... (Invitrogen, Carlsbad, CA, USA) by PCR using sense primer 5¢-ATCAAAACAACCAAAATGGCCAAGTTGACCAGTGC-3¢ and antisense primer5¢-GAATGCGGCCGCTCAGTCCTGCTC CTCGGCCAC-3 ¢,which introduced a NotI restriction ... BamHI5.4kb as the template with sense primer 5¢-GAATGCGGCCGCGAATGTGTGCAAATTGAAGAAC-3¢ and antisense primer5¢-TTCGAGCTCCGGGGAAACGGTGCCAACTT-3¢,whichintroduced a NotI site (underlined) and a ... plasmid (a gift from Dr K. Apt, Martek Biosciences, Columbia,MD, USA) with sense primer SOE-3 (5¢-AAAAATCAACGCTGAACAATGGTGAGCAAAGGGCGAG-3¢) andantisense primer SOE-4 (5¢-GAATGCGGCCGCTTACTTGTAACAGCTCGTCCATG-3¢),...