... protein
sequencer.
Results
Purification of aspartate racemase from
B. bifidum
The aspartate racemase was purified to homogeneity from
cell extracts of B. bifidum NBRC 14252. A summary of the
purification is presented ... Linear alignment of amino acid se-
quences of PLP-independent aspartate race-
mases. Bb, the monomeric aspartate racemase
from B. bifidum; St,...
... (nm) of heme absorbance of the oxi-
dized forms of GST-fused IZAs.
Table S2. Peaks (nm) of heme absorbance of the
reduced forms of GST-fused IZAs.
Table S3. Results of GST pull-down assay of the
incubation ... mixture of GST-rIZA1 and [
3
H]progester-
one.
L. Min et al. Molecular properties of adrenal inner zone antigen
FEBS Journal 272 (2005) 5832–5843 ª 2005 FEBS 5843...
... libraries were made on 2 lgof
mRNA extracted from the 10 oysters collected after
30 days of exposure and pooled for RNA extraction.
mRNA from the gill and the digestive gland (1 lg from
each) were used ... coastal waters by runoff. Moreover, the exposure
of animals in these ecosystems to pesticides is never
limited to a single pollutant, but to an assortment of
chemicals from a...
... MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H. jecorina grown on malt
agar. The masses displayed have an error of about 0.1%, so peaks of 7232 ... number of small samples, we have
investigated the potential of intact-cell MALDI-TOF
MS (ICMS). Initial applications of ICMS on fila-
mentous fungi have demonstrated characteristic set...
... large volumes of data can
be used to gather world knowledge for natural lan-
guage processing. A consequence of this research
was the creation of
It-Bank
, a collection of thou-
sands of labelled ... describes our data for learning
and evaluation,
It-Bank
: a set of over three thousand
labelled instances of the pronoun it from a variety
of text sources. Section 4 also explai...
... molecular
masss are indicated in lane 1. Lane 2, material from the acid extracts
of granules; lane 3, material from fractions 58–69 of the Sepha-
dex G-75 purification step; lane 4, material from ... FEBS
Discussion
This study has shown the identification, purification
and characterization of a novel protein from acid
extracts of granules of neutrophil granulocytes of
heal...
... resolved the
molecular masses, and yielded an observed mass of
Fig. 2. Isolation of internal peptides from PSRP-2 and PSRP-3. (A)
HPLC separation of endoproteinase Lys-C Ôin-gelÕ digest of PSRP-2
(10 ... extracted from 2D gel spots). Peptide 2 and the N-terminal
peptide were sequenced. CBB, peak of Coomassie Blue. (B) HPLC
separation of endoproteinase Asp-N digest of PSRP-...
... simi-
lar to that of the mixture of thermal stabilization
products of the [5]-ladderane fatty acid 7a apart from
the fact that the m ⁄ z values of the molecular ion and
some of the characteristic ... iso-
thermal part of the temperature program) of
fraction CC5 (fraction 5 obtained by prepara-
tive column chromatography of the large
batch of cell material) of the extra...
... al., 2008).
Two of the features used in (Liu et al., 2006)
in identification of the navigational queries from
click-through data, are the number of Clicks Satis-
fied (nCS) and number of Results Satisfied ... classifier
outperforms some of the best methods that
have been proposed for the task of written
language identification on the domain of
search engine queries.
1 Introduct...
... addressed by bootstrapping from a
relatively small set of hand-authored utterances that
perform key dialogue acts in the scenario or from
utterances collected from wizard -of- oz or role-play
exercises, ... utterance result in sparse
coverage of the space of possibilities, unless a very
large amount of data can be collected and anno-
tated, which is often impractical. Users of...