Báo cáo khoa học: Crosstalk between Src and major vault protein in epidermal growth factor-dependent cell signalling docx

... regulatory and ⁄ or substrate proteins and areintegrated into cell signalling pathways and cell cycleevents [23]. The UD, Src homology 3 and Src homology 2 (SH2) domains in Src are key bindingsites ... a Src SH2binding protein. We observed that MVP interactswith Src in mammalian cells and inhibits the activityof Src tyrosine kinase.ResultsIsolation of MVP as Src SH2 interacting protein by ... of Src in normal and trans-formed cells [22]. Compelling evidence indicates that Src- binding proteins can regulate Src activity [23].While a number of interacting proteins that upregu-late Src...

Báo cáo khoa học: Interactions between metals and a-synuclein ) function or artefact? pptx

... of proteins including b-synuclein and c-synuc-lein [3]. In contrast to a-synuclein, these two proteinsdo not appear to aggregate or form ﬁbrils [26]. How-ever, b-synuclein is known to inhibit ... whether metal binding to a-synuc-lein is an artefact or not. However, the new evidencesuggesting that a-synuclein is a metal binding protein is intriguing and is likely to result in a robust, ... the potential binding site indicated that aggrega-tion of the protein was only initiated if the C-terminuswas intact [56]. This suggested that the C-terminus wasthe principal binding site for...

Báo cáo khoa học: Molecular cloning, expression and characterization of protein disulﬁde isomerase from Conus marmoreus pdf

... its C-terminus. The maturecPDI protein has a calculated molecular mass of54 913.7 Da and an isoelectric point of 4.6. The cPDIcontains four thioredoxin domains and an acidic C-ter-minal tail ... conotoxins have been thoroughlyinvestigated, and two distinct folding mechanisms havebeen unveiled [20]. Through comparison of foldingkinetics and thermodynamics, the folding mechanismof tx3a and ... Oulu,Finland). Ni2+-chelating Sepharose Fast Flow resin and Q-Sepharose Fast Flow resin were obtained from Amer-sham Biosciences (Arlington Heights, IL, USA). TheRACE kit was obtained from Invitrogen...

Báo cáo khoa học: Insights into substrate and product trafﬁc in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt

... most 5% of the traffic, with the majority of traffic pass-ing through the main entrance. In mutants Trp83Ala and Trp83Glu,ligands that close the main entrance do not inhibit substrate hydrolysisbecause ... DmAChE(residues and labels in green) and Torpedo californica acetylcholin-esterase (residues and labels in fushia). Residues are representedby sticks. The hydrogen bonds involving the hydroxyl ... of choline outside the gorge is blocked when thePAS is occupied and choline stays inside the activesite, resulting in inhibition [9]. Parameter b is signifi-cantly different from zero, and no...

Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot

... containing CLP and CLAP witha phosphoprotein stain did not reveal phosphate additionsto these proteins. Only lanes in the gel containing the knownphosphoproteins ovalbumin, b-casein and pepsin ... fasciclin domains in CLAPwith other fasciclin I containing proteins is clear (Fig. 9).UsingBLAST(http://www.ncbi.nlm.nih.gov/BLAST) toidentify related proteins, a putative cell adhesion protein from ... nucleotideÓ FEBS 2004 Cathepsin L and cell adhesion protein in Artemia (Eur. J. Biochem. 271) 4023binding domains, so the presence of a GTP/ATP bindingregion in CLAP may indicate an energy dependent...

Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx

... structural and func-tional determinants of the protein protein interaction between v-KIND and MAP2 (i.e. the MAP2-bindingcore module in v-KIND and the v-KIND-binding coremodule in MAP2) across ... with GST-CD2 (MAP2 v-KIND binding core).Input (cerebellum)GSTv-KIND KIND1v-KIND KIND2Spir-2 KINDPTPN13 KINDInput (MAP2)GSTv-KIND KIND1v-KIND KIND2Spir-2 KINDPTPN13 KINDPD from cerebellar ... February2011)doi:10.1111/j.1742-4658.2011.08085.xThe kinase noncatalytic C-lobe domain (KIND) is a putative protein protein interaction module. Four KIND-containing proteins, Spir-2 (actin-nuclearfactor), PTPN13 (protein tyrosine phosphatase),...

Tài liệu Báo cáo khoa học: Mechanisms of obesity and related pathologies: Androgen deﬁciency and endothelial dysfunction may be the link between obesity and erectile dysfunction pptx

... Relationship between BMI, totaltestosterone, sex hormone-binding-globulin, leptin,insulin and insulin resistance in obese men. ArchAndrol 52, 355–361.19 Wannamethee SG, Shaper AG, Morris RW &Whincup ... the production of in ammatory cytokines (e.g.IL-6, TNFa) as well as lipoprotein lipase, angiotensinogen, free fattyacids, resistin, leptin, lactate, PAI-1, insulin and adipsin, and reduces the ... plasma androgen levels [14–19]. In thisminireview, we discuss the link between obesity and ED by focusing on the potential pathophysiologicallinks including obesity-related androgen deﬁciency and obesity-induced...

Tài liệu Báo cáo khoa học: Toggle switches, pulses and oscillations are intrinsic properties of the Src activation/deactivation cycle doc

... loops and occur at constant activities of Src inhibitors and activators, such as C-terminal Src kinase and receptor-type protein tyrosine phosphatases. We demonstrate that C-terminal Src kinase and ... ﬁbronec-tin-coated beads adhering to the cells. As ﬁbronecitonbinds to integrins, the local pulling force stimulatedintegrins that subsequently activated Src. Intriguingly,the local Src activation ... C-terminal Src kinase; FAK, focal adhesion kinase; MAPK, mitogen-activated protein kinase; PTP1B, protein tyrosine phosphatase 1B;QSS, quasi steady-state; RPTP, receptor-type protein tyrosine...

Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf

... the cellswas determined. The cells included the K562 cell line and its Pgp over-expressing subline (K562 ⁄ ADR), and the GLC4 cell line and its MRP1over-expressing subline (GLC4 ⁄ ADR).Innate ... cells was determined as described in Fig. 1. The cells included the K562 cell line and its Pgp over-expressing subline(K562 ⁄ ADR), the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ... 8. NBD-Cl-sensitive TMRM uptake into various MDR cells and their wild-type cell lines. Cells were incubated for 15 min at 23 °Cinamedium containing CCCP (1 lM) in the absence (control) or presence...

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... (1996) Activator anion binding site in pyridoxal phosphorylase b: The bind ing of phosp hite, phosph ate, and ﬂuorophosphate in the c rystal. Protein Sci. 5, 2416–2428.26. Schinzel, R., Palm, D. ... Enzyme–oxyanioninteractions occur a t a protein site different from the activesite, and thermostabilization is the result of a protein conformational change induced by the binding event.Residues involved ... Psik, Alexandra Schwarz and Bernd NidetzkyInstitute of Biotechnology and Biochemical Engineering, Graz University of Technology, AustriaUsing 0.4Mimidazole citrate buﬀer (pH 7.5) containing0.1...

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