR⁄ AaUSPheterodimer [13], indicating that the regulation of the activity of these proteins occurs through protein–pro-tein interactions, ... single protein. Interestingly, during the initialprocess of identifying the NR family members in the A. aegypti genome, we predicted two isoforms of AaFTZ-F1, differing only in their A ⁄ B region, ... Thisamino acid induced signal requires protein translation, as demonstrated by the lack of upregulation of AaHNF-4A in the presence of Chx (Fig. 4B). Address-ing the question of the nature of this...

Tài liệu Báo cáo khoa học: Electrostatic contacts in the activator protein-1 coiled coil enhance stability predominantly by decreasing the unfolding rate docx

... interpretation in distinguish-ing interacting from noninteracting proteins. OneFig. 1. Schematics of library designs. The helical wheel diagram looks down the axis from the N-terminus to the C-terminus. ... thisoverall value into its component kinetic steps. The ﬁndings of this study are therefore of interest to the protein design ﬁeld in general, but also inform uponhow to fast track the design of peptides ... of using peptides as the starting point in the generation of therapeutics is now a growing area[18,19]. Peptides harbour the potential for chemicaland biological diversity while maintaining...

Tài liệu Báo cáo khoa học: Final steps in the catabolism of nicotine Deamination versus demethylation of c-N-methylaminobutyrate doc

... to the complete mineraliza-tion of the pyrrolidine ring of nicotine, which afteroxidation by 6-hydroxy-l-nicotine oxidase, is cleavedoff from the pyridine ring of nicotine in the form of CH3-4-aminobutyrate. ... expression of these genesdepends on the presence of nicotine in the growthmedium and we have determined the enzyme activities of the proteins. Our results demonstrate the presence of two pathways of ... to other members of the polyamine oxidases, the FAD cofactor was noncova-lently bound to the apoprotein and the C-terminalﬁngerprint sequence SGGCY of monoamine oxidases,with C being the cysteine...

Tài liệu Báo cáo khoa học: Isochore structures in the chicken genome ppt

... versa, indicating a mosaic structure of the chromosomes. A jump in the Àz0ncurve indicates an increase of the G + C con-tent; whereas a drop down in the Àz0ncurve indicates a decrease of the ... the upperpanel shows the cumulative percentage of the values at or beloweach interval. The Y-axis in the lower panel shows the number of sequences scoring within the interval. Consequently, ... discussion The isochores in the chicken genomeIt should be noted that the chicken genome sequencestill contains a large number of gaps (Table 1). In the case of GGA1, there are 9847 gaps remaining. There-fore,...

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