Báo cáo khoa học: Stimulation of ﬁbroblast proliferation by neokyotorphin requires Ca2+ inﬂux and activation of PKA, CaMK II and MAPK/ERK pdf

... proliferation by neokyotorphin requires Ca2+ inﬂux and activation of PKA, CaMK II and MAPK/ERK Olga V. Sazonova, Elena Yu. Blishchenko, Anna G. Tolmazova, Dmitry P. Khachin,Konstantin V. Leontiev, Andrey ... activation should induce activation of all protein kinases shownto contribute to the neokyotorphin effect. Both PKA and CaMK II are known to be activated by Ca2+ inﬂux, in the case of CaMK II activation ... L929 cells, are PKA, CaMK II and MAPK.DiscussionThe set of mediators involved in the neokyotorphin proliferative effect (Ca2+L-type channels, intracellularCa2+, PKA, CaMK II and MAPK ⁄ Erk)...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13 docx

... no signiﬁcant binding of actin wasobserved in any of the EREs, irrespective of theabsence and presence of E2. Binding of ERa and ERbwas increased in both ERE1 and ERE2 of theHOXC13 promoter ... II (RNAPII) and H3K4-trimethylation level in ERE1 and ERE2. Our resultsdemonstrated that in both ERE1 and ERE2, the levels of RNAPII and H3K4-trimethylation were increased inthe presence of ... speciﬁcfor ERE1, ERE2, ERE3 and ERE4 of theHOXC13 promoter. (B) Dynamics of recruit-ment of ERa,ERb and MLLs (MLL1–MLL4),H3K4-trimethyl and RNAPII into ERE1 and ERE2 of the HOXC13 promoter under...

Báo cáo khoa học: ISC1-encoded inositol phosphosphingolipid phospholipase C is involved in Na+/Li+ halotolerance of Saccharomyces cerevisiae pptx

... beinvolved in stimulation of ENA1 expression and, conse-quently, in mediating Na+ and Li+tolerance in yeast.Deletion of ISC1 distinctly decreased cellular Na+ and Li+tolerance as growth of the ... extrusion of these cations by induction and activation of the speciﬁc, ATP-driven ion pump Ena1pis initiated [21,23–26]. Both pathways of yeast osmoadap-tation have been intensively studied and many ... thedevelopment of yeast halotolerance against Na+ and Li+ions by means of HOG-independent induction of ENA1expression.EXPERIMENTAL PROCEDURESYeast strains, plasmids, chemicals and mediaThe...

Tài liệu Báo cáo khoa học: Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails ppt

... PAP I. To better understandthe mechanism of activation, we have investigatedwhether stimulation of PAP I activity is correlatedwith the afﬁnity of Hfq for the 3¢ end of RNA. Weused an RNA ... pooled and dialyzedFig. 6. Hfq inhibits the addition of A residues by PNPase. Elonga-tion of 5¢ labeled polyadenylated 3¢rpsO mRNA by PNPase was per-formed in the absence and in the presence of ... the 3¢ end of an RNA molecule and its ability tostimulate RNA elongation catalyzed by poly(A)polymerase I. In contrast,formation of the Hfq–RNA complex inhibits elongation of the RNA by polynucleotide...

Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc

... activation of NF-jB. Attenuation of LXF-289 cell proliferation by ATP is mediated by the activation of the MEK ⁄ ERK1 ⁄ 2, PI3K and p38 MAPK pathways. Activation and translocation of the transcription ... II (CaMKII),which is also known to regulate cell cycle progression and proliferation. Inhibition of CaMKII activity by KN-62 (25 lm) completely reduced the inhibition by ATP.The inhibition of ... of ATP and ADP in theinhibition of LXF-289 cell proliferation. Cell cycle analysis revealed that inhibition of prolif-eration of LXF-289 cells by ATP and ADP was medi-ated by retardation of...

