Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot

... 685–695 ª 2005 FEBS 695 Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli Rose-Marie Knutsson Jenvert1,2 and Lovisa Holmberg ... the P-sites of the 30S and 50S subunits and the tRNA Phebound in the A-site of the 30S and 50S sub-unit [28]. Moreover, a tRNA Metfwas present in the 50SE-site. The E-site bound tRNA did not ... required that the A-site was saturatedwith unacylated tRNA. Is the tRNA- stimulatory effect affected by the order of addition of tRNA and SF to the reactions? The two types of tRNA titration...

Báo cáo khoa học: Characterization of the lipopolysaccharide and b-glucan of the ﬁsh pathogen Francisella victoria ppt

... analy-sis of the LPS from related species is important inorder to gain an understanding of the molecular basis of their biological properties, the host specificity and diversity of members of this ... mannose residues. The b-configuration of the Man F followed from the observa-tion of intraresidual strong NOEs between H-1 and H-3, H-5, and also from the low field position of the C-5 signal at ... of AA at 170.9 p.p.m. and H-4 of the Qui4N, indicating acylation of NH2-4 of Qui4N withone of AA carboxyl groups. Spectra contained signals of one N-acetyl group acylating N-4 of the AA. The ESI...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... FEBS([1] and references therein). The auto-inhibitory action of the C-terminus is suppressed, besides by pH, by lysophospholipids and by 14-3-3 proteins ([1] and ref-erences therein). The latter ... of the ﬁrst 88amino acids of PPI1 may participate in the interactionwith the H+-ATPase and makes PPI1588His6a suitabletool to study the mechanism of action of PPI1. The analysis of the ... enhances the activity of the enzyme whoseC-terminus has been displaced by low pH or by FC-induced binding of 14-3-3s.Results The C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1)...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... SNO2,SNO3, SNZ2 and SNZ3 complement the defect. The relationship of all of these genes remains to be clarified.Expression and purification of Sno1p and Snz1pIn light of the similarity in the amino ... hydrolysis mediated by the complex of Sno1p with a His-tag and Snz1p. The reaction wascarried out in 1 mL of 50 mMTris/HCl, pH 8.0, in the presence of 1–10 mMglutamine and 50 lg of the complex, ... min, the absorbance of the supernatant was read at 363 nm. The absorbance of the reduced form of APAD (APADH) was linear over the 2–100 lMrange of glutamate with a molar extinctioncoefficient of...

Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc

... transcription factor of the Ets-family is involved in the regulation. The members of the Ets-family of transcription factors bind to speciﬁcpurine-rich sequences with a core motif of GGAA/T and control the ... CCAAT box at 324 bp upstream of the major transcription start site. The presence of a CCAATbox and the absence of a TATA box seem to be one of the characteristics of the mouse integrin a3 gene.We ... reported the structures of the mouse a3integrinsubunit gene including the exon/intron organization and the alternative exon usage for the generation of variants of the a3 subunits (a3A and a3B)...

Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt

... Cleavage of the C9–C10double bond (a) results in products 1 and 2 from I, products 1 and 3 from II, and products 3 and 4 from III. The cleavage of (b) leadsto product 4 from II, and product 6 from ... that the preference of OsCCD1 for the C5¢–C6¢ and C7¢–C8¢ double bonds depends on the nature of the substrate. The cleavage of a sole double bond in the cyclic moi-ety of 3-OH-c-carotene and of ... dialdehydes of different chain lengths (1, 4 and 5 from II; 4, 6 and 7 from III), asindicated by their UV ⁄ visible-spectra (shown in the insets) and retention times. (B) Structures of the synthetic...

Báo cáo khoa học: Characterization of the NADH:ubiquinone oxidoreductase (complex I) in the trypanosomatid Phytomonas serpens (Kinetoplastida) ppt

... workwas supported by grants from the Grant Agency of the Ministry of Education of the Slovak Republic and the Slovak Academy of Sciences 1 ⁄ 3241 ⁄ 06 (toA. Horva´th), and the Comenius University ... excluded the possibility that the  2.2 MDa large complex is just an oligomer of the small form. The different inﬂuences of DPI on the two forms could be caused by its failure to access the ﬂavin cofactor ... absent from the lower band (Fig. 2; arrows).Owing to the horizontal smear, however, the presence of the B14 subunit in the lower band cannot be ascer-tained at this point. A cross-reaction of the...

Báo cáo khoa học: Characterization of the bioactive conformation of the C-terminal tripeptide Gly-Leu-Met-NH2 of substance P using [3-prolinoleucine10]SP analogues pdf

... dramatic loss of affinity and potency. To analyze the structural implications of thesebiological results, the conformational preferences of the SP analogues were analyzed by NMR spectroscopy and minimum-energy ... removal of the last Na-Boc-protecting group, the resinwas dried in vacuo. The peptide resin was transferred into the Teflon vessel of an HF apparatus and the peptide wascleaved from the resin by ... methanol [35]. The random coil value of Pro was used for the different prolinoamino acids. Therefore, the CSDs of P3Leu10 depend primarily on the chemical modification of the pyrrolidine ring and cannot...

Báo cáo khoa học: Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli pptx

... forms: 38 and 40 kDa (Fig. 5A). The proﬁle of recombinant GluV8 was essentially identical to that of native GluV8, except for the presence of the non-degraded 41–44 kDa bands of the recombinant ... itwas concluded that the full-length form of GluV8could be recovered quantitatively by the suppression of self-degradation, either by the use of the GluSEprepropeptide or the GluV8 prepropeptide ... processed by thermoly-sin, its overall structure does not appear to be altered from the active form. Therefore, to elucidate the mech-anism of suppression of the protease activity and the alteration...

Báo cáo khoa học: Characterization of the bga1-encoded glycoside hydrolase family 35 b-galactosidase of Hypocrea jecorina with galacto-b-D-galactanase activity pdf

... of buffer A. The columnwas then washed with further 10 column volumes of buf-fer A, and thereafter the bound proteins were eluted by applying a linear gradient of 0–0.6 mm NaCl in a total of 40 ... All these positions are located at the surface of the jelly roll domain of Bga1 and thus most probablyare glycosylated in vivo. To further substantiate theseﬁndings, in gel digestion of the ... Establishment of the exact molecular mass by MS and pro-tein purity by capillary gel electrophoresis-on -the- chip. (A) Determin-ation of the exact molecular mass of H. jecorina Bga1 by positiveion...

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