Báo cáo khóa học: A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum docx

... A new UV-B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum Avital Torres1, Malka Hochberg4, Inna Pergament1, Reem Smoum1, Valerie ... photo- synthetic organisms such as lichens, fungi, cyanobacteria,corals and other marine organisms are much moreadvanced than those of mammals because photosyntheticorganisms depend on solar irradiation ... (mycobiont) and lichen Collema cristatum. The mycobiont has a maximum at 311 nm.Fig. 3. Eﬀect of the pure compound isolated from Collema cristatum onkeratinocyte survival after UV-B exposure. HaCaT...

Tài liệu Báo cáo khoa học: A novel dicyclodextrinyl diselenide compound with glutathione peroxidase activity ppt

... CumOOH, and NADPHwere also obtained from Sigma. Sephadex G-25 was pur-chased from Pharmacia (Uppsala, Sweden). All the othermaterials were of analytical grade and obtained from Beijing Chemical ... such asinstability, antigenicity and poor availability, muchattention has been paid to its artiﬁcial imitation [9,10].In synthetic approaches, an initial attempt is madeto synthesize organoselenium ... has the same catalyticmechanism as native GPX. From the steady-state equa-tion, the kinetic parameters were obtained (Table 2).During investigation of the reduction of peroxides, itis natural...

Tài liệu Báo cáo khoa học: "A New Dataset and Method for Automatically Grading ESOL Texts" pdf

... that treating AA as a text classifica-tion problem is viable, but the feature types are allfairly shallow, and the approach doesn’t make effi-cient use of the training data as a separate classifieris ... complexity and deviation of a sentence from the parser’s grammatical model. This approachbears some similarities to our use of grammaticalcomplexity and extragrammaticality features, butgrammatical ... a head and dependent in a grammatical re-lation (GR) from the RASP GR output, calculatedfor each GR graph from the top 10 parses per sen-tence. In particular, we extract the mean and medianvalues...

Báo cáo khoa học: A new bright green-emitting ﬂuorescent protein – engineered monomeric and dimeric forms pptx

... the most appropriate for their speciﬁc research application. With regards to photostability, VFP and its variantsphotobleached at a faster rate than EGFP. The vastmajority of reports in the literature ... cocktail (Roche) and 5 mm b-mercapto-ethanol. The lysate was sonicated, then centrifuged. The supernatant solution was loaded into Ni-nitrilotriacetic acidagarose (Qiagen, Valencia, CA, USA), and ... that mature more rapidly at highertemperatures. Here we describe the characterizationand modiﬁcation of a novel FP that was isolated from Cyphastrea microphthalma, a scleractinian coral foundin...

Báo cáo khoa học: A new phospholipase A2 isolated from the sea anemone Urticina crassicornis – its primary structure and phylogenetic classiﬁcation pptx

... IPLA2s than UcPLA2(Fig. 3A) . Other known cnidarianPLA2s, such as those from the hydrozoan Hydra magni-papillata [34] and the sea anemones A. pallida [16,17]and B. caissarum [19], as ... huntingand defense) of the sea anemone Adamsia carciniopados(AcPLA2) [20]. Nested RT-PCR with degenerateprimers and RACE was used to clone the PLA2 from this animal. AcPLA2contains a putative ... Japan).RACE5¢-RACE and 3¢-RACE were performed on the RACE-ready cDNA prepared from previously isolated total RNA with the GeneRacer Kit (Invitrogen, Carlsbad, CA, USA).For the 3¢-RACE reaction,...

Báo cáo khoa học: A new approach for distinguishing cathepsin E and D activity in antigen-processing organelles pdf

... activity CaD activity TAPACatE activity CaD activity TAPACatE activity CaD activity A BCFig. 5. Distribution of TAPA, CatE and CatD activity in subcellularfractions of the cell lines (A) HaCaT, (B) ... 956–964.10 Tsukuba T, Hori H, Azuma T, Takahashi T, TaggartRT, Akamine A, Ezaki M, Nakanishi H, Sakai H &Yamamoto K (1993) Isolation and characterization ofrecombinant human cathepsin E expressed ... Tsukuba T, Okamoto K, Okamoto Y, Yanagawa M,Kohmura K, Yasuda Y, Uchi H, Nakahara T, FurueM, Nakayama K, et al. (2003) Association of cathepsinE deﬁciency with development of atopic dermatitis.J...

