... possible linke r
between type VI and type II collagen [24]. In initial
experiments we studied the interaction of the various
decorinformswithtypeVIcollageninasolidphase
binding assay. Decorin ... collagens and decorin are
of paramount importance in attaining and maintaining
tissue integrity.
In the present study we investigate the binding properties
of wild -type dec...
... with Mxi1-SRa and enhances its ability to
activate the Myc promoter. Our findings suggest that Mxi1 gains
functional complexity by encoding isoforms with shared and distinct
activities.
Abbreviations
FITC, ... (Fig. 4B).
The finding of these differential functions is in line
with the dogma that the proteome gains functional
complexity by encoding multiple isoforms of a given
pro...
... This indicates a higher
degree of freedom of the native enzyme, i. e. higher
structural flexibility, which is reflected in higher specific
activity [3]. The calorimetric enthalpy of denaturation
of ... pattern of proportional pooling of subunits
with different kinetic and thermodynamic properties of the tetrameric enzyme
may result in fine-tuning of the properties of skelet...
... electrophoretic mobility shift assays, the binding
reaction was carried out in 16 lL of mixture containing
0.2–2.0 lLofin vitro transcription ⁄ translation reaction
mixture (0.9–5.8 ng of hHSF polypeptides), ... expres-
sion of different hHSF target genes is dependent upon
the architecture of the HSE.
Results
Human HSF1, HSF2 and HSF4 exhibit differential
binding specificities for...
... glucocorticoid inhibition, suggesting that
inhibition of COX-2 is critical in t he repression of
prostaglandins by glucocorticoids. As cytokine-induced
COX-2 and PGE
2
release are highly NF-jB-dependent ... including increased
gluconeogenesis, i ncreased blood glucose, amino and fatty
acid mobilization, and loss of bone [2]. In addition,
endogenous glucocorticoids p articipate i...
... regulation of cyclin A and B1 upon TSA treatment is
via diminished activity of NF-Y-associated HAT and
is mediated through CCAAT motifs; further, the
diminished HAT activity is mediated by ... capability of degrading
the majority of ECM proteins [2]. Four tissue inhibi-
tors of metalloproteinases (TIMPs) safeguard ECM
integrity by virtue of their ability to inhibit the
MMPs [3...
... sialidase activity against mixed
gangliosides (MG, Fig. 2), suggesting that the siali-
dase activity measured with mixed bovine gangliosides
was not due to the activity of Neu1. Thus, the activ-
ity ... endogenous sialidase
activity of monocytes increases as they differentiate
in vitro into macrophages.
The increase in activity of lysosomal sialidase
Neu1 during monocyte differentiati...
... Department of Proteomics and Signal Transduction, Klopferspitz, Martinsried, Germany
2 Hospital Universitario Canarias, Center Biomedical Research on Rare Diseases (CIBERER) and Institute of Biomedical ... converting it into glycine. The
enzyme is present in peroxisomes and ⁄ or mitochondria
in different mammalian species, with peroxisomal AGT
being mainly responsible for the deto...
... D’Alessio
1
and
Claudia De Lorenzo
1
1 Dipartimento di Biologia Strutturale e Funzionale, Universita
`
di Napoli Federico II, Italy
2 Dipartimento di Chimica, Universita
`
di Napoli Federico II, Italy
ErbB2 ... a single set of identical independent bind-
ing sites, using the Bindwork software supplied with the
instrument, which provided the stoichiometry of binding (n
ligand : protein...
... subunit has three binding sites for the divalent
cation, namely a conformational site (site I) , a catalytic
site (site II) and an inhibitory site (site III) [39–42]. As
binding of divalent cation ... enolase: visualisation of the inhibitory metal
binding site III and potential as target for selective,
irreversible inhibition. J Mol Biol 331, 653–665.
42 Lee BH & Nowak T (1992) I...