Báo cáo khoa học: Identification of versican as an isolectin B4-binding glycoprotein from mammalian spinal cord tissue pptx
Báo cáo khoa học: Identification of versican as an isolectin B4-binding glycoprotein from mammalian spinal cord tissue pptx
... Oxford.IB4-binding versican in spinal cord tissue O. Bogen et al.1102 FEBS Journal 272 (2005) 1090–1102 ª 2005 FEBS Identification of versican as an isolectin B4-binding glycoprotein from mammalian spinal ... unam-biguously as a V2-like variant of versican. Moreover, we demonstrate thatthe IB4-reactive glycoconjugate and the versican variant can be co-released from spinal cord membranes by hyaluronidase, and ... peptide mass fingerprint. Versican and IB4-binding activity are co-enrichedby subcellular fractionation of spinal cord tissue It is known that the association of versican with theplasma membrane...
- 13
- 447
- 0
Báo cáo khoa học: Identification of NF1 as a silencer protein of the human adenine nucleotide translocase-2 gene pptx
... gene and 2 lg of controlluciferase plasmid DNA (pGL3, Promega) were used fortransfection. CAT and luciferase activities were measured as described [17].DNase I protection assayRat liver and ... nucleotide translocase and glycerol 3-phosphateFig. 6. Site-2 and Site-3 elements act in a concerted manner to cause therepression of ANT2 promoter. Transient transfections analysis of theheterologous ... immunoprecipitated with no antibody (lanes 2 and 7), or withanti-NF1 8199 (lanes 4 and 9), or with unrelated antibodies (lanes 3and 8). After reversal of the crosslink, DNA was amplified by PCRusing...
- 8
- 426
- 0
Báo cáo khoa học: Identification of calreticulin as a ligand of GABARAP by phage display screening of a peptide library pdf
... 9 and 18 lmand koff> 1000 s)1, much weaker than that withGABARAP [37]. Glycans bind CRT with dissociationconstants as low as 435 nm and koff¼ 0.1 s)1[38].This is still significantly ... primary antibodieswere mixed and applied simultaneously. The concentration of the antibodies, laser intensity and filter settings werecarefully controlled, and the sequential scanning mode wasemployed ... kofftobe 8.3 · 10)4s)1. Rmaxvalues decreased, as expected,with increasing baseline. A dissociation constant of 64 nm for the GABARAP–CRT interaction wasobtained. The overall fit of...
- 13
- 560
- 0
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... EMSA was performedwith RGG3 (lanes 2 and 4) and32P-labeled Htelo (lanes 1 and 2) orrHtelo (lanes 3 and 4). (B) EMSA was performed with RGG3 (lanes2 and 4) and32P-labeled rHtelo (lanes 1 and ... with EWS (lanes 2, 4 and 6) and32P-labeled Htelo (lanes 3and 4), dsHtelo (lanes 5 and 6) or ssDNA S (lanes 1 and 2). Thestructures of DNAs used as probes are indicated above each lane.The ... activities of EAD (lane2), RGG1 (lane 3), RRM–RGG2–ZnF (lane 4)and RGG3 (lane 5). EMSA was performedwith these proteins and32P-labeled Htelo.(C) EMSA was performed with RGG3 (lanes2 and 4) and32P-labeled...
- 11
- 786
- 0
Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
... templates and primers: total RNA from O35E (lanes 1 and 3),O35ElpxX (lanes 4 and 6) and O35ElpxL (lanes 7 and 9), and chro-mosomal DNA from O35E (lane 2), O35ElpxX (lane 5) and O35El-pxL (lane 8). ... acyltransferase genes encoding decanoyltransferase and dodecanoyl transferase from M. catar-rhalis serotype A strain O35E, and constructed thecorresponding isogenic mutants. Analysis of physio-chemical ... physio-chemical and biological features of both mutants wasperformed to study the functions of these genes andthe structures of their resultant LOSs in vitro andin vivo.Results Identification of putative...
- 14
- 674
- 0
Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx
... hybridizationMessenger RNA was extracted from mantle and gills of oysters exposed to 13 or 25 °C after 7–10 and 24 days.Two micrograms of mRNA (1 lg from the gills and 1 lg from the mantle) were used as the template ... 6393D9-desaturase and glutamine synthetase) showed apeak of overexpression at days 14 and ⁄ or 17. In con-trast to observations on gill tissue, mantle levels of HSP12A, MTA-1 protein, Rho1p, D9-desaturase ... detoxification, membrane fluidity,energetic metabolism and translational processing. TotalRNA was extracted from gills and mantle of 10 oystersexposed to 13 and 25 °C for 0, 3, 7, 10, 14, 17 and 24 days.A...
- 11
- 570
- 0
Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
... substances may be aggregates of proteins and polysaccharides, and may play importantroles in formation of the phases and ⁄ or morphologies of the crystals because they consist of acidic glycoproteinsand ... macromolecule-64 (OMM-64), was identified. Theprotein was found to have a molecular mass of 64 kDa, and to containtwo tandem repeats and a Glu-rich region. The structure of the proteinand that of its DNA are ... School of Agricultural and Life Sciences, University of Tokyo, Japan2 Division of Marine Biosciences, Graduate School of Fisheries Science, Hokkaido University, JapanOrganisms can design and shape...
- 12
- 568
- 0
Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
... suspen-ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed for 2 h. Products were extractedwith 50 mL of hexane. The hexane layer was washed with25 mL of saturated sodium ... first identification of triterpene hydroxylase cDNA from any plantspecies. Successful identification of a b-amyrin and sophoradiol 24-hydroxy-lase from the inducible family of cytochrome P450 ... extracts of these trans-formants with14C-labeled b-amyrin as a substrate didnot show any hydroxylase activity, and in vivo assayby feeding the same substrate to the culture of eachtransformant...
- 12
- 704
- 0
Tài liệu Báo cáo khoa học: Identification of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc
... play an important role inmediating the transcription of these genes.The c-fos gene was examined in this system as an example of a GAS-driven gene whose IFN-inducibilityis independent of both ... [3,4].Arguably the most notable of these cascades is theJanus kinase (Jak)-signal transducer and activator of transcription (STAT) pathway that regulates thetranscription of numerous IFN-sensitive genes ... PKR, 2¢5¢OAS and Mx1;genes whose products are known mediators of theIFN-inducible antiviral response [16]. Comparabletranscriptional activation of the PKR, 2¢5¢OAS and Mxgenes was observed...
- 13
- 459
- 0
Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
... milk and successively treated with an anti-TAF ⁄ IGFBP-rP1 monoclonal antibody (#88) or an antimatriptase monoclonal antibody (M32) as the first anti-body, the second antibody (biotinylated antimouse ... from humanbladder carcinoma cells [4,5]. Its cDNA was cloned from human leptomeningial cells as mac25 [6] and from human fibroblasts as prostacyclin-stimulating factor(PSF) [7]. Since AGM has ... Yana I (2003) Roles of pericellular proteolysisby membrane type-1 matrix metalloproteinase in cancerinvasion and angiogenesis. Cancer Sci 94, 569–574.19 Blasi F & Verde P (1990) Urokinase-dependent...
- 13
- 603
- 0
Từ khóa: chuyên đề điện xoay chiều theo dạngNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Chuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIMÔN TRUYỀN THÔNG MARKETING TÍCH HỢP