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Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf

Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf

Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf

... NMR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of ... establish the preferred geometry of the precursor. We now report the chemical synthesis, bio-chemical production, and solution structure of preCbnB2, and compare it with the structure of the mature bacteriocin, ... determine the structural basis of the inhibition of the antimicrobial activity of CbnB2 by the leader and to assist future analysis of the interaction of preCbnB2 with its ABC transporter protease, it...
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Báo cáo khoa học: Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase pdf

Báo cáo khoa học: Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase pdf

... a continu-ous strand [1]. During DNA replication, a helicase sep-arates the double-stranded DNA into single strands, and replication of the leading strand and synthesis of the Okazaki fragments ... Stereo views of the solution and crystal structures of DnaG-C. (A) Superposi-tion of the backbone atoms of residues447–581 of the 20 NMR conformers of DnaG-C representing the NMR structure (Table ... binds to the core of the other in a manner similar to that in whichhelix 6 binds to the core of the structure in the mono-meric solution structure (Figs 3A and 4). The two conformers in the crystal...
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Báo cáo khoa học: NMR solution structure of Cn12, a novel peptide from the Mexican scorpion Centruroides noxius with a typical b-toxin sequence but with a-like physiological activity doc

Báo cáo khoa học: NMR solution structure of Cn12, a novel peptide from the Mexican scorpion Centruroides noxius with a typical b-toxin sequence but with a-like physiological activity doc

... of discriminating betweenNa+-channel isoforms of the same organism (e.g. the ratbrain isoform rNaV1.1 is 10-fold more sensitive to the action of the a Na-ScTx Lqq5 than the cardiac isoform ... knew that the remaining disulfidebridges were between Cys25 and Cys45 and Cys29 and Cys47, or conversely between Cys25 and Cys47 and Cys29 and Cys45. The sequences of the fragments from the chymotryptic ... peptides (23–41-amino-acidslong) which recognize and bind to various types and subtypes of K+channels [13,19], ryanodine-sensitive Ca2+channels [11], and Cl–channels [30]; long-chain peptides(59–76...
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Báo cáo khoa học: Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion pot

Báo cáo khoa học: Atomic-resolution structure of reduced cyanobacterial cytochrome c6 with an unusual sequence insertion pot

... inhibitor structure (3.44–3.58 A˚). The stability of Tyr56 may be important for the conformation of Gln57, and consequently for tuning the redox potential of the protein. Phe68 forms part of a conserved ... because of its very high content (36%) of the smallest resi-dues (glycine and alanine). The structure reveals that the overall fold of the protein is similar to that of other class I c -type cytochromes, ... inside the heme pocket forms a hydro-gen bond with the Gln57 side chain, and is thusresponsible for positioning of the c-amide towards the heme group and within a van der Waals distance of amethine...
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Báo cáo khoa học: Change in structure of the N-terminal region of transthyretin produces change in affinity of transthyretin to T4 and T3 pdf

Báo cáo khoa học: Change in structure of the N-terminal region of transthyretin produces change in affinity of transthyretin to T4 and T3 pdf

... demonstration of the rela-tionship between the evolution of the primary structure of the N-terminal regions of TTR and the biologicalfunction of TTR.ResultsSynthesis of recombinant human TTRIn the crocodile, ... 2006)doi:10.1111/j.1742-4658.2006.05404.x The relationship between the structure of the N-terminal sequence of trans-thyretin (TTR) and the binding of thyroid hormone was studied. A recom-binant human TTR and two derivatives of ... whetherthere is a causal relationship between these two types of changes. The work reported here is a planned quantita-tive analysis in vitro of the relationship between the twotypes of changes.In...
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Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf

Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf

... alignment of eRF1.Fig. S11. The Ramachandran map plot (/ and w tor-sion angles for the protein backbone) of all 24 con-formers of the NMR families of solution structures of the closed and open conformers ... conformer and near Glu365 and Glu367 in the closed conformer, and these polarinteractions may play an important role in the stabil-ization of the two conformers. The two His residues,His334 and ... important for the determination of the structures of the conformers of the C-domain. The dipolar couplings provided long-distance informa-tion on the global folding of both conformers. The structure...
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Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx

Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx

... c. The Soret absorbance band is red-shifted (more pro-nounced in the case of the K13N mutant), and the absorbances at 497 and 528 nm, in the visible region,are inverted, whereas the CT band ... influenced by the type of anion in solution [17,18].Because structural information on partially foldedforms is important for a deeper understanding of the folding mechanism(s) and the factors ... and LS stand for high- and low-spin, and IHH and IHMindicate the bis-histidine and the His-Metcoordinated intermediates) we observe that the sulfateinduces a similar kinetic pathway to the...
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Báo cáo khoa học: Hexameric ring structure of a thermophilic archaeon NADH oxidase that produces predominantly H2O pot

Báo cáo khoa học: Hexameric ring structure of a thermophilic archaeon NADH oxidase that produces predominantly H2O pot

... conditions asused for the wild -type. The results showed that the C122A mutant had similar NADH and NADPH oxi-dase activity to that of the wild -type protein; however, the C45A mutant and the double ... NADH and NADPHas electron donors. In addition, the activity and prod-uct assays of the wild -type and mutants showed thatCys45 is the active site residue and that Cys122 doesnot function in the ... ATG-3¢; anti-sense primer, 5¢-TAN ACT TTY TCN CAN SWN GTYTGC AT-3¢; N = A, G, C and T; Y = C and T; W = A and T). The sense primer was designed from the knownN-terminal sequence, and the antisense...
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Báo cáo khoa học: X-ray structure of peptidyl-prolyl cis–trans isomerase A from Mycobacterium tuberculosis potx

Báo cáo khoa học: X-ray structure of peptidyl-prolyl cis–trans isomerase A from Mycobacterium tuberculosis potx

... byenzymes in the new group is also likely to resemble that of the human PpiA and PpiB and most others. In the bindingsite of the B. malayi Ppi,theequivalentofAla118isFig. 4. Sequence alignment. The ... target.Acknowledgements The authors thank Markus Dalin for help with cloning and i nitialcrystallization experiments, Andrea Wilnerz on and Jimmy Lindbe rg for help with cry stall ization, and Jenny Berglund and Annette ... kcat/Km of 2.0 · 106M)1Æs)1 ,and therefore shows s imilar activity to the Ppi from Brugiamalayi,withakcat/Km of 7.9 · 106M)1Æs)1[26], and tohuman PpiA, with a kcat/Km of 1.4...
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Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf

Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf

... to the physiologically relevantligand, (2R)-glyceryl-b-d-galactopyranoside, solved by molecular replace-ment, and refined to 1.87 A˚resolution with R and R-free values of 17% and 22%. The structure ... lactose, is the natural substrate of the system. Thus, GGal taken up by the Mgl trans-porter will induce expression of the lac operon, and sopromote further uptake and utilization of the com-pound. ... O2¢. These interactions increase the number of hydrogen bonds between the protein and ligand by five compared with the monosaccharides. The glyceryl moiety of GBP lies near the hinge of the protein,...
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