Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... blast and tfasta analysis of the phCoADR revealed a signiﬁcant level of identityto putative NADH oxidases from hyperthermophiles and bacterial NADH oxidases from mesophilic sources(Fig. 1). Of ... correlates well with the catalytic constantobserved for the CoADR reaction.Thermostability and thermoactivity of the CoADRphCoADR is stable for months at both )80 °C and )20 °C, and has half-lives ... standards. Coenzyme A was measured as the sum of the areas of the CoA and dephospho CoA peaks, asdescribed previously [14].AcknowledgementsC. Davis, K. Dorschner and C. Hummel providedvaluable...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... in the Caryophyllaceae, Sambucaceae,Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and Poaceae [1]. Although many are potentially useful inagriculture and medicine because of their antiviralproperties ... displayed hemagglutination and toxicitytoward mice (data not shown), additional efforts tocleanly isolate the isoform were not successful and further characterization was abandoned. A chromato-focusing ... similarity of the A- and B-chains of the four isoforms, a pairwise alignmentwas performed and the values of identity expressed inFig. 3. (Continued). Characterization of four pulchellin isoforms...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The ampliﬁed PCR product wasinserted into the ... as an indicator substrate was used. The trans-formants were streaked on the plates and grown for 7 days, and tyrosinase activity was observed on the plates as a brown color appearing around the ... investigated fungaltyrosinases, from both a structural and a functionalpoint of view, are from Agaricus bisporus [10] and N. crassa [1]. Also, a few bacterial tyrosinases havebeen reported, of...

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... (degenerate reverse)55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward)65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR reverse)75¢-CGAGCTCACTGCCTCTCAAC-3¢ PsCBL (5¢UTR forward)85¢-ACTCGTAGC-ACAGAGACAGAG-3¢ ... forward)25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse)35¢-CTTAT(C ⁄ G)AACAAGGAA (A ⁄ C)AATTTC-3¢ PsCBL (degenerate forward)45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ ... merged images of A and B, D and E, I and J, and L and M, respect-ively.DiscussionNature has developed many pathways for combating and tolerating the the various stress signals that cross-talk...

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... follows: HSF 1a forward,5¢-GAAGCAGCTTGTCCAGTACACCAA-3¢; HSF 1a reverse, 5¢-TTCCAAGAGCTGAACAAACCATTG-3¢;HSF1b forward, 5¢-GAAGCAGCTGGTCCAGTACACCTC-3¢; HSF1b reverse, 5¢-GGCTGAATAAACCATGCCAGTAGC-3¢; ... Plasmid Cloningkit (Invitrogen) and was used as the PCR template. For 5¢-RACE, the first PCR was performed with the M13 reverseprimer (5¢-AGCGGATAACAATTTCACACAGG-3¢)asasense primer and a rainbow ... HSF 1a, 5¢-TGCCTTTTATGTTCTGCACGA-3¢;forHSF1b,5¢-CCTCCCTCCACAGAGCTTCA-3¢) as antisense primers. For 3¢-RACE, the first PCR was performed with the M13forward primer (5¢-CCCAGTCACGACGTTGTAAAACG-3¢)asasenseprimerandHSF1-specific...

Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

... Gly-Gln, Ala-Ala, Ala-Ala-Ala, Lys-Lys, d-aminolevulinic acid, cefadroxil,Gly, Pro-Ala, 8-aminooctanoic acid and Gly-Sar were from Sigma-Aldrich (Deisenhofen, Germany). Tert. butyloxycar-bonyl ... was subtracted from the total uptake to calculate the carrier-mediated uptake. Inset:Eadie–Hofstee transformation of the speciﬁc Gly-Sar uptake data[S, Gly-Sar concentration (mM); v, uptake ... h. The amount of Bip-Pro in the extracellular uptake mediumwas quantiﬁed according to the laboratory standard HPLC(La-ChromÒ; Merck-Hitachi, Darmstadt, Germany) with a diode array detector and...

Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx

... However, the backbone amide and carbonyl of Cys140 form hydro-gen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity and the replacement ... than Q14 3A hMnSOD mutants in the paraquat-containing media were selected for fur-ther characterization, and other mutants were discar-ded. Plasmids from the mutants that passed thissecondary ... wereplated again on 1 lm paraquat agar plates, so as toobtain approximately 500 colonies per plate. Afterincubation of these replated mutant colonies at 37 °C for another 20–24 h, the average...

Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... and XhoIML3p A- chain5¢-GATATACATATGTACCGTCGTATTAGCCTTCGTGTCACGGAT-3¢5¢-CACACGAATTCTTATTAAGAAGAAGAAGAACGGTCCCTGCATAC-3¢NdeI and EcoRIML3.1p A- chain5¢-GATATACATATGTACGAGCGTCTTCGTCTTCGTGTTACGCATC-3¢5¢-CACACGAATTCTTATTAAGAAGAAGAAGAACGGTCCCTGCATAC-3¢NdeI ... and XhoIML2p A- chain5¢-GATATACATATGTACGAGCGTCTTCGTCTTCGTGTTACGCATC-3¢5¢-CACACCTCGAGTTATTAAGAAGAAGACGGACGGTCCCGGCATAC-3¢NdeI and XhoIML3p A- chain5¢-GATATACATATGTACCGTCGTATTAGCCTTCGTGTCACGGAT-3¢5¢-CACACGAATTCTTATTAAGAAGAAGAAGAACGGTCCCTGCATAC-3¢NdeI ... containing the XbaI recognition site for cloning,5¢-AAAAATGCATGAAGTTGATTGCTTGCATTAACTCAT-3¢ (3¢UTR), 5¢-AAAAATGCATAGGGATGAAGTTGATTGCTTGCC-3¢ (3¢UTR) containing the NsiIrecognition site for...

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... His290 and Asp260 e merge from the b8 -a8 andb7 -a7 loops, respectively. Anotherimportant feature of the catalytic machinery is the so-calledoxyanion hole necessary for the stabilization of the oxyan-ion ... 5¢-gcgccaatcctggacgctgacgtcatcgacgcg-3¢ (forward) and 5¢-cgcgtcgatgacgtcagcgtccaggattggcgc-3¢ (reverse). The bases in bold indicate the location of the mutation. DNAsequence of the mutant was conﬁrmed ... strands associated to an anti-parallel strand (b2) and is surrounded by 5 helices (a1 , a2 , a3 , a7 anda8). T he second domain c onsists of helices a4 , a5 and a6 a ll clustered on the top of t he...

Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of speciﬁc proteins and functions docx

... identiﬁcation [5] of two morphologically distinctclasses of caveolae at the plasma membrane ) canon-ical caveolae that are open to the extracellular space and caveolae lacking access from the cell ... labelling that wasagain limited to the HD-caveolae and LD-caveolae and especially the LD-caveolae (not shown). Toexamine whether the biotin labelling was restricted to the LD-caveolae, we separately ... HD-caveolae (lanes b1 and b2); LD-caveolae (lane c). a, b1 and b2, and c were separately subjected to SDS ⁄ PAGE (9% acryla-mide) and silver staining. Indicated are apparent molecular massesof...

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