Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding Cyril Barˇinka1, Petra ... residues and other amino acids situated in the vicinity of these residues. The resultsconfirmed the importance of the amino acid residues, alllocated at the putative catalytic domain, for the GCPIIhydrolytic ... analyzed the contribution of the N- and C-terminal regions of GCPII to its enzymatic properties and structure /folding. The results clearly show that the amino acids at the extreme C-terminus of GCPII...
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Báo cáo khoa học: UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity potx
... the strain 168 [22] as the template and with the following primers: 5¢-pyrH,5¢-GGGGCATATGGAAAAACCAAAATACAAACGTATCGTATTA-3¢ ;and 3¢-pyrH,5¢-CCCCCTCGAGTTATTTCCCCCTCACGATCGTTCCGATTGATTCAC-3¢. The ... as the ATP-binding site, while the N-terminaldomain would contain the cosubstrate and the effectorbinding sites. The dimerization interface is composed of a-helices and a b-strand from the N-terminal ... GTP, the rates were maximal at 50–70 lMUMP (Km for UMP 8 lM) and declined upon furtherincreases in UMP. In the presence of GTP, saturation wasattained at 0.2 mMUMP and the apparent...
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Tài liệu Báo cáo khoa học: Dmrt1 genes at the crossroads: a widespread and central class of sexual development factors in fish pdf
... all the evidence suggests that sex determinationmight disobey the conventional rules of evolutionaryconservation. The common picture emerging here is that the genes at the top of the cascade are ... or the germ cells indicated that mouse Dmrt1 is indeed required for radial migration of germ cells and survival of gonocytes. It is also required autonomously for proper Sertoli cell differentiation ... compilation ª 2011 FEBSscheme that, whatever the sex-determination system,more comparative studies of dmrt1 are required inorder to draw the first lines of a global core regulatorynetwork for...
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Tài liệu Báo cáo khoa học: Gene silencing at the nuclear periphery pdf
... epigeneticmodifications. The two chromatin states, of gene-active unwrapped euchromatin at the nucleoplasm, and of gene-silenced condensedheterochromatin at the vicinity of the INM are circled. In the latter ... degeneration of the caudate nucleus, putamen, and occasionally the globus pallidus, with little involve-ment of the rest of the brain. The question of how mutations in the same gene orgroup of ... For example, the combination of H4 K8 acetylation, H3K14 acetylation, and H3 S10 phosphorylation is oftenassociated with transcriptional activation. Conversely,tri-methylation of H3 K9 and the lack of...
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Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx
... function of wild-type eEF2 thereby reducing the rate of proteinsynthesis and the growth rate of the transformed cells.As shown in Fig. 3B, the doubling rate was only mar-ginally affected by the ... (C). The cells were allowed to grow at 30 °C under vigorous shaking. The attenuance of the yeast cultures wasmeasured at 600 nm at the intervals indicated (A–C) and the growth rates calculated ... caused by the amino acid replacement at position 56 because the effect on the growth rate wassimilar to that observed with the single mutationT56M. The data suggest that phosphorylation at posi-tion...
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Báo cáo khoa học: Transcription termination at the mouse mitochondrial H-strand promoter distal site requires an A/T rich sequence motif and sequence specific DNA binding proteins pptx
... region of the genome. The D loop is created by the displacement of one of the parental strands by 0.5–1 kbnascent DNA strand needed for the replication of the Lstrand. The 0.8–1 kb long D loop ... transcription termin-ation at the end of the 16 S rRNA gene. The putativetridecamer terminator sequence has been mapped to the 5¢end of the tRNALeugene, which occurs immediatelydownstream of the 16 ... populations of H-strand transcripts escapetermination at the mt-TERM and continue through the distal sites of the genome.Studies from two laboratories showed that the D-loopregions from the rat...
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Báo cáo khoa học: Proteome analysis at the level of subcellular structures Mathias Dreger pot
... appar-atus, as compared to the relatively straightforward prepar-ation of nuclei, is that the procedure comprises a series of density centrifugation steps as the physical properties of the material ... Despite the exceptional analytical power of this approach, system-atic limitations of the approach at the present state of the technology became apparent. There are certain classes of proteins, ... al.[27]reported upregulation of a number of Golgi proteins inGolgi preparations from rat mammary gland cells in the state of maximal secretion at lactation as compared to thatin a state of basal secretion....
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Tài liệu Báo cáo khoa học: Poly(ADP-ribose) The most elaborate metabolite of NAD+ Alexander Burkle pptx
... groups.Attractive scenarios developed over the last couple of years are (a) the localized relaxation of chromatin at the site of DNA damage, mediated either by direct modifi-cation of histones ... tobe relevant for the inhibitory effect of PARP-10 con-cerning c-Myc- and E1A-mediated cotransformation of rat embryo fibroblasts.Conclusion and outlook The field of poly(ADP-ribosyl)ation is currently ... between PARP-1 and PARP-2, including size and the absence of zinc fingersor the BRCT motif, they are both targeted to the nuc-leus, and they bind to and become activated by DNaseI-treated DNA. Both...
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Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf
... 4A). The rapid forma-tion of the charge-transfer intermediate preventedaccurate analysis of the rate constant for its formationby the stopped-flow method; the rate constant for col-lapse of the ... inhibitor rather than a substrate for this enzyme. The presence of Cb atom at posi-tion 26 therefore causes the substrate specificity of Table 2. Kinetic parameters for the reductive and oxidative ... function of NADPH concentration for H181A (filled squares) and H184A (filled circles). (B)dependence of the observed rate for the oxidation of reduced FMN on 2-cyclohexen-1-one concentration for the...
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Báo cáo khoa học: Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding pdf
... showing thatdeletion of the N-domain of ClpA dramaticallyreduced the rate of degradation of GFP–ssrA and to alesser extent casein [8,11], we speculated that the N-domain facilitates an early ... that the defectin RR ⁄ AA-mediated unfolding is not dependent on the global thermodynamic stability of the substrate,but rather correlates with local unfolding of the sub-strate (i.e. unfolding ... using mutational analysis, we report the iden-tification of a structural element composed of con-served basic amino acids (R90 and R131), locatedwithin the N-domain of ClpA, that dramatically...
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