Báo cáo khoa học: A new molecular tool for transgenic diatoms Control of mRNA and protein biosynthesis by an inducible promoter–terminator cassette docx

... PCR was performed on an egfp-containing plasmid (a gift from Dr K. Apt, Martek Biosciences, Columbia,MD, USA) with sense primer SOE-3 (5¢-AAAAATCAACGCTGAACAATGGTGAGCAAAGGGCGAG-3¢) andantisense ... resistance plasmidpCfcp-ble The ble gene [34] (GenBank accession number X52869) wasampliﬁed from pZEOSV (Invitrogen, Carlsbad, CA, USA)by PCR using sense primer 5¢-ATCAAAACAACCAAAATGGCCAAGTTGACCAGTGC-3¢ ... fcp1.9kb using the sense primer 5¢-GATCTTTGCTACGTACGAACG-3¢ and the antisense primer5¢-GCTCTAGAGATATCTAGTCTTTGTGATAAAGAAAATTATG-3¢. The resulting 165-bp PCR product containedan Eco105I restriction...

Báo cáo khoa học: A new clan of CBM families based on bioinformatics of starch-binding domains from families CBM20 and CBM21 potx

... Watanabe A, Iriguchi M, Ishikawa A, KawashimaK, Kimura T, Kishida Y, Kohara M, Matsumoto M,Matsuno A, Muraki A, Nakazaki N, Shimpo S, Sugi-moto M, Takazawa M, Yamada M, Yasuda M &Tabata ... al. A new clan of CBM families58 Nakamura Y, Kaneko T, Sato S, Mimuro M, MiyashitaH, Tsuchiya T, Sasamoto S, Watanabe A, KawashimaK, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M,Matsuno A, ... 181–185.60 Kaneko T, Sato S, Kotani H, Tanaka A, Asamizu E,Nakamura Y, Miyajima N, Hirosawa M, Sugiura M,Sasamoto S, Kimura T, Hosouchi T, Matsuno A, Mur-aki A, Nakazaki N, Naruo K, Okumura S, Shimpo...

Báo cáo khoa học: "A new Approach to Improving Multilingual Summarization using a Genetic Algorithm" pptx

... threshold values are marked with a cross and listed in Table 2 together with the aver-age threshold values obtained after method eval-uation on English and Hebrew corpora. Eachmethod was evaluated ... perform any morphologicalanalysis. with on a daily basis (Filippova et al., 2009), as-sist in the automated classiﬁcation and ﬁltering ofdocuments, and increase search engines precision.Automated ... ever-increasing variety of languages2dic-tates the importance of developing multilingualsummarization approaches. There is a particu-lar need for language-independent statistical tech-niques that...

Tài liệu Báo cáo khoa học: a-Defensins increase lung ﬁbroblast proliferation and collagen synthesis via the b-catenin signaling pathway doc

... luciferase mRNA was used as a control siRNA. The target sequence for luciferase mRNA was 5¢-AACGTACGCGGAATACTTCGA-3¢. The siRNAs were custom-synthesized by Qiagen (Valencia, CA, USA) and weretransfected ... siRNA knock-down of b-catenin The expression of b-catenin was silenced using siRNA tech-nology. The target sequences for the mRNA of b-cateninwere 5¢-AAAGCTGATATTGATGGACAG-3¢. The siRNAagainst ... that a- defensins activate the b-catenin signaling pathway. We then studied the roleof the b-catenin signaling pathway in a- defensin-induced increases in the proliferation and collagen syn-thesis...

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