Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot
Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot
... Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans Elke Zameitat1, Zoran Gojkovic´2,*, ... DHODH, the yieldsFig. 1. Scheme of dihydroorotate dehydrogenase (DHODH) reactionwith chemical formulae. Electron transfer from dihydroorotate toFMN and further on to quinone. Candida albicans dihydroorotate ... complexes, the assemblyensures the transfer of electrons from different sourcesto oxygen by the respiratory chain complexes and the coupling of proton uptake from the matrix compart-ment [28].The...
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Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt
... PCR’, using the first PCR product astemplate. To make use of the BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product of the nes-ted PCR was replaced with the corresponding ... molecular ratio of 1 : 1000,and further tested for the Met-degrading activity on the basis of the detection of a-keto acid after the addi-tion of 2 mm Met (Fig. 3). Approximately 85% of EhMGL2 activity ... degradation of the 35 kDa band excised from the SDS ⁄ PAGE gel, andpostulated that the truncation was caused by a fortu-itous initiation of translation at Met45 due to the simi-larity of the nucleotide...
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Báo cáo khoa học: Conformational stability of 17b-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus pptx
... 8.5, we studied thermalstability of 17b-HSDcl from CD and UV meltingcurves, from which the temperature of denaturation(Td) and the van’t Hoff enthalpy of denaturation(DH°vH )of1 7b-HSDcl were ... Inspection of Fig. 2B reveals two transitions, the first in the pH range pH 7–5, and the second in the pH range 5–3. The observed transitions in the aci-dic pH range are likely to be the result of decreasing the ... temperature. The degree of reversibilitydecreased with the temperature to which the sample of 17b-HSDcl was heated (data not shown). The thermodynamic profile of 17b-HSDcl is given inTable 2. In the...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... 4 °C. The supernatant was transferred to an HPLC vial. The products of the reaction were detected at 260 nm using the HPLC system described above for the synthesis of the CoA-esters. The assay ... mUÆmg)1protein). These results show, that the test measuring the hydratase reaction of AUH indeedis useful for the direct analysis of fibroblast culturesderived from patients. The residual activity within the patient ... glutaconyl-5-CoA. The two isomers were separated from eachother. However, upon repeated analysis of the isolatedcompounds, the same two signals appeared. The twoisomers seem to interconvert into each other...
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Tài liệu Báo cáo khoa học: Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways doc
... kunkelii, the genomes of the members of the mollicutes group (total number, 7 out of 10) are devoid of genes for the biosynthesis of IPP andDMAPP. The same is true for the genome of the archaeonN. ... of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalyticcycle. The substrate binding site of the type II enzymeremains veiled. However, a patch of ... 2661FMN, we observed the appearance of the signals of bothmethyl groups and of the methine group of the enzymat-ically formed DMAPP (Fig. 4A). Concomitantly, the signals of IPP were progressively...
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Báo cáo khoa học: Biochemical characterization of USP7 reveals post-translational modification sites and structural requirements for substrate processing and subcellular localization pptx
... products, the cleavage site protected by the presence of ubiquitin was Ser341, located in the ‘fingers’ region of the catalytic core domain involvedin the recognition of the ubiquitin core. These ... bands of similar identity derived from other USP7 variants.These symbols were also used to represent graphically the cleav-age sites on the schematic view of USP7 shown below. Biochemical characterization ... superfamily of thiol- and metallo proteases specialized in the process-ing of ubiquitin and ubiquitin-like proteins. They areresponsible for the disassembly of ubiquitin chains, andfor the cleavage of...
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Báo cáo khoa học: Biochemical characterization of annexin B1 from Cysticercus cellulosae pdf
... the presence of calcium were carried outwith the addition of 5 mm CaCl2in the buffer. For investi-gation of the thermal denaturation of annexin B1, the CDsignal at 222 nm was monitored from ... Whereas binding of the carbohydrate to the site on the concave surface of the protein mostlikely does not interfere with the essential annexin–membrane association, the site on the convex surfacewould ... asdetermined from the supernatant of the EDTA elutionstep, is only 60–70%. We therefore analysed the heparin Sepharose resin pellet in the EDTA elutionstep and found that not all of the protein...
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Báo cáo khoa học: Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes potx
... been explored. To further characterize the enzymatic properties of plant TPP enzymes, wealso purified recombinant OsTPP1 using the samemethods. Then, the kinetic parameters of these recom-binant ... homogeneity. The size of the purified recombinant enzyme was estimated tobe 45 kDa on SDS ⁄ PAGE, in accordance with the sizededuced from the nucleotide sequence (42.6 kDa)(Fig. 4A). TPP activity was then ... 13.4 s)1,respectively. Therefore, the kcat⁄ Kmvalues of OsTPP1and OsTPP2 were approximately the same. TheseFig. 3. Complementation of the heat-sensitive phenotype of aSa. cerevisiae tps2...
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Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx
... properties of SPC4. The absorption spectrum of purified SPC4 was recorded in 50 mMsodium acetate pH 5. The inset shows the spectrum of the extract-ed heme. (B) MALDI-TOF-MS analysis of SPC4 heme. Characterization ... willobviously affect the shape of the active site, and thuseither increase or decrease the enzyme’s affinity forsubstrate molecules [1]. This hypothesis is further sup-ported by the fact that different ... spectrophotometer at 25 °C. The hemecofactor of the enzyme was extracted with butanone adjus-ted to pH 2.0 with acetic acid. The UV-spectrum of the heme was recorded in the extraction solution....
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Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx
... Many of the tiss ues included in this a nalysis alsoshowed a prevalence of one isoform over the other. Forinstance in the spleen, the short isoform is the predominantAK species , whereas in the ... than the cerebellum and various components of the forebrain. Furthermore, one or the o ther AK isoformpredominates in most tissues, including the brain, where the short isoform is prevalent. The ... and kidney, the longisoform is more abundant. Most brain regions, with the exception of the cerebellum, express detectable levels of only the short isoform. In t he cerebellum, both isoforms arepresent...
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