Báo cáo khoa học: Functional association of the AAA complex and the peroxisomal importomer potx

... electrophoresis.Taken together, the data demonstrate that the AAA complex and the importomer do physically interact at the peroxisomal membrane. Association of the importomer and the AAA complex in import ... FEBSinteraction of the membrane-bound AAA complex and the importomer. Therefore, the protein complexes iso-lated with protein A fusions of Pex1p, Pex6p and Pex15p were tested for the presence of peroxins of ... thatPex1p and Pex6p form a complex with Pex15p at the peroxisomal membrane. The core complex of Pex1p, Pex6p and Pex15p isassociated with the importomer The recent discovery of the involvement of AAA peroxins...

Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx

... addition, strengthen the hypothesis of the involvement of Ki-1 ⁄ 57 in pre-mRNA processingevents. The GEMS are regions enriched with the survival of motor neurons (SMN) protein complexes and are con-sidered ... NationalInstitute of Mental Health, Bethesda, MD, USA). The intensities of all isoforms were summed, set as 100%, and used to normalize the intensity of each band.Microscopy analysesFor the subcellular ... suggest-ing that these proteins might form complexes in vivo. The pan-antibody against hnRNPQ recognizes the isoforms hnRNPQ1, hnRNPQ2, and hnRNPQ3, and the antibody against SFRS1 ⁄ 9 recognizes the splicingfactors...

Báo cáo khoa học: Functional transitions of F0F1-ATPase mediated by the inhibitory peptide IF1 in yeast coupled submitochondrial particles pdf

... hydrolysis and converts the latent form of IF1-free F0F1-ATPase back to the active form. The rela-tionship between the diﬀerent steps of the catalytic cycle, the mechanism of inhibition by IF1 and ... determine the afﬁnity of the latent state (E ¼ in the above scheme) of the ATPase for IF1:Kdis %1 lM.Thisvalue is much higher than the Kdfor the IF1–ATPaseinteraction in the absence of D~lHþ,whichis% ... IF1 and D~lHþon the formation of the latentform in the presence of MgATP. These three effectorsmust be simultaneously present to stabilize the closed stateleading to the latent form. The...

Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-ﬂuorouracil in Caenorhabditis elegans docx

... essentialfor the synthesis of RNA and biomembranes and isinvolved in the regulation and function of the cardio-circulatory, reproductive, nervous and respiratory sys-tems [7]. Furthermore, it ... PEIcellulose sheets with 4 lL of nonradioactive tracer (100 lg of 5-FU and 100 lg of uridine for the UP assay mixture;100 lg of 5-FU and 100 lg of deoxyuridine for the TPassay mixture). After ... several uridine kinases exist in the C. ele-gans genome. Three of these were selected on the basis of length and sequence homology, and their expressionpatterns and enzymatic activities were characterized.Deletion...

Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... inhibit the LBS function of either K1[34] or K4 [35] and have used them to analyse, in functional isolation, the role of these LBS on plas-min(ogen) binding and activation. The effect of thesemAbs ... determine the effect of the mAbs 34D3 and A10.2 onplasminogen activation, the fibrin plates and the cells wereincubated with different concentrations of these mAbs in the presence of fixed concentrations ... nonspecificeffects of intact mAbs, including the reproduction of similar results with their Fab fragments and the absence of effect on plasminogen binding and activa-tion in the presence of the unrelated...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... R105H-fwd:5¢-GCTAAAAATAATGGAGCACTCCATTTTTAGCGCTCGC-3¢ . R105H-rev:5¢-GCGAGCGCTAAAAATGGAGTGCTCCATTATTTTTAGC-3¢. R105K-fwd:5¢-GCTAAAAATAATGGAGAAATCCATTTTTAGCGCTCGC-3¢. R105K-rev:5¢-GCGAGCGCTAAAAATGGATTTCTCCATTATTTTTAGC-3¢Sequence ... in hydrogenbonding. The result that E52Q did not show any activitymust therefore be a consequence of the reactivity of the functional group and further support the hypothesisthat E52 acts ... for the chemotherapeutic treatment of cancer and viraldiseases because they catalyse the addition of the ﬁrstphosphate group to the nucleoside analogue. Thisprimes the nucleoside for further...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... DEC1 with BMAL1 and the bin ding to the E-box.Substitution of Arg65 for Ala abolished the interaction of DEC1 with BMAL1 and the binding of DEC1 to the E-box.However, substitution of His57 for ... increase in the mutantmice, the disruption of DEC1 might be compensated byDEC2. Further investigation is required to clarify the functions of DEC1 and DEC2. The suppressive activities of DEC1 and DEC2 ... levels of expression. The transcriptional activity of FLAG-BMAL1, and the suppressive activity of FLAG-DEC1, were s imilar to those of intact BMAL1 and DEC1, respectively (Fig. 1A,C). These ﬁndings...

Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould require the crystal structure of the Ydc2–junction complex. In this study the function of several ... Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs[19], which is reminiscent of the structure of ... 8could play an important functional role, such as the correctpositioning of the SAP motifs in relation to the rest of the protein as well as for the stability of the protein. The truncated Ydc2 mutant...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... the present results indicate that the maximal rate of the mutantEF-Ts in protein synthesis in vivo is  70%ofthatofthewild-type EF-Ts. The studies of the effect of the EF-Ts mutation understarvation ... point of growth inhibition of GRd.tsf(D420¼ 0.47), represented by the D420value of the culture at the interception point between the exponential and the Ôstarvation phaseÕ of growth, is 78% of ... between the levels and the productionpatterns of the (p)ppGpp in the two strains, either beforeor during the full stringent response (Fig. 4B). Growth rates in the presence of kirromycin The effect...

Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot

... binding of the ligand to the other repeats that share a common epi-tope. Other inhibition mechanisms cannot be excluded;among these, there is the possibility that, through the effect of the proximities ... the proximities of the antibody-binding and Fn-binding sites, the interaction of the repeat with Fnmay be sterically hindered in the presence of the anti-body. The results of this study allow ... ﬁbronectin-binding siteto the C-terminal segment of the repeat. The distinct localization of the 15E11 epitope and the ﬁbronectin-binding site suggests that the interferingeffect of the antibody might...

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