Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas ﬂuorescens potx

... with the gene of the benzaldehyde lyase and for helpful discus-sions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their helpwith data collection and ... Journal compilation ª 2005 FEBS Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas ﬂuorescens Tanja G. Mosbacher1, Michael Mueller2 and Georg E. Schulz11 Institut ... consist of a cen-tral six-stranded parallel b-sheet ﬂanked by a varyingnumber of a-helices. Residues involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of...

Báo cáo khoa học: Structure and topology of the transmembrane domain 4 of the divalent metal transporter in membrane-mimetic environments docx

... field[32]. The final 16 lowest energy structures of DMT1-TM4 inboth SDS (pH 6.0) and TFE were chosen to represent the solution structures of the peptide, as shown in Fig. 3. The quality of the final structures ... shifts for DMT1-TM4 in TFE and SDS.Ó FEBS 2004 Structure and topology of TM4 of DMT1 (Eur. J. Biochem. 271) 1951 Structure and topology of the transmembrane domain 4 of the divalent metal transporter ... suggeststhat the C-terminus of the peptide is partially embedded intoSDS micelles at pH 4.0, which may therefore block the entrance of either solvent molecules or metal ions. The movement of the C-terminus...

Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf

... than the isofunctional enzymes from other bacteria (Fig. 2).Expression of ArCMLE1Comparison of the sequence of ArCMLE1 with that of the recently published crystal structure of the CMLE from ... in the mutant. First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structure of the protein and therefore the ... catalysis. The alignment of the small CMLEs from different members of the Rhizobiales with these sequences demonstratedthat in A. radiobacter S2 (and the other member of the Rhizobiales), the amino...

Báo cáo khoa học: Structure and activity of the atypical serine kinase Rio1 doc

... with the uncomplexed protein. Com-parisons of the structure of Rio1 with the previously determined structure of the Rio2 kinase defined the minimal RIO domain and the distinct fea-tures of the ... Asp99 and Phe102,Met104 and Ile107, and Ser108 and Glu111, whichmarks the start of aC. The entire flexible loop packsbetween the N-terminal portion of aC and part of the C-lobe (Figs 2A and 3A). The ... part of the adenine ring of ATP (Fig. 2B). No equivalent fea-ture was seen in the structure of Rio2 or in any otherkinase structures that we examined. As a result of the presence of the flap, the...

Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

... C (1989) The limits of the DNase I-sensitive domain of the human apolipoproteinB gene coincide with the locations of chromosomalanchorage loops and deﬁne the 5¢ and 3¢ boundaries of the gene. ... Since then there has been an explo-sion of papers on the multitude of chromatin modiﬁca-tions and the factors that can either create orrecognize them. We now have a very detailed picture of the ... bp intervals. The lighter colour shadesdepict the bottom half of the nucleosome, and the exploded viewbelow the octamer depicts the arrangement of the histones con-tacting 73 bp of DNA within...

Tài liệu Báo cáo khoa học: Structure and function of plant aspartic proteinases pptx

... N-terminal of the mature enzyme and by the ÔflapÕ. The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionicinteractions established between Lys11/Tyr13 of ... hybridization, in the digestive glands of the pitchers, the trapping organs of the plant [6]. Usingimmunohistochemistry and immunogold transmission EM,APs purified from the flowers of the cardoon Cynaracardunculus ... cleft. Nevertheless,only the structural characterization of procardosins and Fig. 2. Ribbon representation of the crystal structures of cardosin A (A) and prophytepsin (B). (A) Structure of mature...

Báo cáo khoa học: Structure and function of KH domains docx

... in which the b1-strand and b2-strand are adjacent and parallel to each other, and the b¢-strand is adja-cent and antiparallel to the b1-strand (Fig. 1). The length and sequence of the variable ... data from other KH domains to the KH1–KH2 domains of FMRP. The structure of the tandem KH1–KH2 domains of hFMRP provided the ﬁrst crystallographic description of the structural environment of the ... single-stranded conformation across one face of the KHdomain, between the a1-helix and the a2-helix and GXXG on the ‘left’, and the b2-sheet and the vari-able loop on the right (Fig. 4A). Together, thesesecondary...

Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identiﬁcation of two active site cysteines pptx

... comparable to the hexokinase subunits [24]. Onelobe consists of the phosphate1 subdomain and the predicted a-helix I. The other lobe consists of the phos-phate2 and the adenosine subdomains, the predicted ... fitted into the glucokinase model using RasMol software. Figure 5 shows the predicted structure of GlcNAc kinase with the localiza-tion of the five ATP-binding subdomains and the secondarystructures ... in the catalytic mechanism of GlcNAc kinase. C131 and C143 seem to bedirectly involved in the transfer of the c-phosphate from ATP to the hydroxyl group at C6 of GlcNAc. This isconfirmed by the...

Báo cáo khoa học: Structure and function of human a-lactalbumin made lethal to tumor cells (HAMLET)-type complexes pptx

... estimation of the hydrodynamic radii of HAMLET (Rh= 26.9 A˚),which is intermediate of the hydrodynamic radii of the acidic molten globule state of a-lactalbumin(Rh= 20.9 A˚) and the theoretically ... fully understood. The active group of the matrix, the DEAE group, is posi-tively charged and might therefore bind negative mole-cules. At pH 8.5 (the pH of the conversion step) the fatty acid ... describ-ing the form and function of novel complexes such asHAMLET and ELOA, we have provided new evidencethat a loss of native structure can endow proteins and their complexes with distinct and beneﬁcial...

Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

... Among the 2314 mutants, 1148 were localized in the central coredomain of the protein and were used for training and evalua-tion of our prediction algorithm. Of the eight promoters, westudied the ... prediction of the severity of p53 mutants. The method was trained on a set of known mutants and subsequently evaluated on anotherset. The method is based on parameters reﬂecting the bio-chemical and ... powerful method, and several algorithms have been developed recently [4–7]. The major drawback of these analyses is the lack of information regarding the activity or loss of activity of the target...

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