Báo cáo khoa học: The role of interface framework residues in determining antibody VH ⁄ VL interaction strength and antigen-binding afﬁnity pptx

... while the OS-fitness was close to 1.Relationship between antigen binding and VH ⁄ VL interaction The relationship between the indexes of antigen-bind-ing affinity and VH ⁄ VL interaction strength ... correlation between the antigen-binding affinity and the VH ⁄ VL interaction, especially in the presence of aset of particular VL residues. The effect of the H39 mutation on the wild-type variable ... strong affinity, yet a veryweak VH ⁄ VL interaction strength, in the absence of antigen. To investigate the possible relationship between antigen-binding affinity and VH ⁄ VLinter-action strength, ...

Báo cáo khoa học: "The Role of Lexico-Semantic Feedback in Open-Domain Textual Question-Answering" ppt

... example, in the case of questionQ209: “Who invented the paper clip ?”, the expected answer type is PERSON and so is the subject of the verb invented , lexicalized as the nominalization inventor. ... mountain; (b) lava IS-PART of vol-cano, and moreover it is a part coming from the inside; and (c) fragments of lava have all the prop-erties of lava, the following question semanticform and ... characterized by the need forcontextual information, brought forward by ref-erence resolution. In the following example, achain of coreference links Bill Gates and Mi-crosoft founder in the candidate...

Báo cáo khoa học: The role of glucose 6-phosphate in mediating the effects of glucokinase overexpression on hepatic glucose metabolism pdf

... selective inhibitor of Glc6P hydrolysis [31],for evaluating the role of Glc6P, we determined the effect of different inhibitor concentrations on the Glc6P content and the rate of glycogen synthesis in hepatocytes ... control of glycolysis and glycogen synthesis by glucokinase overexpres-sion. The validity of S4048 for testing the role of Glc6P was supported byits lack of effect on glucokinase binding and its ... 2005 The Authors Journal compilation ª 2005 FEBSlevels through the interaction of glucokinase with bind-ing proteins [5,6]. The glucokinase regulatory protein(GKRP) is a speciﬁc inhibitor of...

Báo cáo khoa học: The role of cytochrome P450 monooxygenases in microbial fatty acid metabolism pdf

... tightbinding afﬁnity was demonstrated towards lauric acid,resulting in the synthesis of mainly x-1 hydroxylatedlauric acid, suggesting a role in the lipid oxidativemetabolism. Interestingly, the ... required forbreaking the plant’s defence but, on the other hand,alkanes serve as nutritional input during the initial col-onization steps. Insect cuticle consists of protein and chitin, and is covered ... fumonisin is suggested tobe carcinogenic. The 17 fumonisin biosynthetic genesare located in a gene cluster and three of them areP450 enzymes. The FUM6 protein (CYP505B1) inter-venes in one of the...

Báo cáo khóa học: The role of N-linked glycosylation in the protection of human and bovine lactoferrin against tryptic proteolysis pdf

... NuijensPharming, Archimedesweg, Leiden, the NetherlandsLactoferrin (LF) is an iron-binding glycoprotein of the innate host defence system. To elucidate the role of N-linkedglycosylation in protection of ... herein further elucidate the role of N-linked glycosylation in the protection of lactofer-rin against tryptic proteolysis. It appeared that glycosylationat Asn281 protects bLF against trypsin. ... lactoferrin where the protein has to survive the harsh environment of the gastro-intestinal tract. Thus, on the basis of this study, rhLF maybe preferred over bLF in oral applications of lactoferrins...

Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

... (rotationangle ‘r’) and variation of the inclination (inclination angle ‘i’) withrespect to the plane of the membrane (parallel to the xy plane).Cter and Nter are the C-terminal and N-terminal structural ... onto the axis z, and (b) to conserve the minimal distancebetween protein and membrane at 3.5 A˚. The EIE, i.e., the electrostatic contribution to the bindingenergy (DGbindingR ⁄ M) of RNase ... regions,respectively, of the two A and B monomers of the dimeric RNase. The black circle between the monomers indicates the mass center of the dimer.E. Notomista et al. Electrostatic interactions and antitumor...

Tài liệu Báo cáo khoa học: The role of antioxidants in the cytotoxicity of chemotherapeutic drugs pptx

... undergoes fusion with the membrane of intra-endosomal vesicles contained within MVBs,thereby releasing the nucleocapsid into the lumen of these vesi-cles. Then, the capsid uses these intra-endosomal ... studies in our group demonstrated the involvement of the in ammatorykinase JNK and endoplasmic reticulum (ER) stress as eventsinvolved in both sensing and relaying stress signals and disturb-ing ... Thus, in the absence of recombination, the cells become hypersensitive to killing bymethylating agents and arrest already in the ﬁrst G2. We arecurrently searching for the structures of the recombination...

Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx

... likely that the N-terminal domain is involved in protein–protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspeciesinteractions are incompatible.From ... and the effect of the absence of intact complex I, but the results are toopreliminary in this regard.Heterologous expression of the ESSS-HA subunit The localization of the ESSS subunit in the ... mutantsrevealed chain termination mutations. In two of thesemutants the protein i s truncated at the C-terminus of the targeting sequence; the m utants are null mutants for the ESSS subunit. There is...

Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafﬁcking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efﬁcient GABA-uptake activity of the GABA transporter pptx

... involved in the regulation of the stability and trafﬁcking of GAT1. It has beenreported that a variety of molecular chaperones and folding enzymes assist the folding of newly synthesizedproteins in ... N-linked oligosaccharide side-chainsare important for the GABA transport activity.1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into the role of the terminal ... boiling for 4 min in SDS sample buffer, and then ana-lyzed by SDS ⁄ PAGE and western blotting. Either anti-GFP mAb MAB2510 or anti-GAT1 pAb was used for the immunostaining. The protein bands...

Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf

... furtherinvestigate the role of the SEA domain in cleavage of membrane-tethered mucins we carried out site-directedmutagenesis of the FRPG ⁄ SVVV site and evaluatedhybrid mucins in which the ... the role of this domain in the processing of membrane-tethered mucins. The mech-anism by which the heavily glycosylated portion of these molecules is released at epithelial surfaces within the ... Model of SEA domain of MUC1. Ribbon representation of the modelled SEA domain of human MUC1. The model consists of three a-helices and six b-strands forming an a ⁄ b sandwich fold. The FRPG ⁄ SVVV...

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