Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... generaltranscriptional co-activators that contain histone and transcription factor acetylation activities [30]. In addi-tion, CBP contains a number of protein-bindingdomains that mediate transcription ... catalyze mono-, di- and trimethyla-tion of H3K4, regulation of which can have distinctfunctional consequences. MLL1 contains a number of conserved functional domains that work together for the ... [31]. The KIX domain of CBP is a structural platform that is capable of binding severaldifferent families of transcriptional activators [30], and evidence indicates that the KIX domain has the abilityto...

Tài liệu Báo cáo khoa học: Bacitracin is not a speciﬁc inhibitor of protein disulﬁde isomerase pptx

... further study the mechanism of action of bacitra-cin, the reduction of insulin was analyzed in the pres-ence of the PDI a domain and DsbA, which arecapable of catalyzing the oxidation and ... domains, a, b, b¢, and a , a linker region xbetween the b¢ and a domains, and a C-terminal acidicextension. The a and a domains contain the CGHCactive site motif, and are sufﬁcient alone ... change in activity upon the addition of bacitracin (P > 0.5).For further study of the potential mechanism of action, the reaction conditions of the assay were var-ied. The removal of EDTA...

Tài liệu Báo cáo khoa học: "Topological Dependency Trees: A Constraint-Based Account of Linear Precedence" ppt

... ‘oblig-atory auxiliary flip’.(18) (dass)(that)MariaMariaeinen a Mannmanhathasliebenlovekönnencan(that) Maria was able to love a man(19) (dass) Maria hat einen Mann lieben können(20)*(dass) ... infinitival inflec-tion, yet acts as a complement of the perfectizerhaben, which syntactically requires a past partici-ple. Only modals, AcI-verbs such as sehen and lassen, and the verb helfen can appear ... Lexicon isdisplayed in Figure 1 where the 2 features cats and valencyID of concern to ID trees are groupedunder table heading “Syntax”. Finally, cat and valencyIDassign a category and anR...

Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc

... (LDA).Thisstatisticalmultivariate method is supervised. It searches for the variables containing the greatest interclass variance and the smallest intraclass variance, and constructs a linearcombination ... greatestpart of the original data information.In the second part of our work, we focused on the biochemical information available i n infrared s pectra, and we found that infrared spectra of resistant ... cells a d ecrease of the absorptions assigned to fatty a cids and phospholipidsrelative to their protein content. This qua ntitative changewas accompanied by qualitative modiﬁcation, as the methyl/methylene...

Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... 5¢-CAGCCGCCGTAGAAGTTGTAAGTCATCGCAAAG-3¢,5¢-CGCCGCCACCAGGGAACATCCAGTCAATATCTAC-3, and 5¢-GCCGCCACCAGGGAATTGCCAGTCAATATCTAC-3¢, respectively.Conﬁrmation of the mutated nucleotides by automatedsequencing was carried ... In the case of the E315Q mutant, an additional faint band was alsoseen at an Mr of  43 000. This band appeared as a degradation product during freezing and thawing of the protein that was ... reaction with chitinase A from V. carchariae .A combination of HPLC and ESI MS allowed the separ-ation of a and b anomers and all chitooligosaccharideproducts to be monitored simultaneously. At the...

Tài liệu Báo cáo khoa học: Transactivation properties of c-Myb are critically dependent on two SUMO-1 acceptor sites that are conjugated in a PIASy enhanced manner pptx

... basal level of sumoylation was greatly enhancedby cotransfection of PIASy, an E3 ligase for SUMO-1. The functional consequence of abolishing sumoylationwas enhanced activation both of a transiently ... principalsumoylation site and K503 a secondary one. Both sites areimportant for transcriptional activity and their mutationcauses a large enhancement of Myb-dependent transactiva-tion. Sumoylation ... suspension was dividedwithonethirdusedforpreparationofthetotalfraction(T)andtheremaining two-thirds used to make the soluble and nuclear matrixfraction. Total protein concentration of the soluble...

Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 reﬁned models suggest environment-dependent induction of alternative bent ⁄extended resting states doc

... tailseparation was in accordance with both the ET and hydrodynamic data of primed a IIbb3. Our revised mod-els further support an alternative view of the confor-mational states and mechanism ... thermodynamic cost of such a hugeconformational change could still be manageable, itsphysiological need is unclear, and alternative explana-tions for the role of the bent conformation, such as the ‘packaging ... (panels D and I) and A2 bB3-eots (panels E and J), are shown as ribbons (protein only, panels A E) and as surface(protein) and space-ﬁlling (carbohydrates and OG moieties) representations (panels...

Tài liệu Báo cáo khoa học: Top-down MS, a powerful complement to the high capabilities of proteolysis proteomics pdf

... localized on the backbone, with little accompa-nying cleavage of weaker side-chain modifications suchas glycosylated [37] and phosphorylated structures [38] (and even of noncovalent bonding and conformationaltertiary ... mod-ifications [42].Fig. 9. ECD spectral data from the RNase A deamidation samples of Fig. 8. Deamidation at an individual residue of a specific product causes a 1 Da increase in any fragment ion containing ...  4 Da, the decrease of the molecular mass value. The most proba-ble reason for a 2 Da decrease is the formation of anS–S bond; although this was totally unexpected and unprecedented, the top-down...

Tài liệu Báo cáo khoa học: Electrical properties of plasma membrane modulate subcellular distribution of K-Ras ppt

... experiments and theoreticalanalyses suggest that membrane association and plasma membrane targeting of K-Ras are a conse-quence of the electronegative sensing function of the C-terminal domain of this ... Biophys Acta401, 509–529.42 Jacobson K & Papahadjopoulos D (1975) Phase transi-tions and phase separations in phospholipid membranesinduced by changes in temperature, pH, and concentra-tion ... Identification and purifi-cation of aminophospholipid flippases. Biochim BiophysActa 1486, 108–127.56 Toro M, Arzt E, Cerbon J, Alegria G, Alva R, Meas Y& Estrada S (1987) Formation of ion-translocating...

Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... product concentration, vz and vsare the initial and steady -state velocities, respectively, and k is the pseudo-ﬁrstorder rate constant describing the presteady state of the reaction. Based on ... concentration and kass and kdissare the associ-ation and dissociation rate constants, respectively. k wasdetermined for four to six inhibitor concentrations. Linearregression analysis of the ... act as one of the main means of regulation [8]. The best characterized are the cystatins,which comprise a superfamily of evolutionarily relatedproteins, each consisting of at least one domain...

Xem thêm