... mAb didnot show any cross-reactivity to other proteins, suchas human serum albumin (HSA), BSA, human IgA, human IgM, human IgE, human hemoglobin, KLHand tumor necrosis factor-b (TNF-b)(Fig. ... [13].Recombinant human hTNF-a was graduallydegraded by ETNF-6-H during 94 h of incubation.The cleavage sites, which were confirmed by N-termi-nal amino acid sequencing, are indicated by red arrowsin ... mAb with irrelevantproteins by ELISA. ETNF-6 mAb showed high specificity for hTNF-a.The mAb did not react at all with human TNF-b at all.Catalytic digestion of TNF-a by the antibody subunit E....