Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx

... 273 (2006) 2127–2138 ª 2006 The Authors Journal compilation ª 2006 FEBS The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction Greta Forlani, ... neither the isolated DH nor the PH2 domain bound microtubules. In the absence of microtubules, none of the recombinant proteins wasfound in the pellet fraction. These ﬁndings indicatethat the ... performedto test whether the DHPH2 module is the only regionresponsible for the interaction of the entire RasGRF1 molecule with microtubules. Pure microtubules wereincubated for 20 min with RasGRF1- containing...

Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt

... 270) 4543 the nucleotide exchange during the peptide translocationprocess. The nonreciprocal behaviour of the chimeric TAP-NBDssuggests that the C-terminal regulation of nucleotide interaction ... model for the transport cycle of the active TAP variant 2–1C2. The initiation step and the progress of the transport cycle aresimilar to the hypothesized peptide translocation model of the wild-type ... thatretained the normal ADP binding capacity but lost the conformational properties allowing the access of freeMgATP to the nucleotide binding pocket and accordinglyÓ FEBS 2003 Regulation of the nucleotide...

Báo cáo khoa học: The human pyridoxal kinase, a plausible target for ginkgotoxin from Ginkgo biloba docx

... due to the ingestion of Ginkgo remedies. However,they neither specify the respective remedies, nor dothey mention their composition. Therefore, the possi-bilities of overdosage or interactions ... the antivitamin character of both compoundsand explains a depletion of cofactor formation in the organism in the presence of the antivitamins. The kinetic data (Table 1) are a reﬂection of the structural ... relevantconcentrations of the toxin were tested in vitro [8]. Itfollows that the interaction between ginkgotoxin andPKH is a rather speciﬁc process which affects the keyreaction for the supply of the human...

Báo cáo khoa học: The role of interface framework residues in determining antibody VH ⁄ VL interaction strength and antigen-binding afﬁnity pptx

... absorbance, others showed adecreased or diminished antigen-dependency. To evalu-ate the OS-fitness of the clone, the ratio of the specificabsorbance in the presence of 10 lgÆmL)1HEL to thatin the ... VL interaction strength and the OS-fitness. When H39 was Lys (K), as in HyHEL-10, the VH⁄ VL interaction in the absence of antigen wasgenerally weak, while the OS-fitness was high. On the Fig. ... the wild-type scFv, which resulted in a 0.58-fold equilibrium association constant. Apparently, the mutation to strengthen the VH⁄ VL interaction wasalmost as effective as tethering by the...

Báo cáo khoa học: The cytochrome P450scc system opens an alternate pathway of vitamin D3 metabolism docx

... Aminogluthimide inhibition showed that the three majormetabolites, but not the others, resulted from P450scc action. It thereforeappears that non-P450scc enzymes present in the adrenal cortex to someextent ... tools for the characterization of secoster-oid products. Furthermore, we tested vitamin D3 bio-transformation by isolated mitrochondria from the adrenal gland, the tissue expressing the highest ... 25-hydroxycal-ciferol by hydroxylation at C-24. At the autocrine orparacrine levels the biological signiﬁcance may extendto the skin, where both the synthesis and metabolismof vitamin D3 occur [1,3,5],...

Báo cáo khoa học: The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs pdf

... Taken together,these results show that the ROQ domain is essentialfor Roquin’s repression of Icos mRNA.Roquin binds Icos mRNATo determine whether Roquin directly binds the por-tion of the Icos ... indi-cated by a wedge above the relevant lanes, and the absence ofany added protein is indicated by ()). The position of the free RNAand of the loading slots is indicated to the right of each gel.Roquin ... granules is shared by the MNAB proteinencoded by the other member of the mammalian Rc3hgene family, Rc3h2. Stress granule localization is likelyto be critical for the function of these proteins,...

Báo cáo khoa học: The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed docx

... genes with the human cathepsin D gene. The top line of the figure shows the arrangementof the functional regions of the plant aspartic proteinase genes like in Fig. 1. Below that are the representative ... fourtimes stronger in the tissue from the light when compared tothetissuefromthedark.Thiswasthesameorderofinduction detected by the whole cDNA with the same RNA(2J2 in Fig. 5). Thus, the AtPasp A1 ... at the first site in the AtPasp A2 mRNA was confirmed bysequencing an RT-PCR product from the AtPasp A2 gene(data not shown), while the splicing at the second site wassupported by the size of the...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... activating a guanine nucleotide exchange factor with activity towards Ras.ResultsHEK293 cells express the guanine nucleotide exchange factor Ras-GRF1 The guanine nucleotide exchange factor Ras-GRF1 ... N-terminal deletions of the full-length 140 kDa protein. The physiological role of the guanine nucleotide exchange activity of the truncatedforms is not known as they are missing the Ca2+⁄CaM-binding ... calmodulin; EGF, epidermal growth factor; Epac, exchange protein directly activated by cAMP;ERK, extracellular signal-regulated kinase; GEF, guanine nucleotide exchange factor; GPCR, G protein-coupled...

Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc

... compared the resultswith the rates of the i sotope exchange under t he sameconditions. We h ave found that in the L-phenylalaninereaction the exchange o f t he abstracted proton in the activesite ... in the active site; (b) the statistical factor taking account of the ratio of protons and deuterons on the considered groupwhen the latter is polyprotic; (c) the degree of restriction of the ... [15,16] the abstraction of the a-proton is most probably effected by the e-amino group oflysine 257, which f orms the aldimine bond with the carbonylgroup of the cofactor, PLP, in the holoenzyme...

Báo cáo khoa học: The propagation of hamster-adapted scrapie PrPSc can be enhanced by reduced pyridine nucleotide in vitro pdf

... Calculat-ing the signal intensity of the reactions of 0 and10 lm NADPH revealed that the efficiency of PMCAwas increased by  2.59-fold in the presence ofNADPH. To evaluate whether the effect ... requiredfor the same amplification.PrPScgenerated from PMCA in the presence ofNADPH had further propagation abilityTo determine whether newly generated PMCA PrPScby addition of NADPH could further ... NADPH and the other added to an equal volume ofNaCl ⁄ Pi. After 24 cycles PMCA as the first round, 10 lL of productwas mixed with 90 lL of fresh NBH for the next round of PMCA. The five left-hand...

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