Báo cáo khoa học: Stimulation of p-nitrophenylphosphatase activity of Na+ ⁄ K+-ATPase by NaCl with oligomycin or ATP docx

... respectively. Plots and bars represent the means ± SD fromthree determinations.Fig. 11. Proposed models. (1) Activation of pNPPase by K+ and pNPP. (2) Activation of pNPPase by Na+,K+, pNPP and oligomycin.(3) ... simultaneous addition of NaCl and ATP induced convex-shaped activation curves of pNPPase. The combination of 10 mm NaCl and 0.1 mm ATP maximally activated pNPPase.In the presence of ATP and KCl, increasing ... activity and Rb+binding (Fig. 4A,B). Inthe presence of oligomycin, the binding curve of Rb+ and the activation curve of pNPPase showed a convexshape. The peak of both Rb+binding and pNPPaseactivity...

Báo cáo khoa học: Heme oxygenase-1 ⁄p21WAF1 mediates peroxisome proliferator-activated receptor-c signaling inhibition of proliferation of rat pulmonary artery smooth muscle cells pot

... PASMC proliferation. Proliferation of PASMCs is a hallmark of pathogenesis of pulmonary hypertension [1,4]. How-ever, the mechanisms responsible for inhibition of PASMC proliferation by activation ... expression of HO-1 and p21WAF1. We also explored whether theseresponses modulate cell proliferation induced by 5-HT.ResultsEffect of PPARc agonist on HO-1 expression Activation of PPARc by rosiglitazone ... expression of HO-1 (P < 0.01), sug-gesting that activation of PPARc speciﬁcally and effec-tively mediates HO-1 induction in PASMCs.Role of HO-1 in PPARc agonist suppression of proliferation of...

Báo cáo khoa học: Dehydroepiandrosterone inhibits the proliferation and induces the death of HPV-positive and HPV-negative cervical cancer cells through an androgen- and estrogen-receptor independent mechanism pptx

... Montan˜oLF&Lo´pez-Marure R(2005) Dehydroepiandrosterone inhibits the prolifera-tion of HUVEC by enhancing the expression of p53 and p21, restricting the phosphorylation of RB, and isandrogen- and estrogen-receptor ... estrogen and androgen,because use of antagonists to androgen and estrogenreceptors (ﬂutamide and ICI 182,780, respectively), and letrozol, an inhibitor of the aromatase responsiblefor converting androgen ... antiproliferative effect was not abrogated by inhibitors of androgen and estrogen receptors or by an inhibitor of theconversion of testosterone to estradiol, and this effect was associated withan...

Báo cáo khóa học: Stimulation-dependent recruitment of cytosolic phospholipase A2-a to EA.hy.926 endothelial cell membranes leads to calcium-independent association pdf

... whichsuggested that stimulation periods of less than 2 min causedonly partial activation and reversible relocation of cPLA2-aFig. 5. Effects of EGTA and BAPTA-AM on the relocation of cPLA2-a. ... of CaCl2 and EGTA to Buffer B, as determined by theMETLIGprogram. Equivalent amounts of the fractionswere analysed by SDS/PAGE and Western blotting.Temperature-induced phase separation of ... mMEGTAbuffer, homogenized and subfractionated into cytosolic and membrane fractions and cPLA2-a was detected by immunoblotting. (C) QuantificationoftheamountofcPLA2-a present in each of the indicated...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... characterized by a conserved DFD amino acidmotif in subdomain VII of the catalytic domain and by the presence of an amino acid insertion of variablesize between subdomains VII and VIII [8,9]. Such ... and GST-lofMPK6. However, when lofMPK3 and lofMPK6proteins were tested for phosphorylation with OXI1,no phosphorylation of lofMPK3 or lofMPK6 wasobserved (Fig. 5A). On the other hand, when ... necessary for activation of theOXI1 kinase activity.MPK3 and MPK6 phosphorylate OXI1 and PTI1-4in vitroBecause OXI1 has been shown to play a role in the activation of MPK3 and MPK6 in response...

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