Báo cáo khóa học: Structural properties of the protein SV-IV potx

... identiﬁed by the massincrease of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... electrosprayMS. Theoretical mass is the mass calculated on the basis of the protein amino-acid sequence. The relative abundance refers to the ratio of the acetylated/unacetylated SV-IV forms. ID, ... 74–90 from the endo-proteinase Glu-C digest of acetylated protein SV-IV. Signal diagnostics of peptide structureare indicated in the ﬁgure.Ó FEBS 2003 Structural properties of SV-IV (Eur....

Báo cáo khóa học: Functional properties of the protein disulﬁde oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulﬁde-isomerase doc

... wasusedasanegativecontrol. The refolding of the scrambled RNase in the presence of Pf PDO seems to be less efﬁcient when usingPDI. However, the temperature of the assay, which islimited by the stability of the protein ... active sites. Refolding of the scrambled RNase in the presence o f P DI was used as apositive control, and the absence of the recovery of the RNase activity in the presence of the thioredoxin fromAlicyclobacillus ... (225 mM)9:1(25 mM). At the end of the incubation, 40 lL of the mixturewas added to each of the ﬁve solutions; these were then leftto react on ice for 5 min. The reaction mixtures w ereanalysed...

Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx

... [1]. The structural retroviral polyprotein (Gag) of HIV-1 forms the immature capsid, and is subse-quently cleaved by the viral protease into severalmature proteins: the matrix, the capsid protein ... residues 146–231 of the intact protein; CACW40A, mutant of CAC with Ala instead of Trp at position 184 of CA; CSA, chemical shift anisotropy; Gag, the structural retroviral polyprotein of retroviruses;NOE, ... Ser146 in the numbering of the intact CA). The CACW40A protein is monomeric, and its structure is similar to that of the subunits in the dimeric, non-mutated CAC, but, in the monomeric form, the segment...

Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-speciﬁc truncated splice variant Mark Wehner and Christian Herrmann doc

... likely due to an elongatedshape of the protein. The same behavior is observed in the presence of GDPÆAlFx. Because of the unexpectedobservation of a monomeric protein species with bothGDP and ... account). The size of the protein complex is onlyslightly increased when nucleotide-free or in the presence of GTP. As with hGBP5ta, the complex isincreased to the size of a tetramer after binding of GppNHp, ... for the lack of GMP formation(see Fig. S2). The similar hydrolytic activities of the a ⁄ b and C-terminally truncated forms of hGBP5 are incontrast to that of the analogous deletion mutant of hGBP1...

Báo cáo khoa học: Structural ﬂexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for speciﬁc substrate recognition pptx

... Switzerland The ﬁdelity of protein synthesis depends on the correctattachment of amino acids to the 3¢-ends of their cog-nate tRNA species by aminoacyl-tRNA synthetases(aaRS), a family of enzymes ... diminishedFig. 5. Model of the acceptor end of tRNASerbound in the activesite of mMbSerRS. The view is focused on the acceptor part of tRNA in the active site of mMbSerRS. The tRNA is shown as ... fold. The HTH fold of one mono-mer is positioned above the catalytic core of the other.Interestingly, Arg267 from the beginning of helix 9 in the HTH fold of one monomer is positioned towardsthe...

Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

... study the secondary structure of the catalytic domain of PKCfand the effect of its substrate, MgATP, and also tostudy the effect of thermal unfolding in the presenceand the absence of MgATP. ... structure of 282 residues of a total of 344have been solved [19]. The catalytic domain of PKChpossesses a high degree of homology ( 80%) with the catalytic domain of other isoforms of PKC belongingto ... When D2Obuffer was used, the protein was incubated overnight at4 °C to maximize H–D exchange. To study the IR amidebands of the protein in the presence of MgATP, the protein was prepared in...

Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

... for the p eptide.NMR s pectroscopy was further utilized to explore the structural properties of Nogo-40. The very narrow reson-ance dispersion of amide protons ( 0.7 p.p.m) and the lack of ... Zn2+.Furthermore, the d etermination of the Nogo-40 solutionstructure offers a starting point for further understanding the interaction between NgR and Nogo-40, and forfuture designs of molecules ... Song1,21Department of Biological Sciences and2Department of Biochemistry, National University of Singapore, Singapore The recent discovery of the Nogo family of myelin inhibitorsand the Nogo-66 receptor...

Báo cáo khoa học: Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622 potx

... of note that the structure of the LPS O-antigen of F. columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21],which is a linear polymer of ... andJ1,28.8 Hz. Thecorrelation of H2C tothe C2C at55.4 p.p.m.is consistent with the presence of a C2 acetamido substituent,and the lack of a proton at C6, considered in conjunctionwith the long-range ... conclusion, the NMR analysis of the native O-PS showed that the chemical shifts of the linkage position carbon atoms C4A,C3B,andC4Cexperience significant deshielding, further confirming the linkage...

Báo cáo khoa học: Structural properties of semenogelin I docx

... thirdexpresses the untranslated 3¢ part of the mRNA [11].Neither the primary structure, nor the repetitive ele-ments of the intact semenogelins are similar to motifsseen in other proteins, and thus the ... by exposure to Zn2+. The observation that high concentrations of ureadissolve the gel strengthens the assumption that the structure of SgI (as the dominating protein in the coagulum) is important ... creation of the gelatinous coagu-lum upon ejaculation. The gel is liqueﬁed underdenaturing conditions, which indicates that the confor-mation of the proteins is important for the integrity of this...

Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf

... the rate of the conformationalchange, k3H the hydride transfer and k4 the rate of the product-off step. Hence, in these circumstances, the steady-state KMis expected to be smaller than the apparent ... of either substrate or cofactorwith varied concentrations of the other. The experi-ments were performed at different, fixed concentrations of either lactate or NAD+. A subsequent set of experi-ments ... above, hence the tag did not influence the kin-etic behaviour of the enzyme to any measurable extent.Equilibrium binding affinity of NADH The binding of NADH to the active site of dehydro-genases...

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